Abstract
Slash pine (Pinus elliottii Engelm.) is an economically significant tree species valued for its timber and resin production. Establishing a somatic embryogenesis system is essential for the effective utilization of slash pine. This study aims to develop an optimized cultivation system for the long-term proliferation of embryogenic callus (EC) in slash pine. The study used the EC induced from immature zygotic embryos as materials to investigated the effects of plant growth regulators (PGRs), subculture cycles, and subculture durations on the EC proliferation coefficient and the number of somatic embryos (SE). Additionally, we monitored the changes in the proliferation coefficient, the number of SE, and physiological and biochemical indicators of EC during long-term proliferation. The EC proliferation coefficient reached its highest value (7.65) when the DCR medium was supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg/L 6-benzylaminopurine (6-BA). Refreshing the culture medium every 14 days, EC maintaining proliferation coefficient (4.05) and embryogenic potential for approximately three year (980 d), but the number of somatic embryos was low and significantly declined after 140 days.The additional supplementation of 1.0 mg/L 2- (1-naphthyl) acetic acid (NAA) to the original proliferation medium further enhanced SE induction, achieving a maximum of 143.25 SE per gram of EC. Under the optimal proliferation culture conditions, EC was subcultured for 392 days, with no significant differences observed in the proliferation coefficient and the number of SE. The results of correlation and path analyses between physiological indicators and proliferation coefficient indicate that the proliferation coefficient of EC is mainly related to the content of storage substances (starch and soluble sugars) and the activities of redox-related enzymes (POD and CAT). The optimized long-term proliferation culture system for EC in slash pine provides an efficient approach to enhance the production of EC and SE.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Supplemented DCR medium gave the highest embryogenic callus proliferation coefficient, 7.65.
- Taxon
- Slash pine
.jpg?width=60)
Pinus elliottiit0 - Organ / stage
- ECe0
- Treatment
- DCR medium was supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg/L 6-benzylaminopurine (6-BA)e0
- Outcome
- EC proliferation coefficiente0
- Direction
- higheste0
- Value
- 7.65e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The EC proliferation coefficient reached its highest value (7.65) when the DCR medium was supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg/L 6-benzylaminopurine (6-BA)”
t0“Slash pine”
Claim · model-written summary
Refreshing medium every 14 days maintained a proliferation coefficient of 4.05 for about three years.
- Taxon
- Slash pinePinus elliottiit0
- Organ / stage
- ECe0
- Treatment
- Refreshing the culture medium every 14 dayse0
- Outcome
- proliferation coefficiente0
- Direction
- maintaininge0
- Value
- 4.05e0
- Qualifier
- approximately three year (980 d)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Refreshing the culture medium every 14 days, EC maintaining proliferation coefficient (4.05) and embryogenic potential for approximately three year (980 d)”
t0“Slash pine”
Claim · model-written summary
With medium refreshed every 14 days, callus maintained embryogenic potential for approximately three years.
- Taxon
- Slash pinePinus elliottiit0
- Organ / stage
- ECe0
- Outcome
- embryogenic potentiale0
- Value
- approximately three year (980 d)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EC maintaining proliferation coefficient (4.05) and embryogenic potential for approximately three year (980 d)”
t0“Slash pine”
Claim · model-written summary
Somatic embryo number significantly declined after 140 days.
- Taxon
- Slash pinePinus elliottiit0
- Outcome
- number of somatic embryose0
- Direction
- declinede0
- Qualifier
- after 140 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of somatic embryos was low and significantly declined after 140 days”
t0“Slash pine”
Claim · model-written summary
Additional NAA enhanced somatic embryo induction to a maximum of 143.25 embryos per gram of callus.
- Taxon
- Slash pinePinus elliottiit0
- Treatment
- 1.0 mg/L 2- (1-naphthyl) acetic acid (NAA)e0
- Outcome
- SE inductione0
- Direction
- enhancede0
- Value
- 143.25 SE per gram of ECe0
- Comparator
- original proliferation mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The additional supplementation of 1.0 mg/L 2- (1-naphthyl) acetic acid (NAA) to the original proliferation medium further enhanced SE induction, achieving a maximum of 143.25 SE per gram of EC”
t0“Slash pine”
Claim · model-written summary
During 392 days of subculture under optimal conditions, proliferation coefficient did not differ significantly.
- Taxon
- Slash pinePinus elliottiit0
- Outcome
- proliferation coefficiente0
- Direction
- no significant differencese0
- Qualifier
- 392 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under the optimal proliferation culture conditions, EC was subcultured for 392 days, with no significant differences observed in the proliferation coefficient and the number of SE”
t0“Slash pine”
Claim · model-written summary
During 392 days of subculture under optimal conditions, somatic embryo number did not differ significantly.
- Taxon
- Slash pinePinus elliottiit0
- Outcome
- number of SEe0
- Direction
- no significant differencese0
- Qualifier
- 392 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under the optimal proliferation culture conditions, EC was subcultured for 392 days, with no significant differences observed in the proliferation coefficient and the number of SE”
t0“Slash pine”
Claim · model-written summary
Callus proliferation coefficient was mainly related to starch and soluble sugar content.
- Taxon
- Slash pinePinus elliottiit0
- Compound
- starch and soluble sugarse0
- Outcome
- proliferation coefficient of ECe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proliferation coefficient of EC is mainly related to the content of storage substances (starch and soluble sugars)”
t0“Slash pine”
Claim · model-written summary
Callus proliferation coefficient was mainly related to POD and CAT activities.
- Taxon
- Slash pinePinus elliottiit0
- Gene
- POD and CATe0
- Outcome
- proliferation coefficient of ECe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proliferation coefficient of EC is mainly related to the content of storage substances (starch and soluble sugars) and the activities of redox-related enzymes (POD and CAT)”
t0“Slash pine”
The same questions in other papers
2,4-D · growth · Pinus elliottii (slash pine)Results agree
1 paper in the same direction as this paper
- positive
N-benzyladenine · growth · Pinus elliottii (slash pine)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Pinus elliottii (slash pine)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.