Abstract
Expression and physiological effects of the root-inducing rolB gene of Agrobacterium rhizogenes T-DNA were studied simultaneously in tobacco (Nicotiana tabacum) mesophyll protoplasts. The kinetic study of the expression of rolB mRNA following exogenous auxin application showed that auxin transiently stimulated rolB expression, with mRNA levels starting to accumulate 6 to 9 h after auxin was supplied and increasing 300-fold after 12 to 18 h. The parallel study of the auxin sensitivity of rolB-transformed protoplasts, as assayed by their electrical response to the hormone, showed that the auxin treatment generated an increase in sensitivity by a factor of up to 100,000, whereas in untransformed protoplasts the same auxin treatment induced an increase in auxin sensitivity that never exceeded 30- to 50-fold. This reflects a strong cooperative effect of auxin and rolB in transformed protoplasts. Surprisingly, the maximal increase in sensitivity was observed several hours before the maximal accumulation of rolB mRNA, suggesting that the dramatic control of auxin sensitivity by auxin in rolB-transformed protoplasts requires only low levels of rolB expression. Antibodies directed against ZmER-abp1, the major auxin-binding protein from maize, differentially altered the auxin sensitivity of the electrical response of rolB-transformed and normal protoplasts. This suggests that alterations of the auxin reception-transduction pathway at the plasma membrane of rolB-transformed protoplasts may account for their increased auxin sensitivity.
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Claims 5
Claim · model-written summary
Auxin transiently stimulated rolB mRNA expression, reaching 300-fold after 12 to 18 hours.
- Taxon
- tobacco (Nicotiana tabacum)
Nicotiana tabacume1 - Organ / stage
- mesophyll protoplastse1
- Treatment
- auxine0
- Gene
- rolBe0
- Outcome
- rolB expressione0
- Direction
- increasinge0
- Value
- 300-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin transiently stimulated rolB expression, with mRNA levels starting to accumulate 6 to 9 h after auxin was supplied and increasing 300-fold after 12 to 18 h”
e1“tobacco (Nicotiana tabacum) mesophyll protoplasts”
Claim · model-written summary
Auxin treatment increased sensitivity by up to 100,000-fold in rolB-transformed protoplasts.
- Taxon
- tobacco (Nicotiana tabacum)Nicotiana tabacume1
- Material
- rolB-transformed protoplastse2
- Treatment
- auxin treatmente0
- Outcome
- sensitivitye0
- Direction
- increasee0
- Value
- a factor of up to 100,000e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the auxin treatment generated an increase in sensitivity by a factor of up to 100,000”
e1“tobacco (Nicotiana tabacum) mesophyll protoplasts”
e2“auxin sensitivity of rolB-transformed protoplasts”
Claim · model-written summary
The same auxin treatment raised sensitivity in untransformed protoplasts by at most 30- to 50-fold.
- Taxon
- tobacco (Nicotiana tabacum)Nicotiana tabacume1
- Material
- untransformed protoplastse0
- Treatment
- auxin treatmente0
- Outcome
- auxin sensitivitye0
- Direction
- increasee0
- Value
- 30- to 50-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in untransformed protoplasts the same auxin treatment induced an increase in auxin sensitivity that never exceeded 30- to 50-fold”
e1“tobacco (Nicotiana tabacum) mesophyll protoplasts”
Claim · model-written summary
Maximum sensitivity preceded maximum rolB mRNA accumulation by several hours.
- Taxon
- tobacco (Nicotiana tabacum)Nicotiana tabacume1
- Gene
- rolBe0
- Outcome
- maximal increase in sensitivitye0
- Qualifier
- several hours before the maximal accumulation of rolB mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximal increase in sensitivity was observed several hours before the maximal accumulation of rolB mRNA”
e1“tobacco (Nicotiana tabacum) mesophyll protoplasts”
Claim · model-written summary
Antibodies against ZmER-abp1 differentially altered auxin sensitivity in transformed and normal protoplasts.
- Taxon
- tobacco (Nicotiana tabacum)Nicotiana tabacume1
- Treatment
- Antibodies directed against ZmER-abp1e0
- Gene
- ZmER-abp1e0
- Outcome
- auxin sensitivity of the electrical responsee0
- Comparator
- normal protoplastse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antibodies directed against ZmER-abp1, the major auxin-binding protein from maize, differentially altered the auxin sensitivity of the electrical response of rolB-transformed and normal protoplasts”
e1“tobacco (Nicotiana tabacum) mesophyll protoplasts”
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.