Paper · 2007 · Current Science

In vitro clonal propagation of biodiesel plant (Jatropha curcas L.).

Mukul Manjari Datta; Priyanka Mukherjee; Biswajit Ghosh; et al.

8
claims
20
verbatim quotes
93
citations
2
research areas
—
Not open access

Abstract

In the present investigation, in vitro clonal propagation of seven-month-old Jatropha curcas L. was achieved employing nodal explants. Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) basal medium supplemented with 22.2 μM N-benzyladenine (BA) and 55.6 μM adenine sulphate, in which cultures produced 6.2 ± 0.56 shoots per nodal explant with 2.0 ± 0.18 cm average length after 4–6 weeks. The rate of shoot multiplication was significantly enhanced after transfer to MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphate for 4 weeks. Both shoot number (30.8 ± 5.48) and average shoot length (4.8 ± 0.43 cm) were found to increase significantly. About 52% of root induction occurred in MS basal medium supplemented with 1.0 μM IBA in 2–3 weeks. Further elongation of roots with average length of 8.7 ± 1.35 cm was obtained in unsupplemented MS basal medium for 2–3 weeks. The plantlets (12–16week-old) were successfully acclimatized in soil with 87% survival frequency.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Shoot bud proliferation on MS medium with 22.2 μM BA and 55.6 μM adenine sulphate produced 6.2 ± 0.56 shoots per nodal explant.

Taxon
Jatropha curcas L.Jatropha curcast0
Organ / stage
nodal explante1
Treatment
basal medium supplemented with 22.2 μM N-benzyladenine (BA) and 55.6 μM adenine sulphatee0
Outcome
shoots per nodal explante1
Value
6.2 ± 0.56e1
Setting
In vitrot0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e0

“Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) basal medium supplemented with 22.2 μM N-benzyladenine (BA) and 55.6 μM adenine sulphate”

✓ located verbatim in the source · abstract, characters 135–305 · show in text ↑
e1

“in which cultures produced 6.2 ± 0.56 shoots per nodal explant with 2.0 ± 0.18 cm average length after 4–6 weeks”

✓ located verbatim in the source · abstract, characters 307–419 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Shoots from the same medium averaged 2.0 ± 0.18 cm in length after 4–6 weeks.

Taxon
Jatropha curcas L.Jatropha curcast0
Treatment
basal medium supplemented with 22.2 μM N-benzyladenine (BA) and 55.6 μM adenine sulphatee0
Outcome
average lengthe1
Value
2.0 ± 0.18 cme1
Qualifier
after 4–6 weekse1
Setting
In vitrot0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e0

“Axillary shoot bud proliferation was best initiated on Murashige and Skoog’s (MS) basal medium supplemented with 22.2 μM N-benzyladenine (BA) and 55.6 μM adenine sulphate”

✓ located verbatim in the source · abstract, characters 135–305 · show in text ↑
e1

“in which cultures produced 6.2 ± 0.56 shoots per nodal explant with 2.0 ± 0.18 cm average length after 4–6 weeks”

✓ located verbatim in the source · abstract, characters 307–419 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

MS medium with 2.3 μM Kn, 0.5 μM IBA and 27.8 μM adenine sulphate significantly enhanced the rate of shoot multiplication after transfer.

Taxon
Jatropha curcas L.Jatropha curcast0
Treatment
MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphatee2
Outcome
rate of shoot multiplicatione2
Direction
enhancede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e2

“The rate of shoot multiplication was significantly enhanced after transfer to MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphate for 4 weeks”

✓ located verbatim in the source · abstract, characters 421–644 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The shoot number increased significantly, to 30.8 ± 5.48, on the 2.3 μM Kn, 0.5 μM IBA and 27.8 μM adenine sulphate medium.

Taxon
Jatropha curcas L.Jatropha curcast0
Treatment
MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphatee2
Outcome
shoot numbere3
Direction
increasee3
Value
30.8 ± 5.48e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e2

“The rate of shoot multiplication was significantly enhanced after transfer to MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphate for 4 weeks”

✓ located verbatim in the source · abstract, characters 421–644 · show in text ↑
e3

“Both shoot number (30.8 ± 5.48) and average shoot length (4.8 ± 0.43 cm) were found to increase significantly”

✓ located verbatim in the source · abstract, characters 646–755 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The average shoot length increased significantly, to 4.8 ± 0.43 cm, on the 2.3 μM Kn, 0.5 μM IBA and 27.8 μM adenine sulphate medium.

Taxon
Jatropha curcas L.Jatropha curcast0
Treatment
MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphatee2
Outcome
average shoot lengthe3
Direction
increasee3
Value
4.8 ± 0.43 cme3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e2

“The rate of shoot multiplication was significantly enhanced after transfer to MS basal medium supplemented with 2.3 μM 6-furfuryl amino purine (Kn), 0.5 μM indole3-butyric acid (IBA) and 27.8 μM adenine sulphate for 4 weeks”

✓ located verbatim in the source · abstract, characters 421–644 · show in text ↑
e3

“Both shoot number (30.8 ± 5.48) and average shoot length (4.8 ± 0.43 cm) were found to increase significantly”

✓ located verbatim in the source · abstract, characters 646–755 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

About 52% of roots were induced on MS medium with 1.0 μM IBA within 2–3 weeks.

Taxon
Jatropha curcas L.Jatropha curcast0
Treatment
MS basal medium supplemented with 1.0 μM IBAe4
Outcome
root inductione4
Value
52%e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e4

“About 52% of root induction occurred in MS basal medium supplemented with 1.0 μM IBA in 2–3 weeks”

✓ located verbatim in the source · abstract, characters 757–854 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Roots elongated to an average of 8.7 ± 1.35 cm on unsupplemented MS medium within 2–3 weeks.

Taxon
Jatropha curcas L.Jatropha curcast0
Treatment
unsupplemented MS basal mediume5
Outcome
elongation of rootse5
Value
8.7 ± 1.35 cme5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e5

“Further elongation of roots with average length of 8.7 ± 1.35 cm was obtained in unsupplemented MS basal medium for 2–3 weeks”

✓ located verbatim in the source · abstract, characters 856–981 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Plantlets of 12–16 weeks acclimatized in soil with 87% survival.

Taxon
Jatropha curcas L.Jatropha curcast0
Organ / stage
plantlets (12–16week-old)e6
Treatment
acclimatized in soile6
Outcome
survival frequencye6
Value
87%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Jatropha curcas
Jatropha curcas · Taxon · picture from its Wikimedia Commons categoryAdityamadhav83 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“In vitro clonal propagation of biodiesel plant (Jatropha curcas L.)”

✓ located verbatim in the source · title, characters 0–67 · show in text ↑
e6

“The plantlets (12–16week-old) were successfully acclimatized in soil with 87% survival frequency”

✓ located verbatim in the source · abstract, characters 983–1079 · show in text ↑

aminopurine · pest · Jatropha curcasResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

adenine · pest · Jatropha curcasResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

sulfate · pest · Jatropha curcasResults agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

indole-3-butyric acid · pest · Jatropha curcasResults agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

adenine · growth · Jatropha curcasResults agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

sulfate · growth · Jatropha curcasResults agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

indole-3-butyric acid · growth · Jatropha curcasResults agree

24 papers: 24 positive, 0 negative or no effect

23 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

20 papers more in this group

See all 24 papers →

in vitro · growth · Jatropha curcasPositive and negative results

27 papers: 25 positive, 2 negative or no effect

24 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. positive

23 papers more in this group

See all 27 papers →

8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.