Abstract
The growth rate of cultured free-cells and their contents of chlorophyll, Mg, K and N reached peaks on media containing ΣMn+ = 60 and 80 meq per liter, or in which πsalts=2.7 and 3.6 atm. These concentration levels in the media were considerably higher than those (πSalts=1.5–2.0 atm) which produced good growth of intact plants. This discrepancy in media concentrations may be due to differences in the culture conditions and in the morphology of the materials. Very low Ca2+/(ΣMn+−Ca2+) equivalent ratios of 0.1 to 0.01 in a medium were sufficient for good growth of free cells, while generally about unity of the ratio is required in a medium for good growth of intact plants. The difference in the medium composition may also be due to morphological differences in the materials. Addition of Cl− to the medium further promoted the growth rate and friability of cell clumps. A low concentration of 0.01 mM BO33− in the medium also promoted the friability of cell clumps.
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Claims 7
Claim · model-written summary
Growth rate of cultured tobacco cells reached a peak on media with ΣMn+ = 60 and 80 meq per liter, or πsalts of 2.7 and 3.6 atm.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- cultured free-cellse0
- Treatment
- media containing ΣMn+ = 60 and 80 meq per litere0
- Outcome
- growth ratee0
- Direction
- reached peakse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e0“The growth rate of cultured free-cells and their contents of chlorophyll, Mg, K and N reached peaks on media containing ΣMn+ = 60 and 80 meq per liter, or in which πsalts=2.7 and 3.6 atm”
Claim · model-written summary
Chlorophyll content of cultured tobacco cells reached a peak on the same media.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultured free-cellse0
- Treatment
- media containing ΣMn+ = 60 and 80 meq per litere0
- Outcome
- contents of chlorophyll, Mge0
- Direction
- reached peakse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e0“The growth rate of cultured free-cells and their contents of chlorophyll, Mg, K and N reached peaks on media containing ΣMn+ = 60 and 80 meq per liter, or in which πsalts=2.7 and 3.6 atm”
Claim · model-written summary
Mg and N contents peaked on media with ΣMn+ of 60 and 80 meq per liter, or πsalts of 2.7 and 3.6 atm.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- cultured free-cellse0
- Treatment
- media containing ΣMn+ = 60 and 80 meq per litere0
- Outcome
- K and Ne0
- Direction
- reached peakse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e0“The growth rate of cultured free-cells and their contents of chlorophyll, Mg, K and N reached peaks on media containing ΣMn+ = 60 and 80 meq per liter, or in which πsalts=2.7 and 3.6 atm”
Claim · model-written summary
Medium concentrations supporting good growth of cultured tobacco cells were considerably higher than those supporting intact plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- πsalts=2.7 and 3.6 atme0
- Outcome
- concentration levelse1
- Direction
- considerably highere1
- Comparator
- those (πSalts=1.5–2.0 atm)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e0“The growth rate of cultured free-cells and their contents of chlorophyll, Mg, K and N reached peaks on media containing ΣMn+ = 60 and 80 meq per liter, or in which πsalts=2.7 and 3.6 atm”
e1“These concentration levels in the media were considerably higher than those (πSalts=1.5–2.0 atm) which produced good growth of intact plants”
Claim · model-written summary
Very low Ca2+ to other cation ratios of 0.1 to 0.01 were sufficient for good growth of cultured tobacco cells, unlike the unity ratio needed by intact plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Ca2+/(ΣMn+−Ca2+) equivalent ratios of 0.1 to 0.01e2
- Outcome
- good growth of free cellse2
- Direction
- sufficiente2
- Comparator
- about unity of the ratioe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e2“Very low Ca2+/(ΣMn+−Ca2+) equivalent ratios of 0.1 to 0.01 in a medium were sufficient for good growth of free cells, while generally about unity of the ratio is required in a medium for good growth of intact plants”
Claim · model-written summary
Adding Cl− to the medium promoted the growth rate and friability of cultured tobacco cell clumps.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Addition of Cl− to the mediume3
- Outcome
- growth rate and friability of cell clumpse3
- Direction
- promotede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e3“Addition of Cl− to the medium further promoted the growth rate and friability of cell clumps”
Claim · model-written summary
A low concentration of 0.01 mM BO33− promoted the friability of cultured tobacco cell clumps.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- A low concentration of 0.01 mM BO33−e4
- Outcome
- friability of cell clumpse4
- Direction
- promotede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“The mineral nutrition of cultured chlorophyllous cells of tobacco”
e4“A low concentration of 0.01 mM BO33− in the medium also promoted the friability of cell clumps”
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31 papers in the same direction as this paper · 8 in the opposite direction
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See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.