Abstract
Auxin is known to stimulate greatly both C(2)H(4) production and the conversion of methionine to ethylene in vegetative tissues, while amino-ethoxyvinylglycine (AVG) or Co(2+) ion effectively block these processes. To identify the step in the ethylene biosynthetic pathway at which indoleacetic acid (IAA) and AVG exert their effects, [3-(14)C]methionine was administered to IAA or IAA-plus-AVG-treated mung bean hypocotyls, and the conversion of methionine to S-adenosylmethionine (SAM), 1-amino-cyclopropane-1-carboxylic acid (ACC), and C(2)H(4) was studied. The conversion of methionine to SAM was unaffected by treatment with IAA or IAA plus AVG, but active conversion of methionine to ACC was found only in tissues which were treated with IAA and which were actively producing ethylene. AVG treatment abolished both the conversion of methionine to ACC and ethylene production. These results suggest that in the ethylene biosynthetic pathway (methionine --> SAM --> ACC --> C(2)H(4)) IAA stimulates C(2)H(4) production by inducing the synthesis or activation of ACC synthase, which catalyzes the conversion of SAM to ACC. Indeed, ACC synthase activity was detected only in IAA-treated tissues and its activity was completely inhibited by AVG. This conclusion was supported by the observation that endogenous ACC accumulated after IAA treatment, and that this accumulation was completely eliminated by AVG treatment. The characteristics of Co(2+) inhibition of IAA-dependent and ACC-dependent ethylene production were similar. The data indicate that Co(2+) exerts its effect by inhibiting the conversion of ACC to ethylene. This conclusion was further supported by the observation that when Co(2+) was administered to IAA-treated tissues, endogenous ACC accumulated while ethylene production declined.
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Claims 14
Claim · model-written summary
IAA did not alter methionine conversion to SAM.
- Taxon
- mung bean
.jpg?width=60)
Vigna radiatae1 - Treatment
- IAAe0
- Outcome
- conversion of methionine to SAMe0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The conversion of methionine to SAM was unaffected by treatment with IAA or IAA plus AVG”
e1“mung bean hypocotyls”
Claim · model-written summary
IAA plus AVG did not alter methionine conversion to SAM.
- Taxon
- mung beanVigna radiatae1
- Treatment
- IAA plus AVGe0
- Outcome
- conversion of methionine to SAMe0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The conversion of methionine to SAM was unaffected by treatment with IAA or IAA plus AVG”
e1“mung bean hypocotyls”
Claim · model-written summary
Active methionine to ACC conversion occurred only in IAA-treated ethylene-producing tissues.
- Taxon
- mung beanVigna radiatae1
- Treatment
- IAAe0
- Outcome
- active conversion of methionine to ACCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“active conversion of methionine to ACC was found only in tissues which were treated with IAA and which were actively producing ethylene”
e1“mung bean hypocotyls”
Claim · model-written summary
AVG abolished methionine to ACC conversion.
- Taxon
- mung beanVigna radiatae1
- Treatment
- AVG treatmente0
- Outcome
- conversion of methionine to ACCe0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AVG treatment abolished both the conversion of methionine to ACC and ethylene production”
e1“mung bean hypocotyls”
Claim · model-written summary
AVG abolished ethylene production.
- Taxon
- mung beanVigna radiatae1
- Treatment
- AVG treatmente0
- Outcome
- ethylene productione0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AVG treatment abolished both the conversion of methionine to ACC and ethylene production”
e1“mung bean hypocotyls”
Claim · model-written summary
ACC synthase activity was detected only after IAA treatment.
- Taxon
- mung beanVigna radiatae1
- Gene
- ACC synthasee0
- Outcome
- ACC synthase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC synthase activity was detected only in IAA-treated tissues”
e1“mung bean hypocotyls”
Claim · model-written summary
AVG completely inhibited ACC synthase activity.
- Taxon
- mung beanVigna radiatae2
- Treatment
- AVGe0
- Gene
- ACC synthasee1
- Outcome
- activitye0
- Direction
- completely inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its activity was completely inhibited by AVG”
e1“ACC synthase activity was detected only in IAA-treated tissues”
e2“mung bean hypocotyls”
Claim · model-written summary
Endogenous ACC accumulated after IAA treatment.
- Taxon
- mung beanVigna radiatae1
- Treatment
- IAA treatmente0
- Outcome
- endogenous ACCe0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous ACC accumulated after IAA treatment”
e1“mung bean hypocotyls”
Claim · model-written summary
AVG eliminated the IAA-induced ACC accumulation.
- Taxon
- mung beanVigna radiatae2
- Treatment
- AVG treatmente0
- Outcome
- accumulatione0
- Direction
- completely eliminatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this accumulation was completely eliminated by AVG treatment”
e1“endogenous ACC accumulated after IAA treatment”
e2“mung bean hypocotyls”
Claim · model-written summary
Cobalt inhibition was similar for IAA-dependent and ACC-dependent ethylene production.
- Taxon
- mung beanVigna radiatae1
- Treatment
- Co(2+)e0
- Outcome
- ethylene productione0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The characteristics of Co(2+) inhibition of IAA-dependent and ACC-dependent ethylene production were similar”
e1“mung bean hypocotyls”
Claim · model-written summary
Cobalt caused ACC accumulation in IAA-treated tissues.
- Taxon
- mung beanVigna radiatae1
- Treatment
- Co(2+)e0
- Outcome
- endogenous ACCe0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when Co(2+) was administered to IAA-treated tissues, endogenous ACC accumulated while ethylene production declined”
e1“mung bean hypocotyls”
Claim · model-written summary
Cobalt lowered ethylene production in IAA-treated tissues.
- Taxon
- mung beanVigna radiatae1
- Treatment
- Co(2+)e0
- Outcome
- ethylene productione0
- Direction
- declinede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when Co(2+) was administered to IAA-treated tissues, endogenous ACC accumulated while ethylene production declined”
e1“mung bean hypocotyls”
Claim · model-written summary
IAA was inferred to induce or activate ACC synthase.
- Taxon
- mung beanVigna radiatae1
- Treatment
- IAAe0
- Gene
- ACC synthasee0
- Outcome
- C(2)H(4) productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA stimulates C(2)H(4) production by inducing the synthesis or activation of ACC synthase”
e1“mung bean hypocotyls”
Claim · model-written summary
Cobalt was inferred to inhibit conversion of ACC to ethylene.
- Taxon
- mung beanVigna radiatae1
- Treatment
- Co(2+)e0
- Outcome
- conversion of ACC to ethylenee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co(2+) exerts its effect by inhibiting the conversion of ACC to ethylene”
e1“mung bean hypocotyls”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative
- no effect
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- positive
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See all 9 papers →co2 · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
1 paper more in this group
See all 5 papers →co2 · physiology: hormones · Vigna radiata (mung bean)Results agree
2 papers in the same direction as this paper
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.