Abstract
An efficient micropropagation protocol was developed for Jatropha curcas (L.) using nodal explants, and its genetic fidelity and was assessed using RAPD markers. Auxillary shoot buds were induced from seven–month–old nodal explants on MS medium supplemented with Kn, BAP, and TDZ along with AdS. A maximum number of shoot buds (6.2 ± 0.56) were induced on MS medium fortified with 21.6 μM BAP and 54.2 μM AdS. Shoot multiplication from the auxiliary buds was significantly enhanced (about 32.0 ± 0.01) on MS medium containing 0.4 μM IBA, 4.4 μM Kn, 21.6 μM BAP and 27.1 μM AdS. About 54% rooting was achieved on MS medium supplemented with 1.0 μM IBA. The well–rooted plantlets were established in green house with 91% survival frequency. A total of 15 RAPD primers produced in 925 distinct and reproducible bands and confirmed the genetic fidelity of the regenerated Jatropha curcas plants.
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Claims 5
Claim · model-written summary
Nodal explants produced a maximum of 6.2 ± 0.56 shoot buds under the stated BAP and AdS medium.
- Taxon
- Jatropha curcas (L.)
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Treatment
- MS medium fortified with 21.6 μM BAP and 54.2 μM AdSe0
- Outcome
- number of shoot budse0
- Direction
- maximume0
- Value
- 6.2 ± 0.56e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
t0“Jatropha curcas (L.)”
e0“A maximum number of shoot buds (6.2 ± 0.56) were induced on MS medium fortified with 21.6 μM BAP and 54.2 μM AdS.”
Claim · model-written summary
The IBA, Kn, BAP and AdS medium significantly enhanced shoot multiplication to about 32.0 ± 0.01.
- Taxon
- Jatropha curcas (L.)Jatropha curcast0
- Treatment
- MS medium containing 0.4 μM IBA, 4.4 μM Kn, 21.6 μM BAP and 27.1 μM AdSe0
- Outcome
- Shoot multiplicatione0
- Direction
- enhancede0
- Value
- about 32.0 ± 0.01e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas (L.)”
e0“Shoot multiplication from the auxiliary buds was significantly enhanced (about 32.0 ± 0.01) on MS medium containing 0.4 μM IBA, 4.4 μM Kn, 21.6 μM BAP and 27.1 μM AdS.”
Claim · model-written summary
MS medium with 1.0 μM IBA achieved about 54% rooting.
- Taxon
- Jatropha curcas (L.)Jatropha curcast0
- Treatment
- MS medium supplemented with 1.0 μM IBAe0
- Outcome
- rootinge0
- Value
- About 54%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas (L.)”
e0“About 54% rooting was achieved on MS medium supplemented with 1.0 μM IBA.”
Claim · model-written summary
Well-rooted plantlets survived greenhouse establishment at 91%.
- Taxon
- Jatropha curcas (L.)Jatropha curcast0
- Outcome
- survival frequencye0
- Value
- 91%e0
- Setting
- green housee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Jatropha curcas (L.)”
e0“The well–rooted plantlets were established in green house with 91% survival frequency.”
Claim · model-written summary
Fifteen RAPD primers yielded 925 reproducible bands and confirmed genetic fidelity of regenerated plants.
- Taxon
- Jatropha curcasJatropha curcase0
- Outcome
- genetic fidelitye0
- Value
- 925 distinct and reproducible bandse0
- Qualifier
- 15 RAPD primerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 15 RAPD primers produced in 925 distinct and reproducible bands and confirmed the genetic fidelity of the regenerated Jatropha curcas plants.”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.