Paper · 1988 · Plant and Cell Physiology

Immunochemical Difference of Wound-Induced 1-Aminocyclopropane-1-carboxylate Synthase from the Auxin-Induced Enzyme

Naoki Nakagawa; Nobuyoshi Nakajima; Hidemasa Imaseki

6
claims
7
verbatim quotes
24
citations
1
research areas
—
Not open access

Abstract

Immunochemical cross-reactivity of wound- and auxin-induced 1-aminocyclopropane-1-carboxylate (ACC) synthase was examined with the antibody against wound-induced ACC synthase purified from mesocarp of winter squash (Cucurbita maxima Duch.). The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean. These results indicate that the primary structure of the wound-induced enzyme is different from that of the auxin-induced enzyme in the same species, and implies that there are two different genes for ACC synthase, one for wound induction and the other for auxin induction.

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Claims 6

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Antibody against wound-induced ACC synthase recognized the enzyme from wounded winter squash hypocotyls.

Taxon
winter squashCucurbita maximae1
Treatment
wounded hypocotylse1
Outcome
recognized ACC synthasee1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean”

✓ located verbatim in the source · abstract, characters 241–448 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Antibody against wound-induced ACC synthase recognized the enzyme from wounded tomato pericarp.

Treatment
wounded pericarpe1
Outcome
recognized ACC synthasee1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean”

✓ located verbatim in the source · abstract, characters 241–448 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Antibody against wound-induced ACC synthase did not recognize the enzyme from IAA-treated winter squash hypocotyls.

Taxon
winter squashCucurbita maximae1
Treatment
IAA-treated hypocotylse1
Outcome
recognized ACC synthasee1
Comparator
wounded hypocotylse1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean”

✓ located verbatim in the source · abstract, characters 241–448 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Antibody against wound-induced ACC synthase did not recognize the enzyme from IAA-treated tomato.

Treatment
IAA-treatede1
Outcome
recognized ACC synthasee1
Comparator
wounded pericarpe1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean”

✓ located verbatim in the source · abstract, characters 241–448 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Antibody against wound-induced ACC synthase did not recognize the enzyme from IAA-treated mung bean.

Taxon
mung beanVigna radiatae1
Treatment
IAA-treatede1
Outcome
recognized ACC synthasee1
Comparator
wounded pericarpe1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean”

✓ located verbatim in the source · abstract, characters 241–448 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

The primary structure of the wound-induced ACC synthase differs from that of the auxin-induced enzyme in the same species.

Taxon
winter squashCucurbita maximae1
Gene
wound-induced enzymee2
Outcome
primary structure of the wound-induced enzymee2
Qualifier
different from that of the auxin-induced enzymee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗

Verbatim

e1

“The antibody recognized ACC synthase from wounded hypocotyls of winter squash and from wounded pericarp of tomato fruits, but not the enzyme from IAA-treated hypocotyls of winter squash, tomato and mung bean”

✓ located verbatim in the source · abstract, characters 241–448 · show in text ↑
e2

“These results indicate that the primary structure of the wound-induced enzyme is different from that of the auxin-induced enzyme in the same species”

✓ located verbatim in the source · abstract, characters 450–598 · show in text ↑

indole-3-acetic acid · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 reporting no difference from an alternative

  1. positive
  2. positive
  3. negative
See all 4 papers →

indole-3-acetic acid · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results

9 papers: 7 positive, 2 negative or no effect

1 paper in the same direction as this paper · 7 reporting no difference from an alternative

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.