Abstract
A 712 bp partial cDNA clone (czbp- 1) of the citrus vascular zinc binding protein (CVZBP) was isolated using reverse transcriptase polymerase chain reaction (RT-PCR). The deduced amino acid sequence of czbp-1 was identical to the N-terminal amino acid sequence for the CVZBP. Czbp- 1 had a 549 bp open reading frame and two putative polyadenylation sites, +20 bp and +103 bp relative to the poly-A tail. The deduced amino acid sequence had identity with members of the Kunitz soybean proteinase inhibitor (KSPI) family. Many members of this family are present in high concentrations in storage organs such as seeds and tubers, increase in response to abiotic stress, and are considered defense or stress response proteins. The CVZBP did not appear to fit in this category. Unlike many members of the KSPI family CVZBP was not detected in citrus seeds and protein levels decreased in response to wounding. Transcript also decreased in response to osmotic stress; a similar result previously was reported for CVZBP protein levels. Accumulation of CVZBP and its transcript increased in Zn deficient citrus seedlings compared to those receiving sufficient levels of Zn, indicating that Zn nutrition can modulate CVZBP expression. Recombinant CVZBP was produced and used to determine the capacity of this protein to inhibit several types of proteinases. The CVZBP inhibited the cysteine proteinase, papain, but not the serine proteinases, trypsin and chymotrypsin. CVZBP protein was immunolocalized primarily to the xylem parenchyma in vascular tissue of citrus midribs. Based on these results it is possible that the CVZBP has a function in vascular differentiation. Cysteine proteinases were identified in developing tracheary elements in Zinnia cell cultures. Addition of inhibitors of cysteine proteinase to these cultures prior to secondary cell wall deposition prevents differentiation of the cells into tracheary elements. Perhaps cysteine proteinase inhibitors, such as the CVZBP, in the xylem, contribute to timing of tracheary element differentiation and determination.
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Claims 16
Claim · model-written summary
A 712 bp partial cDNA clone of citrus CVZBP was isolated.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“A 712 bp partial cDNA clone (czbp- 1) of the citrus vascular zinc binding protein (CVZBP) was isolated”
Claim · model-written summary
The deduced czbp-1 amino acid sequence matched the CVZBP N terminus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“The deduced amino acid sequence of czbp-1 was identical to the N-terminal amino acid sequence for the CVZBP”
Claim · model-written summary
Czbp-1 has a 549 bp open reading frame.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“Czbp- 1 had a 549 bp open reading frame”
Claim · model-written summary
Czbp-1 has two putative polyadenylation sites at +20 bp and +103 bp relative to the poly-A tail.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“two putative polyadenylation sites, +20 bp and +103 bp relative to the poly-A tail”
e1“Czbp- 1 had a 549 bp open reading frame”
Claim · model-written summary
The deduced sequence has identity with the KSPI family.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“The deduced amino acid sequence had identity with members of the Kunitz soybean proteinase inhibitor (KSPI) family”
e1“The deduced amino acid sequence of czbp-1 was identical to the N-terminal amino acid sequence for the CVZBP”
Claim · model-written summary
CVZBP was not detected in citrus seeds.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CVZBP was not detected in citrus seeds”
Claim · model-written summary
CVZBP protein levels decreased after wounding.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“protein levels decreased in response to wounding”
e1“CVZBP was not detected in citrus seeds”
Claim · model-written summary
CVZBP transcript decreased under osmotic stress.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“Transcript also decreased in response to osmotic stress”
e1“CVZBP was not detected in citrus seeds”
Claim · model-written summary
CVZBP protein and transcript accumulated under zinc deficiency versus sufficient zinc.
- Treatment
- Zn deficiente0
- Gene
- CVZBPe0
- Outcome
- Accumulation of CVZBP and its transcripte0
- Direction
- increasede0
- Comparator
- sufficient levels of Zne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of CVZBP and its transcript increased in Zn deficient citrus seedlings compared to those receiving sufficient levels of Zn”
Claim · model-written summary
CVZBP inhibited papain.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“The CVZBP inhibited the cysteine proteinase, papain”
Claim · model-written summary
CVZBP did not inhibit trypsin or chymotrypsin.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“but not the serine proteinases, trypsin and chymotrypsin”
e1“The CVZBP inhibited the cysteine proteinase, papain”
Claim · model-written summary
CVZBP protein localized primarily to xylem parenchyma of citrus midribs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CVZBP protein was immunolocalized primarily to the xylem parenchyma in vascular tissue of citrus midribs”
Claim · model-written summary
CVZBP may function in vascular differentiation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“it is possible that the CVZBP has a function in vascular differentiation”
Claim · model-written summary
Cysteine proteinases were identified in developing Zinnia tracheary elements.
ZinniaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cysteine proteinases were identified in developing tracheary elements in Zinnia cell cultures”
Claim · model-written summary
Cysteine proteinase inhibitors prevent tracheary element differentiation in Zinnia cultures.
- Treatment
- inhibitors of cysteine proteinasee0
- Outcome
- differentiation of the cells into tracheary elementse0
- Direction
- preventse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of inhibitors of cysteine proteinase to these cultures prior to secondary cell wall deposition prevents differentiation of the cells into tracheary elements”
e1“Zinnia cell cultures”
Claim · model-written summary
CVZBP in xylem may contribute to timing and determination of tracheary differentiation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus”
e0“Perhaps cysteine proteinase inhibitors, such as the CVZBP, in the xylem, contribute to timing of tracheary element differentiation and determination”
The same questions in other papers
drought · molecular: expression · CitrusPositive and negative results
3 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →mechanical · molecular: expression · CitrusResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.