Abstract
Embryogenic calli from mature seeds of four indica rice genotypes were used to observe their regeneration potentiality and establish a suitable in vitro plantlet regeneration protocol. MS medium supplemented with different phytohormone combinations were used to observe the callus induction ability of the explant. The highest callus induction (73.19%), biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L -1 2,4-D over all the genotypes and MS medium supplemented with 1.5 mg L -1 2,4-D was the best over all the treatments (66.83%). Among the phytohormone combinations, MS + 8 mg L -1 Kinetin + 0.5 mg L -1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm). Among the genotypes, Binadhan-6 was highly responsive to shoot regeneration (55.83%). The best root formation from regenerants (87.889%), maximum number of roots per plant ( In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively. This callus induction and in vitro regeneration protocol will be widely applicable for the tissue culture of indica rice.
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Claims 11
Claim · model-written summary
Binadhan-6 had the highest callus induction, 73.19%, with the specified 2,4-D medium.
- Taxon
- indica rice
Oryza sativa Indica Groupt0 - Material
- Binadhan-6e0
- Treatment
- MS medium supplemented with 1.0 mg L -1 2,4-De0
- Outcome
- callus inductione0
- Direction
- higheste0
- Value
- 73.19%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest callus induction (73.19%), biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L -1 2,4-D”
t0“indica rice genotypes”
Claim · model-written summary
Binadhan-6 had the biggest callus, 3.133mm, with the specified 2,4-D medium.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Binadhan-6e0
- Treatment
- MS medium supplemented with 1.0 mg L -1 2,4-De0
- Outcome
- size of calluse0
- Direction
- biggeste0
- Value
- 3.133mme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L -1 2,4-D”
t0“indica rice genotypes”
Claim · model-written summary
Binadhan-6 had higher callus weight, 0.7167g, with the specified 2,4-D medium.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Binadhan-6e0
- Treatment
- MS medium supplemented with 1.0 mg L -1 2,4-De0
- Outcome
- callus weighte0
- Direction
- highere0
- Value
- 0.7167ge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L -1 2,4-D”
t0“indica rice genotypes”
Claim · model-written summary
The medium with 1.5 mg L -1 2,4-D gave the best callus induction across treatments, 66.83%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- MS medium supplemented with 1.5 mg L -1 2,4-De0
- Outcome
- callus induction abilitye1
- Direction
- beste0
- Value
- 66.83%e0
- Comparator
- all the treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1.5 mg L -1 2,4-D was the best over all the treatments (66.83%)”
e1“MS medium supplemented with different phytohormone combinations were used to observe the callus induction ability of the explant”
t0“indica rice genotypes”
Claim · model-written summary
The kinetin and NAA combination gave the highest shoot regeneration, 50.67%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- MS + 8 mg L -1 Kinetin + 0.5 mg L -1 NAAe0
- Outcome
- shoot regeneratione0
- Direction
- higheste0
- Value
- 50.67%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS + 8 mg L -1 Kinetin + 0.5 mg L -1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm)”
t0“indica rice genotypes”
Claim · model-written summary
The kinetin and NAA combination gave shoot length of 13.7cm, reported as highest.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- MS + 8 mg L -1 Kinetin + 0.5 mg L -1 NAAe0
- Outcome
- shoot lengthe0
- Direction
- higheste0
- Value
- 13.7cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS + 8 mg L -1 Kinetin + 0.5 mg L -1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm)”
t0“indica rice genotypes”
Claim · model-written summary
Binadhan-6 was highly responsive to shoot regeneration, at 55.83%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Binadhan-6e0
- Outcome
- shoot regeneratione0
- Direction
- highly responsivee0
- Value
- 55.83%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the genotypes, Binadhan-6 was highly responsive to shoot regeneration (55.83%)”
t0“indica rice genotypes”
Claim · model-written summary
The best root formation from regenerants was 87.889%; the source sentence is truncated.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- root formation from regenerantse0
- Direction
- beste0
- Value
- 87.889%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best root formation from regenerants (87.889%)”
t0“indica rice genotypes”
Claim · model-written summary
Binadhan-6 had the highest plantlet survival rate in pots, 91.30%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Binadhan-6e0
- Outcome
- survival rate of the plantlete0
- Direction
- higheste0
- Value
- 91.30%e0
- Setting
- pote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively”
t0“indica rice genotypes”
Claim · model-written summary
Binadhan-6 had the highest plantlet survival rate in soil, 85%.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- Binadhan-6e0
- Outcome
- survival rate of the plantlete0
- Direction
- higheste0
- Value
- 85%e0
- Setting
- soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively”
t0“indica rice genotypes”
Claim · model-written summary
The authors considered the callus induction and regeneration protocol widely applicable to indica rice tissue culture.
- Taxon
- indica riceOryza sativa Indica Groupe0
- Outcome
- widely applicable for the tissue culture of indica ricee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This callus induction and in vitro regeneration protocol will be widely applicable for the tissue culture of indica rice”
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