Abstract
Mg++-activated (A) and -inhibited (B) ATPases were isolated from dark-grown mung bean hypocotyls and the effect of in vivo and in vitro IAA-treatment on these ATPases was observed. The activity of the A-ATPase, which was localized in the plasma membrane-rich fractions, was enhanced by exogenously added l0−10M IAA (ca. 35%). The stimulatory effect of in vitro IAA-treatment was particularly found in the A ATPase isolated from sections treated with 10−5M IAA for 60 min (ca. 280%). The activity of the B-ATPase, which was localized in the soluble fraction of the cell homogenate, was markedly increased by in vivo treatment with 10−7M IAA for 60 min (ca. 360%). The stimulatory effect of IAA-treatment was observed even if protein synthesis was inhibited by cycloheximide. These results suggest that the enhancement of the activities of the A- and B-ATPases caused by in vivo and in vitro IAA-treatments occur as a result of the activation of already present enzymes, rather than of the synthesis of these enzymes.
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Claims 5
Claim · model-written summary
Exogenous IAA enhanced A-ATPase activity by about 35%.
- Taxon
- mung bean
.jpg?width=60)
Vigna radiatat0 - Organ / stage
- hypocotylst0
- Treatment
- exogenously added l0−10M IAAe0
- Gene
- A-ATPasee0
- Outcome
- activity of the A-ATPasee0
- Direction
- enhancede0
- Value
- ca. 35%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“mung bean hypocotyls”
e0“The activity of the A-ATPase, which was localized in the plasma membrane-rich fractions, was enhanced by exogenously added l0−10M IAA (ca. 35%).”
Claim · model-written summary
In vitro IAA stimulation of A-ATPase was particularly found after section treatment with 10−5M IAA for 60 min, at about 280%.
- Taxon
- mung beanVigna radiatat0
- Treatment
- sections treated with 10−5M IAA for 60 mine0
- Gene
- A ATPasee0
- Outcome
- stimulatory effect of in vitro IAA-treatmente0
- Direction
- stimulatorye0
- Value
- ca. 280%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mung bean hypocotyls”
e0“The stimulatory effect of in vitro IAA-treatment was particularly found in the A ATPase isolated from sections treated with 10−5M IAA for 60 min (ca. 280%).”
Claim · model-written summary
In vivo 10−7M IAA for 60 min markedly increased B-ATPase activity by about 360%.
- Taxon
- mung beanVigna radiatat0
- Treatment
- in vivo treatment with 10−7M IAA for 60 mine0
- Gene
- B-ATPasee0
- Outcome
- activity of the B-ATPasee0
- Direction
- increasede0
- Value
- ca. 360%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mung bean hypocotyls”
e0“The activity of the B-ATPase, which was localized in the soluble fraction of the cell homogenate, was markedly increased by in vivo treatment with 10−7M IAA for 60 min (ca. 360%).”
Claim · model-written summary
IAA stimulation persisted when cycloheximide inhibited protein synthesis.
- Taxon
- mung beanVigna radiatat0
- Treatment
- IAA-treatmente0
- Outcome
- stimulatory effecte0
- Qualifier
- protein synthesis was inhibited by cycloheximidee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mung bean hypocotyls”
e0“The stimulatory effect of IAA-treatment was observed even if protein synthesis was inhibited by cycloheximide.”
Claim · model-written summary
The authors infer that IAA activates existing ATPases rather than inducing enzyme synthesis.
- Taxon
- mung beanVigna radiatat0
- Treatment
- in vivo and in vitro IAA-treatmentse0
- Gene
- A- and B-ATPasese0
- Outcome
- enhancement of the activities of the A- and B-ATPasese0
- Qualifier
- activation of already present enzymes, rather than of the synthesis of these enzymese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“mung bean hypocotyls”
e0“the enhancement of the activities of the A- and B-ATPases caused by in vivo and in vitro IAA-treatments occur as a result of the activation of already present enzymes, rather than of the synthesis of these enzymes.”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Vigna radiata (mung bean)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- no effect
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.