Abstract
Watermelon ( Citrullus vulgaris Schrad.) is a widely grown crop throughout the tropics and subtropics. In Mexico, it is an economically important crop. In vitro adventitious shoot regeneration of watermelon has been reported from shoot tip culture, leaf, hypocotyl, and cotyledons. Hence, the objective of this study was to evaluate in vitro plant regeneration from axillary buds of triploid watermelon. Axillary buds explants were prepared from shoot of commercial cultivar in field of 60 old day plants. Explants of 2 to 3 mm were incubated 2 weeks on Murashige and Skoog (MS) shoot regeneration medium containing 2.5 mg/L kinetin (KT) or indole-3-butyric acid (IBA), or gibberellic acid (GA 3 ), followed by 3 weeks on shoot elongation medium supplemented with different combinations of the same phytohormones. The percentage of explants (83% to 90%) that produced shoots, expansion in size of explant (0.81–1 cm) and shoot length (6 mm) were highest in MS medium containing KT or IBA. In the shoot elongation step, shoot length (0.9–1 cm) and leaves number (6–7) were highest in MS medium supplemented with 2.5 mg/L of KT or GA 3 and 0.2 mg/L IBA, but the better induction of roots in elongated shoot occurred on MS medium with 2.5 mg/L KT and 0.2 mg/L IBA. The results show that axillary buds from watermelon is an alternative for the micropropagation of this crop.
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Claims 7
Claim · model-written summary
KT or IBA medium produced shoots from 83–90% of explants.
- Taxon
- watermelon
Citrullus lanatust0 - Organ / stage
- shootse0
- Treatment
- MS medium containing KT or IBAe0
- Outcome
- percentage of explantse0
- Direction
- higheste0
- Value
- 83% to 90%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of explants (83% to 90%) that produced shoots, expansion in size of explant (0.81–1 cm) and shoot length (6 mm) were highest in MS medium containing KT or IBA.”
t0“Watermelon”
Claim · model-written summary
KT or IBA medium gave the greatest explant expansion, 0.81–1 cm.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MS medium containing KT or IBAe0
- Outcome
- expansion in size of explante0
- Direction
- higheste0
- Value
- 0.81–1 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of explants (83% to 90%) that produced shoots, expansion in size of explant (0.81–1 cm) and shoot length (6 mm) were highest in MS medium containing KT or IBA.”
t0“Watermelon”
Claim · model-written summary
KT or IBA medium yielded the greatest shoot length of 6 mm.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MS medium containing KT or IBAe0
- Outcome
- shoot lengthe0
- Direction
- higheste0
- Value
- 6 mme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of explants (83% to 90%) that produced shoots, expansion in size of explant (0.81–1 cm) and shoot length (6 mm) were highest in MS medium containing KT or IBA.”
t0“Watermelon”
Claim · model-written summary
Elongation medium with KT or GA3 plus IBA gave maximum shoot length of 0.9–1 cm.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MS medium supplemented with 2.5 mg/L of KT or GA 3 and 0.2 mg/L IBAe0
- Outcome
- shoot lengthe0
- Direction
- higheste0
- Value
- 0.9–1 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot length (0.9–1 cm) and leaves number (6–7) were highest in MS medium supplemented with 2.5 mg/L of KT or GA 3 and 0.2 mg/L IBA”
t0“Watermelon”
Claim · model-written summary
The same elongation medium gave the most leaves, 6–7.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MS medium supplemented with 2.5 mg/L of KT or GA 3 and 0.2 mg/L IBAe0
- Outcome
- leaves numbere0
- Direction
- higheste0
- Value
- 6–7e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot length (0.9–1 cm) and leaves number (6–7) were highest in MS medium supplemented with 2.5 mg/L of KT or GA 3 and 0.2 mg/L IBA”
t0“Watermelon”
Claim · model-written summary
Medium with KT and IBA best induced roots.
- Taxon
- watermelonCitrullus lanatust0
- Treatment
- MS medium with 2.5 mg/L KT and 0.2 mg/L IBAe0
- Outcome
- induction of rootse0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the better induction of roots in elongated shoot occurred on MS medium with 2.5 mg/L KT and 0.2 mg/L IBA.”
t0“Watermelon”
Claim · model-written summary
Axillary buds are proposed as an alternative watermelon micropropagation source.
- Taxon
- watermelonCitrullus lanatuse0
- Outcome
- axillary buds from watermelon is an alternative for the micropropagation of this crope0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“axillary buds from watermelon is an alternative for the micropropagation of this crop.”
The same questions in other papers
indole-3-butyric acid · growth · Citrullus lanatus (watermelon)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
kinetin · growth · Citrullus lanatus (watermelon)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Gibberellic acid · growth · Citrullus lanatus (watermelon)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →in vitro · growth · Citrullus lanatus (watermelon)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.