Abstract
포도 두 품종 ('Cabernet Sauvignon', 'Campbell Early')을 공시하여 기내 생장에 영향을 미치는 cytokinin의 종류 (BA, TDZ, zeatin. 2iP, kinetin) 및 농도의 최적 조건을 구명 하고자 수행한 실험의 결과는 다음과 같다. Cytokinin의 단용 처리에서 신초 생장은 두 품종이 비슷한 경향을 보여 대조구가 가장 좋았다. 분지수는 BA 10 $\mu$M 처리구에서 가장 많았고 TDZ 처리구가 그 다음이었으며, 그 외 처리구에서는 거의 분지되지 않았다. BA 10 $\mu$M 처리에서 총절편체수도 가장 많았으며, TDZ 처리가 그 뒤를 이었다. TDZ 처리에서 생체중이월등히 높았으며 캘러스 형성도 많이 되었으나 신초 생장은 좋지 않았다 'Cabernet Sauvignon'은 zeatin 10 $\mu$M 처리에서 발근되었고 'Campbell Early'는 2iP 10 $\mu$M 처리에서 많이 되었으며, BA와 TDZ 처리에서는 발근되지 않았다. Cytokinin 혼용 처리는 두 품종 모두 단용 처리에서와 마찬가지로 대조구에서 신초생장이 가장 좋았다. 단용 처리에서는 분지되지 않았던 zeatin과 2iP, kinetin에 TDZ를 혼용 처리했을 때 분지가 잘 되었으나, BA나 TDZ 단용 처리보다는 분지수가 적었다. 총절편체수와 생체중도 TDZ 처리구에서 좋은 효과를 보였으며 대조구보다 최고 10배나 높았다. 발근은 대조구에서만 되었으나,'Campbell Early'에서 예외적으로 zeatin과 kinetin 혼용 처리구에서 발근이 되었다. Effect of cytokinins (BA, TDZ, zeatin, 2iP, and kinetin) applied either singly or in combination on in vitro growth of two grape cultivars ('Cabernet Sauvignon' and 'Campbell Early') was investigated as a serial work for mass production of grapevine nursery stocks. In single treatment, shoot growth of two cultivars was most favorable in control. Shoot proliferation was satisfactory with 10 $\mu$M BA regardless of cultivars and cytokinin combinations, followed by TDZ. Other treatments resulted in very poor or no branching. Total explants ready for subculture produced by 10 $\mu$M BA outnumbered those by other treatments. TDZ was also effective. TDZ significantly increased the fresh weight and callus formation while shoot growth was unsatisfactory. Shoot growth response of two cultivars in combined treatments was also most favorable in control as was in single treatments. When TDZ was combined with zeatin, 2iP, and kinetin which failed to induce branching, proliferous branching was induced though the shoot number was behind that of single treatments of BA and TDZ. TDZ was very effective for total number of explants and fresh weight, showing 10-fold increase.
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Claims 10
Claim · model-written summary
Untreated controls had the most favorable shoot growth under single cytokinin treatments.
Verbatim
e0“In single treatment, shoot growth of two cultivars was most favorable in control.”
t0“Grapes”
Claim · model-written summary
BA at 10 μM gave satisfactory shoot proliferation in both cultivars.
Not reportedComparator
Verbatim
e0“Shoot proliferation was satisfactory with 10 $\mu$M BA regardless of cultivars and cytokinin combinations, followed by TDZ.”
t0“Grapes”
Claim · model-written summary
TDZ followed BA for shoot proliferation.
Verbatim
e0“Shoot proliferation was satisfactory with 10 $\mu$M BA regardless of cultivars and cytokinin combinations, followed by TDZ.”
t0“Grapes”
Claim · model-written summary
Other cytokinin treatments produced little or no branching.
Not reportedComparator
Verbatim
e0“Other treatments resulted in very poor or no branching.”
t0“Grapes”
Claim · model-written summary
BA at 10 μM produced more explants ready for subculture than other treatments.
Verbatim
e0“Total explants ready for subculture produced by 10 $\mu$M BA outnumbered those by other treatments.”
t0“Grapes”
Claim · model-written summary
TDZ significantly increased fresh weight and callus formation.
Not reportedComparator
Verbatim
e0“TDZ significantly increased the fresh weight and callus formation while shoot growth was unsatisfactory.”
t0“Grapes”
Claim · model-written summary
Under TDZ, shoot growth was unsatisfactory.
Verbatim
e0“TDZ significantly increased the fresh weight and callus formation while shoot growth was unsatisfactory.”
t0“Grapes”
Claim · model-written summary
Controls also had the most favorable shoot growth under combined cytokinin treatments.
Verbatim
e0“Shoot growth response of two cultivars in combined treatments was also most favorable in control as was in single treatments.”
t0“Grapes”
Claim · model-written summary
TDZ combined with zeatin, 2iP or kinetin induced branching, below single BA and TDZ treatments.
Verbatim
e0“When TDZ was combined with zeatin, 2iP, and kinetin which failed to induce branching, proliferous branching was induced though the shoot number was behind that of single treatments of BA and TDZ.”
t0“Grapes”
Claim · model-written summary
TDZ increased explant number and fresh weight up to tenfold.
- Taxon
- Grapest0
- Treatment
- TDZe0
- Outcome
- total number of explants and fresh weighte0
- Direction
- increasee0
- Value
- 10-folde0
Not reportedComparator
Verbatim
e0“TDZ was very effective for total number of explants and fresh weight, showing 10-fold increase.”
t0“Grapes”
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