Abstract
포도 Campbell Early을 공시하여 기내 배양에 의한 대량 증식과 건전묘의 생산시 영향을 미치는 DIF조건과 cytokinin의 종류 (BA, TDZ, zeatin, 2iP, kinetin)및 농도의 최적 조건을 구명 하고자 수행한 실험의 결과는 다음과 같다. 신초생장은 0(25/2$^{\circ}C$)및 ＋7DIF(25/18$^{\circ}C$) 처리에서 좋았고. -7DIF(25/18$^{\circ}C$)에서는 생장이 전반적으로 나빴다. DIF 처리와 관계없이 BA와 TDZ 처리에서는 생장이 나빴으며 -7DIF 처리는 생육이 억제되었다. 분지수와 총절편체수도 모든 DIF 처리에서 신초 생장이 적었던 BA와 TDZ 처리에서 분지가 많았고, 총절편체수도 BA와 TDZ 처리에서 많은 것으로 나타났다. DIF와 상관없이 모든 zeatin과 kinetin 처리는 신초형성 효과는 없었고 오히려 억제적으로 작용하였다. 모든 DIF 처리구에서 주온과 야온에 상관없이 대조구와 같거나 약간 낮았다. 0DIF 처리에서 절간수가 증가하였으며 - 및 ＋7DIF 처리에서는 모든 시토키닌 처리구에서 0DIF 처리보다 낮은 결과를 보였다. 생체중은 0DIF 처리에서만 효과가 좋았으며 모든 DIF 처리의 대조구도 생체중이 상당히 낮았다. DIF 처리는 포도 기내배양의 생체중에 영향을 미쳤는데, 이는 신초 생장과 관계가 깊은 것으로 추정된다. Effects of difference between day and night temperatures (DIF) and cytokinins on in vitro growth of 'Campbell Early' grape plantlets were investigated, Shoot growth was suppressed by -7DIF (18/$25^{\circ}C$). Effects of BA and TDZ were not affected by DIF. 0DIF (25/$25^{\circ}C$) enhanced the growth with increasing cytokinin level. Kinetin at 10 $\mu$M was the most effective for shoot growth with 87 mm among cytokinins used except for control. Zeatin and kinetin each at 5 $\mu$M significantly increased shoot growth to respective 162 and 110 mm. In 0DIF, two-fold increase of 162 mm was obtained by 5 $\mu$M zeatin. In control, no further shoot proliferation was observed regardless of DIF. In 0 and ＋7DIF (25/18$^{\circ}C$), 4 shoots were observed with 10 $\mu$M BA. Zeatin, 2iP and kinetin resulted in poor shoot proliferation while BA at 10 $\mu$M resulted in profuse branching in all DIFs.
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Claims 8
Claim · model-written summary
Shoot growth of grape plantlets was suppressed at -7DIF.
Not reportedComparator
Verbatim
e0“grape plantlets were investigated”
e1“Shoot growth was suppressed by -7DIF”
Claim · model-written summary
Effects of BA and TDZ on grape plantlets were not affected by DIF.
Verbatim
e0“grape plantlets were investigated”
e2“Effects of BA and TDZ were not affected by DIF”
Claim · model-written summary
Growth of grape plantlets was enhanced with increasing cytokinin level at 0DIF.
Not reportedComparator
Verbatim
e0“grape plantlets were investigated”
e3“enhanced the growth with increasing cytokinin level”
Claim · model-written summary
Kinetin at 10 µM was the most effective cytokinin for shoot growth of grape plantlets, with 87 mm.
Verbatim
e0“grape plantlets were investigated”
e4“Kinetin at 10 $\mu$M was the most effective for shoot growth with 87 mm among cytokinins used except for control”
Claim · model-written summary
Zeatin at 5 µM significantly increased shoot growth of grape plantlets to 162 mm.
Not reportedComparator
Verbatim
e0“grape plantlets were investigated”
e5“Zeatin and kinetin each at 5 $\mu$M significantly increased shoot growth to respective 162 and 110 mm”
Claim · model-written summary
Kinetin at 5 µM significantly increased shoot growth of grape plantlets to 110 mm.
Not reportedComparator
Verbatim
e0“grape plantlets were investigated”
e5“Zeatin and kinetin each at 5 $\mu$M significantly increased shoot growth to respective 162 and 110 mm”
Claim · model-written summary
BA at 10 µM resulted in profuse branching of grape plantlets in all DIF treatments.
Not reportedComparator
Verbatim
e0“grape plantlets were investigated”
e6“BA at 10 $\mu$M resulted in profuse branching in all DIFs”
Claim · model-written summary
Zeatin, 2iP and kinetin resulted in poor shoot proliferation of grape plantlets.
- Taxon
- grapee0
- Treatment
- Zeatin, 2iP and kinetine7
- Outcome
- shoot proliferatione7
- Direction
- decreaseinferrede7
Not reportedComparator
Verbatim
e0“grape plantlets were investigated”
e7“Zeatin, 2iP and kinetin resulted in poor shoot proliferation”
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