Abstract
High performance liquid chromatography of extracts of tomato (Lycopersicon esculentum Mill.) incubated with a relatively low concentration (4 mum) of [1-(14)C]indole-3-acetic acid (IAA) revealed the presence of two major polar metabolites. Hydrolysis of the two metabolites with 7 n NaOH yielded the same compound, which had a retention time similar to that of ring-expanded oxindole-3-acetic acid (OxIAA) on high performance liquid chromatography. The identity of the indolic moiety of these conjugates as OxIAA was further confirmed by gas chromatography-mass spectrometry. Chromatography of the two OxIAA conjugates on a calibrated Bio-Gel P-2 column indicated that their molecular weights are about 1200 and 1000. Aspartic acid and glutamic acid were the major amino acids detected in acid hydrolysates of the two conjugates. Increasing the concentration of IAA in the incubation medium resulted in an increase in the formation of indole-3-acetylaspartic acid (IAAsp) with a concomitant decrease in the formation of the two OxIAA conjugates. Feeding experiments with labeled IAAsp and OxIAA showed that IAAsp and not OxIAA is the precursor of these conjugates. The data obtained indicate that exogenous IAA is converted in tomato pericarp tissue to high molecular weight conjugates, presumably peptides, of OxIAA via the oxidation of IAAsp. The oxidation of IAAsp seems to be a rate-limiting step in the formation of these conjugates from exogenous IAA.
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Claims 11
Claim · model-written summary
Tomato extracts incubated with labeled IAA contained two major polar metabolites.
- Taxon
- tomato (Lycopersicon esculentum Mill.)
Solanum lycopersicume0 - Treatment
- [1-(14)C]indole-3-acetic acid (IAA)e0
- Outcome
- two major polar metabolitese0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High performance liquid chromatography of extracts of tomato (Lycopersicon esculentum Mill.) incubated with a relatively low concentration (4 mum) of [1-(14)C]indole-3-acetic acid (IAA) revealed the presence of two major polar metabolites.”
Claim · model-written summary
Hydrolysis of both metabolites yielded a compound with retention time similar to OxIAA.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- 7 n NaOHe0
- Outcome
- retention time similar to that of ring-expanded oxindole-3-acetic acid (OxIAA)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrolysis of the two metabolites with 7 n NaOH yielded the same compound, which had a retention time similar to that of ring-expanded oxindole-3-acetic acid (OxIAA) on high performance liquid chromatography.”
t0“Tomato”
Claim · model-written summary
The indolic moiety of the conjugates was identified as OxIAA.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- indolic moiety of these conjugates as OxIAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The identity of the indolic moiety of these conjugates as OxIAA was further confirmed by gas chromatography-mass spectrometry.”
t0“Tomato”
Claim · model-written summary
One OxIAA conjugate had a molecular weight of about 1200.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- molecular weightse0
- Value
- 1200e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromatography of the two OxIAA conjugates on a calibrated Bio-Gel P-2 column indicated that their molecular weights are about 1200 and 1000.”
t0“Tomato”
Claim · model-written summary
One OxIAA conjugate had a molecular weight of about 1000.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- molecular weightse0
- Value
- 1000e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromatography of the two OxIAA conjugates on a calibrated Bio-Gel P-2 column indicated that their molecular weights are about 1200 and 1000.”
t0“Tomato”
Claim · model-written summary
Aspartic and glutamic acids were the major amino acids in conjugate hydrolysates.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- Aspartic acid and glutamic acid were the major amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aspartic acid and glutamic acid were the major amino acids detected in acid hydrolysates of the two conjugates.”
t0“Tomato”
Claim · model-written summary
Higher IAA increased indole-3-acetylaspartic acid (IAAsp)
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Increasing the concentration of IAAe0
- Outcome
- indole-3-acetylaspartic acid (IAAsp)e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the concentration of IAA in the incubation medium resulted in an increase in the formation of indole-3-acetylaspartic acid (IAAsp) with a concomitant decrease in the formation of the two OxIAA conjugates.”
t0“Tomato”
Claim · model-written summary
Higher IAA decreased formation of the two OxIAA conjugates.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Increasing the concentration of IAAe0
- Outcome
- two OxIAA conjugatese0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the concentration of IAA in the incubation medium resulted in an increase in the formation of indole-3-acetylaspartic acid (IAAsp) with a concomitant decrease in the formation of the two OxIAA conjugates.”
t0“Tomato”
Claim · model-written summary
IAAsp, rather than OxIAA, was the precursor of the conjugates.
- Taxon
- TomatoSolanum lycopersicumt0
- Compound
- IAAspe0
- Outcome
- precursor of these conjugatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Feeding experiments with labeled IAAsp and OxIAA showed that IAAsp and not OxIAA is the precursor of these conjugates.”
t0“Tomato”
Claim · model-written summary
Exogenous IAA is converted in tomato pericarp to high molecular weight OxIAA conjugates, presumably peptides, via IAAsp oxidation.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- pericarp tissuee0
- Treatment
- exogenous IAAe0
- Outcome
- high molecular weight conjugatese0
- Qualifier
- via the oxidation of IAAspe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The data obtained indicate that exogenous IAA is converted in tomato pericarp tissue to high molecular weight conjugates, presumably peptides, of OxIAA via the oxidation of IAAsp.”
Claim · model-written summary
IAAsp oxidation appears rate limiting for conjugate formation from exogenous IAA.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- formation of these conjugatese0
- Qualifier
- oxidation of IAAsp seems to be a rate-limiting stepe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oxidation of IAAsp seems to be a rate-limiting step in the formation of these conjugates from exogenous IAA.”
t0“Tomato”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.