Abstract
Vigna unguiculata is an important food legume crop in the semiarid tropics. It suffers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. Shoot regeneration from unsliced and longitudinally sliced cotyledon node explants obtained from 3-day-old to 5-day-old seedlings grown in vitro was achieved on Murashige and Skoog (MS) medium containing 1.11, 2.22, 3.33, and 4.44 \muM benzylaminopurine (BA) supplemented with 1.0 mg/L polyvinylpyrrolidone and 500 mg/L bacteriostatic Augmentin. Callus induction was recorded in all culture media on both explants. The shoot regeneration frequency (%) of longitudinally sliced cotyledon node explants was 2-fold to 3-fold higher than that of unsliced cotyledon node explants. A maximum number of 9.92 shoots per longitudinally sliced cotyledon node explant was recorded on MS medium containing 3.33 \muM BA. Each increase in the BA concentration of the culture medium proportionally decreased the mean shoot length on both explants. Maximum mean shoot lengths of 2.80 cm on unsliced cotyledon nodes and 3.04 cm on longitudinally sliced cotyledon node explant were recorded on MS medium containing 1.11 \muM BA. Regenerated shoots were rooted on MS rooting medium containing 2.45 \muM indole-3-butyric acid. In vitro regenerated plants were acclimatised at room temperature in growth rooms, where they produced viable seeds.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
In Vigna unguiculata, the shoot regeneration frequency of longitudinally sliced cotyledon node explants was 2- to 3-fold higher than that of unsliced explants.
- Taxon
- Vigna unguiculata
Vigna unguiculatat0 - Treatment
- longitudinally sliced cotyledon node explantse0
- Outcome
- shoot regeneration frequencye0
- Direction
- highere0
- Value
- 2-fold to 3-folde0
- Comparator
- unsliced cotyledon node explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“The shoot regeneration frequency (%) of longitudinally sliced cotyledon node explants was 2-fold to 3-fold higher than that of unsliced cotyledon node explants”
Claim · model-written summary
A maximum of 9.92 shoots per longitudinally sliced cotyledon node explant was recorded on MS medium with 3.33 \muM BA.
- Taxon
- Vigna unguiculataVigna unguiculatat0
- Treatment
- MS medium containing 3.33 \muM BAe0
- Outcome
- shoots per longitudinally sliced cotyledon node explante0
- Direction
- maximume0
- Value
- 9.92e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“A maximum number of 9.92 shoots per longitudinally sliced cotyledon node explant was recorded on MS medium containing 3.33 \muM BA”
Claim · model-written summary
Each increase in the BA concentration of the culture medium proportionally decreased mean shoot length on both explant types.
- Taxon
- Vigna unguiculataVigna unguiculatat0
- Treatment
- increase in the BA concentratione0
- Outcome
- mean shoot lengthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“Each increase in the BA concentration of the culture medium proportionally decreased the mean shoot length on both explants”
Claim · model-written summary
Maximum mean shoot length on unsliced cotyledon nodes was 2.80 cm on MS medium with 1.11 \muM BA.
- Taxon
- Vigna unguiculataVigna unguiculatat0
- Organ / stage
- unsliced cotyledon nodese0
- Treatment
- MS medium containing 1.11 \muM BAe0
- Outcome
- Maximum mean shoot lengthse0
- Direction
- Maximume0
- Value
- 2.80 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“Maximum mean shoot lengths of 2.80 cm on unsliced cotyledon nodes and 3.04 cm on longitudinally sliced cotyledon node explant were recorded on MS medium containing 1.11 \muM BA”
Claim · model-written summary
Maximum mean shoot length on longitudinally sliced cotyledon node explants was 3.04 cm on MS medium with 1.11 \muM BA.
- Taxon
- Vigna unguiculataVigna unguiculatat0
- Organ / stage
- longitudinally sliced cotyledon node explante0
- Treatment
- MS medium containing 1.11 \muM BAe0
- Outcome
- Maximum mean shoot lengthse0
- Direction
- Maximume0
- Value
- 3.04 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“Maximum mean shoot lengths of 2.80 cm on unsliced cotyledon nodes and 3.04 cm on longitudinally sliced cotyledon node explant were recorded on MS medium containing 1.11 \muM BA”
Claim · model-written summary
Regenerated Vigna unguiculata shoots were rooted on MS rooting medium containing 2.45 \muM indole-3-butyric acid.
- Taxon
- Vigna unguiculataVigna unguiculatat0
- Treatment
- MS rooting medium containing 2.45 \muM indole-3-butyric acide0
- Outcome
- Regenerated shoots were rootede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“Regenerated shoots were rooted on MS rooting medium containing 2.45 \muM indole-3-butyric acid”
Claim · model-written summary
In vitro regenerated plants were acclimatised in growth rooms, where they produced viable seeds.
- Taxon
- Vigna unguiculataVigna unguiculatat0
- Outcome
- produced viable seedse0
- Setting
- growth roomse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Vigna unguiculata”
e0“In vitro regenerated plants were acclimatised at room temperature in growth rooms, where they produced viable seeds”
The same questions in other papers
N-benzyladenine · growth · Vigna unguiculata (cowpea)Positive and negative results
1 paper in the same direction as this paper · 11 in the opposite direction
- negative
- positive
- positive
9 papers more in this group
See all 13 papers →in vitro · growth · Vigna unguiculata (cowpea)Positive and negative results
11 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- negative
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.