Abstract
A range of environmental conditions can lead to oxidative stress; thus, a prompt and effective response to oxidative stress is crucial for the survival of plants. Microarray and northern-blot analyses were performed toward the identification of the factors and signaling pathways that enable plants to limit oxidative damage caused by exposure to high light (HL). Arabidopsis plants grown under moderate light (100 micromol m(-2) s(-1)) were exposed to HL (1,000 micromol m(-2) s(-1)) for 1 h. The microarray analyses revealed that exposure of Arabidopsis to HL caused an increase in known antioxidant genes, as well as several unknown genes. Some of these unknown genes had homologies to possible regulatory genes and metabolic enzymes. Furthermore, it was found that a range of chaperones were up-regulated in the HL treatment and that this induction was specifically due to the HL stress. The temporal expression under HL and different oxidative stress conditions of a subset of HL-responsive genes was confirmed via northern-blot analysis. Results from the arrays were also compared with publicly available microarray data sets from a range of different stress conditions at the Arabidopsis Functional Genomics Consortium. This cross comparison enabled the identification of genes that may be induced by changes in redox poise. Finally, to determine if the genes that were differentially expressed by HL stress were under similar transcriptional control, we analyzed the promoter sequences for the presence of common motifs.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
High light exposure increased expression of known antioxidant genes in Arabidopsis relative to moderate light.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- exposed to HL (1,000 micromol m(-2) s(-1)) for 1 he1
- Outcome
- known antioxidant genese0
- Direction
- increasee0
- Comparator
- moderate light (100 micromol m(-2) s(-1))e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exposure of Arabidopsis to HL caused an increase in known antioxidant genes”
e1“Arabidopsis plants grown under moderate light (100 micromol m(-2) s(-1)) were exposed to HL (1,000 micromol m(-2) s(-1)) for 1 h”
Claim · model-written summary
Chaperones were up-regulated under the HL treatment, and this induction was specifically due to HL stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- HL treatmente2
- Outcome
- chaperonese2
- Direction
- up-regulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Response to High Light in Arabidopsis”
e2“a range of chaperones were up-regulated in the HL treatment and that this induction was specifically due to the HL stress”
Claim · model-written summary
Some unknown HL-responsive genes had homologies to possible regulatory genes and metabolic enzymes.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- unknown genese3
- Outcome
- homologies to possible regulatory genes and metabolic enzymese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Response to High Light in Arabidopsis”
e3“Some of these unknown genes had homologies to possible regulatory genes and metabolic enzymes”
Claim · model-written summary
Temporal expression of a subset of HL-responsive genes under HL and different oxidative stress conditions was confirmed by northern blot.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HL-responsive genese4
- Outcome
- temporal expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Response to High Light in Arabidopsis”
e4“The temporal expression under HL and different oxidative stress conditions of a subset of HL-responsive genes was confirmed via northern-blot analysis”
Claim · model-written summary
Cross comparison with public microarray data identified genes that may be induced by changes in redox poise.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- genes that may be induced by changes in redox poisee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Response to High Light in Arabidopsis”
e5“This cross comparison enabled the identification of genes that may be induced by changes in redox poise”
The same questions in other papers
light · molecular: expression · ArabidopsisPositive and negative results
54 papers in the same direction as this paper · 21 in the opposite direction · 4 reporting no difference from an alternative
- negative
- negative
- positive
76 papers more in this group
See all 80 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.