Paper · 2012 · Pharmaceutical biotechnology

High Frequency Induction of Hairy Roots and Effect of NAA and 2,4-D on Peanut Hairy Roots Growth and Resveratrol Content

Yu Min

5
claims
11
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

To obtain induction conditions about a high induction rate of peanut hairy root,and to explore the influence of biomass and resveratrol content of peanut hairy root with different concentrations of naphthalene acetic acid(NAA) and 2,4-phenoxyacetic acid(2,4-D),the effects of induced rate of peanut hairy roots with different strains of Agrobacterium rhizogenes and different acetosyringone concentrations were studied and Ms solid medium was used as control,then different concentrations of NAA and 2,4-D were added to cultivate peanut hairy roots,and to explore the influence of hairy root biomass and resveratrol content.The results showed that the induction rate of peanut hairy root reached 92.3%.With increasing NAA concentration,no significant difference was observed in the biomass accumulation between the control and the group treated with various concentrations of NAA.With NAA concentration more and more higher,compared with control,resveratrol content was significantly increased,showing a significant difference,but the resveratrol content had no significant difference in the different concentrations of NAA.Adding 2,4-D,the biomass is significantly lower,the resveratrol content had no difference compared with NAA group.The leaves of peanut were infected by Agrobacterium rhizogenes R1601(A600=0.8),and cultivated the infected leaves on MS solid medium that contained 50 μmol/L acetosyringone,the hairy roots of peanut can be efficiently induced and NAA can serve as an external hormones to increase the resveratrol content in hairy roots of peanut.

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Claims 5

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Peanut hairy root induction rate reached 92.3%.

Outcome
induction rate of peanut hairy roote1
Value
92.3%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e1

“the induction rate of peanut hairy root reached 92.3%”

✓ located verbatim in the source · abstract, characters 648–701 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

NAA at the tested concentrations did not change peanut hairy root biomass accumulation compared with control.

Treatment
NAAe2
Outcome
biomass accumulatione2
Direction
no significant differencee2
Comparator
controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e7

“the influence of biomass and resveratrol content of peanut hairy root”

✓ located verbatim in the source · abstract, characters 95–164 · show in text ↑
e2

“no significant difference was observed in the biomass accumulation between the control and the group treated with various concentrations of NAA”

✓ located verbatim in the source · abstract, characters 736–879 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Increasing NAA concentration significantly increased resveratrol content in peanut hairy roots compared with control.

Organ / stage
hairy rootse4
Treatment
NAAe5
Outcome
resveratrol contente3
Direction
significantly increasede3
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e5

“NAA concentration more and more higher”

✓ located verbatim in the source · abstract, characters 885–923 · show in text ↑
e3

“compared with control, resveratrol content was significantly increased”

✓ located after whitespace/quote normalisation · abstract, characters 924–993 · show in text ↑
e4

“increase the resveratrol content in hairy roots of peanut”

✓ located verbatim in the source · abstract, characters 1509–1566 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Adding 2,4-D significantly lowered peanut hairy root biomass.

Treatment
2,4-De5
Outcome
biomasse5
Direction
significantly lowere5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e7

“the influence of biomass and resveratrol content of peanut hairy root”

✓ located verbatim in the source · abstract, characters 95–164 · show in text ↑
e5

“Adding 2,4-D, the biomass is significantly lower”

✓ located after whitespace/quote normalisation · abstract, characters 1124–1171 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Adding 2,4-D did not change resveratrol content compared with the NAA group.

Treatment
2,4-De5
Outcome
resveratrol contente6
Direction
no differencee6
Comparator
NAA groupe6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e7

“the influence of biomass and resveratrol content of peanut hairy root”

✓ located verbatim in the source · abstract, characters 95–164 · show in text ↑
e5

“Adding 2,4-D, the biomass is significantly lower”

✓ located after whitespace/quote normalisation · abstract, characters 1124–1171 · show in text ↑
e6

“the resveratrol content had no difference compared with NAA group”

✓ located verbatim in the source · abstract, characters 1172–1237 · show in text ↑

1-naphthaleneacetic acid · growth · Arachis hypogaea (peanut)Results differ

22 papers: 20 positive, 2 negative or no effect

1 paper in the same direction as this paper · 20 in the opposite direction

  1. positive
  2. positive
  3. positive

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2,4-D · molecular: metabolites · Arachis hypogaea (peanut)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 reporting no difference from an alternative

  1. positive
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2,4-D · growth · Arachis hypogaea (peanut)Positive and negative results

16 papers: 12 positive, 4 negative or no effect

3 papers in the same direction as this paper · 12 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

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1-naphthaleneacetic acid · molecular: metabolites · Arachis hypogaea (peanut)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.