Abstract
In vitro plant regeneration from callus induced from embryos of mature seeds of 4 Australian varieties of rice was studied. Observations of callus induction on MS and N6 media indicated that MS medium supplemented with 0.5–2 mg/L of 2,4-D is suitable for callus formation from the varieties tested. Comparison of shoot initiation on medium containing BAP, BAP + NAA, and TDZ + NAA indicated that these varieties prefer BAP + NAA or TDZ + NAA in the shoot initiation medium. Partial desiccation, resulting in up to 20% loss of fresh weight of callus, significantly increased the regeneration frequency of the 4 rice varieties tested. The varieties showed varied response to number of shoots produced per callus. Regenerated shoots were rooted on plant growth regulator free medium. The plants regenerated were phenotypically normal and fertile. Our study showed that callus derived from mature embryos of these rice varieties are amenable to multiple shoot formation, and could be used for genetic transformation studies.
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Claims 8
Claim · model-written summary
MS medium with 0.5–2 mg/L 2,4-D was suitable for callus formation.
- Taxon
- rice
Oryza sativae1 - Treatment
- MS medium supplemented with 0.5–2 mg/L of 2,4-De0
- Outcome
- callus formatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 0.5–2 mg/L of 2,4-D is suitable for callus formation from the varieties tested”
e1“varieties of rice”
Claim · model-written summary
The varieties preferred BAP + NAA for shoot initiation.
- Taxon
- riceOryza sativae1
- Treatment
- BAP + NAAe0
- Outcome
- shoot initiatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these varieties prefer BAP + NAA or TDZ + NAA in the shoot initiation medium”
e1“varieties of rice”
Claim · model-written summary
The varieties preferred TDZ + NAA for shoot initiation.
- Taxon
- riceOryza sativae1
- Treatment
- TDZ + NAAe0
- Outcome
- shoot initiatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these varieties prefer BAP + NAA or TDZ + NAA in the shoot initiation medium”
e1“varieties of rice”
Claim · model-written summary
Partial desiccation significantly raised regeneration frequency.
- Taxon
- riceOryza sativae0
- Treatment
- Partial desiccatione0
- Outcome
- regeneration frequencye0
- Direction
- increasede0
- Value
- up to 20%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Partial desiccation, resulting in up to 20% loss of fresh weight of callus, significantly increased the regeneration frequency of the 4 rice varieties tested”
Claim · model-written summary
Varieties differed in shoots produced per callus.
- Taxon
- riceOryza sativae1
- Outcome
- number of shoots produced per calluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The varieties showed varied response to number of shoots produced per callus”
e1“varieties of rice”
Claim · model-written summary
Regenerated shoots rooted on regulator-free medium.
- Taxon
- riceOryza sativae1
- Treatment
- plant growth regulator free mediume0
- Outcome
- Regenerated shoots were rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated shoots were rooted on plant growth regulator free medium”
e1“varieties of rice”
Claim · model-written summary
Regenerated plants were phenotypically normal and fertile.
- Taxon
- riceOryza sativae1
- Outcome
- phenotypically normal and fertilee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plants regenerated were phenotypically normal and fertile”
e1“varieties of rice”
Claim · model-written summary
Mature-embryo callus was amenable to multiple shoot formation and proposed for transformation studies.
- Taxon
- riceOryza sativae0
- Outcome
- multiple shoot formatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus derived from mature embryos of these rice varieties are amenable to multiple shoot formation, and could be used for genetic transformation studies”
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