Abstract
Cowpea is an important grain legume crop. The study reports an efficient in vitro multiplication and shoots regeneration protocol from preconditioned embryonic axes of the Turkish cowpea cultivar Akkiz. The embryonic axes were preconditioned with 10 mg/l BA on agar solidified MS medium for 5 days. Thereafter they were cultured on MS medium containing 0.25, 0.50, 0.75 and 1.00 mg/l BA with or without 0.10 mg/l NAA. Mean frequency (%) of shoot regeneration, number of shoots per explant and shoot length decreased with each increase in BA concentration used singly. However, a positive increase was recorded in all parameters in the presence of 0.10 mg/l NAA in the regeneration medium. A maximum mean number of 10.33 shoots per explant was recorded on an MS medium containing 1.00 mg/l BA -0.1 mg/l NAA. Regenerated shoots were rooted on an MS medium containing 0.50 mg/l IBA. Rooted plants were acclimatized at room temperature in soil mix contained in pots where they were subjected to an intermittent mistwater spray for 24 h that maintained 90% relative humidity during the first few days which was gradually reduced to 40% for 10 days. All plants flowered and set seeds in a greenhouse after 3 months.
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Claims 5
Claim · model-written summary
Increasing BA alone decreased shoot regeneration frequency, shoot number and shoot length.
- Taxon
- cowpea
Vigna unguiculatat0 - Material
- Akkizt0
- Treatment
- BA concentration used singlye0
- Outcome
- frequency (%) of shoot regeneration, number of shoots per explant and shoot lengthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mean frequency (%) of shoot regeneration, number of shoots per explant and shoot length decreased with each increase in BA concentration used singly.”
t0“cowpea (Vigna unguiculata L.) cultivar Akkiz”
Claim · model-written summary
NAA at 0.10 mg/l improved all regeneration parameters.
- Taxon
- cowpeaVigna unguiculatat0
- Material
- Akkizt0
- Treatment
- 0.10 mg/l NAAe0
- Outcome
- all parameterse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a positive increase was recorded in all parameters in the presence of 0.10 mg/l NAA in the regeneration medium.”
t0“cowpea (Vigna unguiculata L.) cultivar Akkiz”
Claim · model-written summary
Medium with 1.00 mg/l BA and 0.1 mg/l NAA produced the maximum 10.33 shoots per explant.
- Taxon
- cowpeaVigna unguiculatat0
- Material
- Akkizt0
- Treatment
- MS medium containing 1.00 mg/l BA -0.1 mg/l NAAe0
- Outcome
- mean number of 10.33 shoots per explante0
- Direction
- maximume0
- Value
- 10.33 shoots per explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A maximum mean number of 10.33 shoots per explant was recorded on an MS medium containing 1.00 mg/l BA -0.1 mg/l NAA.”
t0“cowpea (Vigna unguiculata L.) cultivar Akkiz”
Claim · model-written summary
Regenerated shoots rooted on medium containing 0.50 mg/l IBA.
- Taxon
- cowpeaVigna unguiculatat0
- Material
- Akkizt0
- Treatment
- MS medium containing 0.50 mg/l IBAe0
- Outcome
- Regenerated shoots were rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated shoots were rooted on an MS medium containing 0.50 mg/l IBA.”
t0“cowpea (Vigna unguiculata L.) cultivar Akkiz”
Claim · model-written summary
All regenerated plants flowered and set seeds in a greenhouse after three months.
- Taxon
- cowpeaVigna unguiculatat0
- Material
- Akkizt0
- Outcome
- flowered and set seedse0
- Qualifier
- after 3 monthse0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All plants flowered and set seeds in a greenhouse after 3 months.”
t0“cowpea (Vigna unguiculata L.) cultivar Akkiz”
The same questions in other papers
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