Abstract
Opportunities to increase the in vitro vegetative cloning efficiency of Pinus elliottii were explored. The hormonal dose response of axillary bud microshoot production was determined by varying the concentration of the treatment solution. The concentration of 222 uM benzyladenine produced the greatest number of shoots (33 and 68 total shoots from 15 explants each) as seen in two experiments and was consequently used as the constant concentration in experiments in which the relationship of length of time of explant exposure to the hormone was examined. The effect of multiple hormones on stimulation of axillary bud development as a function of micros hoot production varied between two unique genotypes of Pinus elliottii. Methods designed to stimulate the organogenic production of morphologically normal roots on the microshoots and explants were explored. Pulses of various hormones were tested as were the effects of bacteria grown on both agar and liquid media. Exposure of the hypocotylary explants to root·inducing bacterial isolates growo on the agar media provided the best results with up to 50% rooting of the explants.
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Claims 3
Claim · model-written summary
A 222 uM benzyladenine concentration produced the greatest number of shoots in two experiments.
- Taxon
- Pinus elliottii
.jpg?width=60)
Pinus elliottiie1 - Organ / stage
- shootse0
- Treatment
- 222 uM benzyladeninee0
- Outcome
- number of shootse0
- Direction
- greateste0
- Value
- 33 and 68 total shoots from 15 explants eache0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The concentration of 222 uM benzyladenine produced the greatest number of shoots (33 and 68 total shoots from 15 explants each) as seen in two experiments”
e1“in vitro vegetative cloning efficiency of Pinus elliottii”
Claim · model-written summary
The effect of multiple hormones on axillary bud microshoot production varied between two Pinus elliottii genotypes.
- Taxon
- Pinus elliottiiPinus elliottiie0
- Material
- two unique genotypes of Pinus elliottiie0
- Treatment
- multiple hormonese0
- Outcome
- micros hoot productione0
- Direction
- variede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of multiple hormones on stimulation of axillary bud development as a function of micros hoot production varied between two unique genotypes of Pinus elliottii”
Claim · model-written summary
Agar-grown root-inducing bacterial isolates gave the best hypocotyl explant rooting, up to 50%.
- Taxon
- Pinus elliottiiPinus elliottiie1
- Organ / stage
- hypocotylary explantse0
- Treatment
- Exposure of the hypocotylary explants to root·inducing bacterial isolates growo on the agar mediae0
- Interactor
- root·inducing bacterial isolatese0symbiont
- Outcome
- rooting of the explantse0
- Direction
- beste0
- Value
- up to 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure of the hypocotylary explants to root·inducing bacterial isolates growo on the agar media provided the best results with up to 50% rooting of the explants”
e1“in vitro vegetative cloning efficiency of Pinus elliottii”
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