Abstract
Plantlet regeneration in Jatropha curcas L. (Family: Euphorbiaceae), an energy shrub, has been studied from nodal and leaf explants on basal MS medium. The nodal explants were found superior to leaf explants. Higher shoot bud differentiation was observed on MS media supplemented with 8 mu M N-6-benzyladenine (BA; 6.2 +/- 0.83) from nodal explants in comparison to both 10 mu M kinetin (Kn; 2.8 +/- 0.45) from leaf explants and a combination of 6.0 mu M BA with 4.0 mu M Kn (5.0 +/- 0.71) from nodal explants. MS medium fortified with 8 mu M BA and 2 mu M IBA (indole butyric acid) was found most suitable for both callus mediated organogenesis and elongation of shoots. Addition of 45 mu M adenine sulphate, 15 mu M glutamine and 10 mu M proline to this optimized MS medium enhanced the number of multiple shoot proliferation (9.8 +/- 0.84) per explant and elongation at the end of 2nd wk. The elongated shoots were successfully rooted on half-strength MS medium with a prior incubation on MS medium with NAA (alpha-napthalene acetic acid) or IBA or a combination of both; 2 mu M IBA provided better response for rhizogenesis among them. Regenerated plantlets were successfully established in soil where 80 +/- 4% of them developed into morphologically normal and fertile plants. Forty RAPD decamer primers were used to assess genetic fidelity of regenerated plantlets along with the donor plant. Fourteen primers responded for amplification, generating 75 amplified products (160 to 2690 bp). The amplification pattern confirmed the genetic uniformity of the regenerated plantlets and substantiated the efficacy and suitability of this protocol for in vitro propagation of J. curcas, thereby favouring the economics of the cost of plant material and time factor.
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Claims 10
Claim · model-written summary
Nodal explants outperformed leaf explants for regeneration.
- Taxon
- Jatropha curcas L.
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Organ / stage
- nodal explantse0
- Outcome
- superiore0
- Direction
- superiore0
- Comparator
- leaf explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“The nodal explants were found superior to leaf explants”
t0“Jatropha curcas L.”
Claim · model-written summary
Nodal explants on 8 mu M BA had higher shoot bud differentiation, 6.2 +/- 0.83.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- nodal explantse0
- Treatment
- 8 mu M N-6-benzyladeninee0
- Outcome
- shoot bud differentiatione0
- Direction
- Highere0
- Value
- 6.2 +/- 0.83e0
- Comparator
- 10 mu M kinetine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher shoot bud differentiation was observed on MS media supplemented with 8 mu M N-6-benzyladenine (BA; 6.2 +/- 0.83) from nodal explants in comparison to both 10 mu M kinetin (Kn; 2.8 +/- 0.45) from leaf explants and a combination of 6.0 mu M BA with 4.0 mu M Kn (5.0 +/- 0.71) from nodal explants”
t0“Jatropha curcas L.”
Claim · model-written summary
MS medium with 8 mu M BA and 2 mu M IBA was most suitable for callus mediated organogenesis.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 8 mu M BA and 2 mu M IBAe0
- Outcome
- callus mediated organogenesise0
- Direction
- most suitablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium fortified with 8 mu M BA and 2 mu M IBA (indole butyric acid) was found most suitable for both callus mediated organogenesis and elongation of shoots”
t0“Jatropha curcas L.”
Claim · model-written summary
The same medium was most suitable for shoot elongation.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 8 mu M BA and 2 mu M IBAe0
- Outcome
- elongation of shootse0
- Direction
- most suitablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium fortified with 8 mu M BA and 2 mu M IBA (indole butyric acid) was found most suitable for both callus mediated organogenesis and elongation of shoots”
t0“Jatropha curcas L.”
Claim · model-written summary
Adenine sulphate, glutamine, and proline supplementation enhanced multiple shoot proliferation to 9.8 +/- 0.84 per explant.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 45 mu M adenine sulphate, 15 mu M glutamine and 10 mu M prolinee0
- Outcome
- number of multiple shoot proliferatione0
- Direction
- enhancede0
- Value
- 9.8 +/- 0.84e0
- Comparator
- this optimized MS mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 45 mu M adenine sulphate, 15 mu M glutamine and 10 mu M proline to this optimized MS medium enhanced the number of multiple shoot proliferation (9.8 +/- 0.84) per explant”
t0“Jatropha curcas L.”
Claim · model-written summary
The same supplementation enhanced shoot elongation by the end of week two.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 45 mu M adenine sulphate, 15 mu M glutamine and 10 mu M prolinee0
- Outcome
- elongatione0
- Direction
- enhancede0
- Qualifier
- at the end of 2nd wke0
- Comparator
- this optimized MS mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of 45 mu M adenine sulphate, 15 mu M glutamine and 10 mu M proline to this optimized MS medium enhanced the number of multiple shoot proliferation (9.8 +/- 0.84) per explant and elongation at the end of 2nd wk”
t0“Jatropha curcas L.”
Claim · model-written summary
2 mu M IBA gave the better response for rhizogenesis.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- 2 mu M IBAe0
- Outcome
- rhizogenesise0
- Direction
- bettere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2 mu M IBA provided better response for rhizogenesis among them”
t0“Jatropha curcas L.”
Claim · model-written summary
Regenerated plantlets established in soil, with 80 +/- 4% becoming morphologically normal and fertile.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- morphologically normal and fertile plantse0
- Value
- 80 +/- 4%e0
- Setting
- soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plantlets were successfully established in soil where 80 +/- 4% of them developed into morphologically normal and fertile plants”
t0“Jatropha curcas L.”
Claim · model-written summary
Fourteen RAPD primers amplified 75 products ranging from 160 to 2690 bp.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- amplified productse0
- Value
- 75 amplified productse0
- Qualifier
- 160 to 2690 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fourteen primers responded for amplification, generating 75 amplified products (160 to 2690 bp)”
t0“Jatropha curcas L.”
Claim · model-written summary
RAPD amplification patterns confirmed genetic uniformity of regenerated plantlets.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- genetic uniformity of the regenerated plantletse0
- Comparator
- donor plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Forty RAPD decamer primers were used to assess genetic fidelity of regenerated plantlets along with the donor plant. Fourteen primers responded for amplification, generating 75 amplified products (160 to 2690 bp). The amplification pattern confirmed the genetic uniformity of the regenerated plantlets”
t0“Jatropha curcas L.”
The same questions in other papers
proline · growth · Jatropha curcasResults agree
1 paper in the same direction as this paper
- positive
adenine · growth · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
sulfate · growth · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
glutamine · growth · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.