Abstract
Jatropha is a drought-tolerant plant producing seed oil for the biodiesel. Limitation to the development of jatropha is unavailability of high-yielding varieties and efficient in vitro regeneration system which is required for micropropagation. In this study, in vitro regeneration system from jatropha juvenile cotyledon was established. Firstly, concentrations of hormones in the MS medium were optimized and it was found that 1.5 mgL-1 benzyl adenine (BA) + 0.05 mgL-1 indole-3-butyric acid (IBA) + 0.5 mgL-1 thidiazuron (TDZ) turned out to be the best for shoot induction (3.82 ± 0.18 shoots/explant). Secondly, shoot induction medium was fortified with different concentrations of glutamine and adenine sulfate. It was found that 25 mgL-1 each of glutamine and adenine sulfate was the most effective, resulting to 9.09 ± 0.37 shoots/explant and 93.0% regeneration frequency. Regenerated shoots were cultured on medium containing 0.5 mgL-1 BA and different concentrations of gibberellic acid (GA3), 0.5 mgL-1 GA3 with 0.5 mgL-1 BA were found to be the best for shoot elongation (2.13 ± 0.18 cm). The highest frequency of root (40%) was observed on the medium with 0.5 mgL-1 IBA. The established procedures will be useful for the mass propagation and genetic transformation of elite jatropha genotypes. Key words: Adenine sulfate (Ads), benzyl adenine (BA), indole-3-butyric acid (IBA), in vitro regeneration, glutamine (Gln).
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Claims 6
Claim · model-written summary
The BA, IBA and TDZ combination was best for shoot induction, giving 3.82 ± 0.18 shoots per explant.
- Taxon
- Jatropha curcas L.
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Treatment
- 1.5 mgL-1 benzyl adenine (BA) + 0.05 mgL-1 indole-3-butyric acid (IBA) + 0.5 mgL-1 thidiazuron (TDZ)e0
- Outcome
- shoot inductione0
- Direction
- beste0
- Value
- 3.82 ± 0.18 shoots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
t0“Jatropha curcas L.”
e0“1.5 mgL-1 benzyl adenine (BA) + 0.05 mgL-1 indole-3-butyric acid (IBA) + 0.5 mgL-1 thidiazuron (TDZ) turned out to be the best for shoot induction (3.82 ± 0.18 shoots/explant)”
Claim · model-written summary
Adding 25 mgL-1 each of glutamine and adenine sulfate was most effective for shoot production, yielding 9.09 ± 0.37 shoots per explant.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 25 mgL-1 each of glutamine and adenine sulfatee0
- Outcome
- shoots/explante0
- Direction
- most effectivee0
- Value
- 9.09 ± 0.37 shoots/explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas L.”
e0“25 mgL-1 each of glutamine and adenine sulfate was the most effective, resulting to 9.09 ± 0.37 shoots/explant and 93.0% regeneration frequency”
Claim · model-written summary
Adding 25 mgL-1 each of glutamine and adenine sulfate produced a 93.0% regeneration frequency.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 25 mgL-1 each of glutamine and adenine sulfatee0
- Outcome
- regeneration frequencye0
- Direction
- most effectivee0
- Value
- 93.0%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas L.”
e0“25 mgL-1 each of glutamine and adenine sulfate was the most effective, resulting to 9.09 ± 0.37 shoots/explant and 93.0% regeneration frequency”
Claim · model-written summary
Medium with 0.5 mgL-1 GA3 and 0.5 mgL-1 BA was best for shoot elongation, reaching 2.13 ± 0.18 cm.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 0.5 mgL-1 GA3 with 0.5 mgL-1 BAe0
- Outcome
- shoot elongatione0
- Direction
- beste0
- Value
- 2.13 ± 0.18 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas L.”
e0“0.5 mgL-1 GA3 with 0.5 mgL-1 BA were found to be the best for shoot elongation (2.13 ± 0.18 cm)”
Claim · model-written summary
Medium with 0.5 mgL-1 IBA yielded the highest rooting frequency of 40%.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- medium with 0.5 mgL-1 IBAe0
- Outcome
- frequency of roote0
- Direction
- higheste0
- Value
- 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas L.”
e0“The highest frequency of root (40%) was observed on the medium with 0.5 mgL-1 IBA”
Claim · model-written summary
The established procedures were proposed for mass propagation and genetic transformation of elite jatropha genotypes.
- Outcome
- mass propagation and genetic transformation of elite jatropha genotypese0
JatrophaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The established procedures will be useful for the mass propagation and genetic transformation of elite jatropha genotypes”
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glutamine · growth · Jatropha curcasResults agree
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.