Abstract
The regeneration potential of shoot apical meristems of soybean, cowpea, peanut, chickpea, and bean was studied on agar-solidified MS nutrient medium supplemented with various concentrations of benzyladenine (BA) and napthaleneacetic acid (NAA) alone or in combination. Soybean plantlets could be regenerated only when 0.05–0.1 μM BA was applied in conjunction with 1 μM NAA. Cowpea meristems did not require exogenously supplied hormones for maximum (100%) plant regeneration to occur. Extremely low levels of BA (0.1–0.005 μM) in association with low levels of NAA (0.05 μM) also induced plant regeneration at very high frequency. Similarly, bean meristems differentiated into plantlets on hormone-free medium or on medium containing only the auxin, NAA. Multiple bud regeneration (15–30 buds per meristem) was induced from bean meristems at high cytokinin levels (10 μM BA). Elongated bean shoots differentiated roots on half-strength MS medium containing 1 μM indoleacetic acid (IAA). Although most combinations of BA or BA and NAA induced shoot regeneration from peanut and chickpea meristems, whole plant regeneration occurred more frequently (75%) from the former only when 0.1 μM BA was applied in combination with 10 μM NAA. Multiple axillary branching occurred from the main shoots regenerated from chickpea meristems; however, rooting occurred only when these shoots were recultured on medium containing 1 μM indolebutyric acid (IBA). Plantlets regenerated from the meristems of all these grain legumes were successfully transferred to pots and grown to maturity.
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Claims 12
Claim · model-written summary
Soybean plantlets regenerated only with BA plus NAA at the stated doses.
- Taxon
- Soybean
Glycine maxe0 - Treatment
- 0.05–0.1 μM BA was applied in conjunction with 1 μM NAAe0
- Outcome
- plantlets could be regeneratede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean plantlets could be regenerated only when 0.05–0.1 μM BA was applied in conjunction with 1 μM NAA.”
Claim · model-written summary
Cowpea meristems achieved maximum 100% regeneration without added hormones.
- Taxon
- Cowpea
Vigna unguiculatae0 - Treatment
- did not require exogenously supplied hormonese0
- Outcome
- plant regeneratione0
- Direction
- maximume0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cowpea meristems did not require exogenously supplied hormones for maximum (100%) plant regeneration to occur.”
Claim · model-written summary
Low BA with low NAA induced very frequent cowpea regeneration.
- Taxon
- cowpeaVigna unguiculatat0
- Treatment
- BA (0.1–0.005 μM) in association with low levels of NAA (0.05 μM)e0
- Outcome
- plant regeneratione0
- Direction
- very high frequencye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Extremely low levels of BA (0.1–0.005 μM) in association with low levels of NAA (0.05 μM) also induced plant regeneration at very high frequency.”
t0“cowpea”
Claim · model-written summary
Bean meristems formed plantlets on hormone-free medium or medium containing NAA alone.
- Taxon
- beane0
- Treatment
- hormone-free medium or on medium containing only the auxin, NAAe0
- Outcome
- differentiated into plantletse0
Not reportedDirection
Verbatim
e0“bean meristems differentiated into plantlets on hormone-free medium or on medium containing only the auxin, NAA.”
Claim · model-written summary
High BA induced 15–30 buds per bean meristem.
Not reportedComparatorDirection
Verbatim
e0“Multiple bud regeneration (15–30 buds per meristem) was induced from bean meristems at high cytokinin levels (10 μM BA).”
Claim · model-written summary
Bean shoots formed roots on half-strength MS medium with IAA.
- Taxon
- beane0
- Treatment
- half-strength MS medium containing 1 μM indoleacetic acid (IAA)e0
- Outcome
- differentiated rootse0
Not reportedDirection
Verbatim
e0“Elongated bean shoots differentiated roots on half-strength MS medium containing 1 μM indoleacetic acid (IAA).”
Claim · model-written summary
Most BA or BA plus NAA combinations induced peanut shoot regeneration.
- Taxon
- peanut
Arachis hypogaeae0 - Treatment
- BA or BA and NAAe0
- Outcome
- shoot regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most combinations of BA or BA and NAA induced shoot regeneration from peanut and chickpea meristems”
Claim · model-written summary
Most BA or BA plus NAA combinations induced chickpea shoot regeneration.
- Taxon
- chickpea
Cicer arietinume0 - Treatment
- BA or BA and NAAe0
- Outcome
- shoot regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most combinations of BA or BA and NAA induced shoot regeneration from peanut and chickpea meristems”
Claim · model-written summary
Peanut whole plant regeneration occurred more often, at 75%, under 0.1 μM BA plus 10 μM NAA.
- Taxon
- peanutArachis hypogaeat0
- Treatment
- 0.1 μM BA was applied in combination with 10 μM NAAe0
- Outcome
- whole plant regeneratione0
- Direction
- more frequentlye0
- Value
- 75%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whole plant regeneration occurred more frequently (75%) from the former only when 0.1 μM BA was applied in combination with 10 μM NAA.”
t0“peanut”
Claim · model-written summary
Chickpea meristem-derived main shoots produced multiple axillary branches.
- Taxon
- chickpeaCicer arietinume0
- Outcome
- Multiple axillary branchinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Multiple axillary branching occurred from the main shoots regenerated from chickpea meristems”
Claim · model-written summary
Chickpea shoots rooted only after reculture with IBA.
- Taxon
- chickpeaCicer arietinumt0
- Treatment
- recultured on medium containing 1 μM indolebutyric acid (IBA)e0
- Outcome
- rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting occurred only when these shoots were recultured on medium containing 1 μM indolebutyric acid (IBA).”
t0“chickpea”
Claim · model-written summary
Regenerated plantlets from all five legumes survived transfer to pots and grew to maturity.
- Taxon
- soybean, cowpea, peanut, chickpea, and beant0
- Outcome
- successfully transferred to pots and grown to maturitye0
Not reportedDirection
Verbatim
e0“Plantlets regenerated from the meristems of all these grain legumes were successfully transferred to pots and grown to maturity.”
t0“soybean, cowpea, peanut, chickpea, and bean”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.