Paper · 2026 · Mycorrhiza

Inoculation is more effective for main crops than for preceding cover crops and does not affect the arbuscular mycorrhizal community in no-till vegetable systems

Clarissa Castoldi Facco; Emanuela Pille da Silva; Juliana Aparecida dos Santos; et al.

6
claims
7
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

Arbuscular mycorrhizal fungi (AMF) establish symbioses with most terrestrial plants, enhancing nutrient uptake, stress tolerance, and soil quality. We evaluated the use of Rhizophagus irregularis-based inoculants in cover crops and subsequent commercial crops in a no-till system and assessed their effects on soil biological attributes and crop yield. Three on-farm field experiments in different locations were conducted with common bean, okra, and corn cultivated after the winter cover crops. The assays were arranged in a randomized complete block design with a split-plot scheme and four replicates, with cover crop inoculation (black oats) as the main factor and crop inoculation in the subplot. The treatments included (i) inoculation in the cover crop and no inoculation in the main crop; (ii) no inoculation in the cover crop and inoculation in the main crop; (iii) inoculation in both crops; and (iv) no inoculation in either crop. Soil acid phosphatase activity, fluorescein diacetate hydrolysis, AMF spore density, and crop yield were measured at the end of each cycle, and the AMF community composition was characterized. The AMF inoculant increased the soil quality indicators (acid phosphatase activity and fluorescein diacetate hydrolysis). Inoculation of the cover crops did not replace the beneficial effects of direct inoculation on the yields of beans, okra, or corn. The results of the experiments in locations with different soils revealed that, overall, direct crop inoculation increased the yields of bean, okra, and corn by 30%, 33%, and 19%, respectively, and intensified soil enzyme activity, with no detectable effects on soil AMF community diversity or structure.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The AMF inoculant increased the soil quality indicators acid phosphatase activity and fluorescein diacetate hydrolysis.

Treatment
AMF inoculante1
Interactor
AMFe1symbiont
Outcome
acid phosphatase activity and fluorescein diacetate hydrolysise1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗
Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“common bean, okra, and corn”

✓ located verbatim in the source · abstract, characters 428–455 · show in text ↑
e1

“The AMF inoculant increased the soil quality indicators (acid phosphatase activity and fluorescein diacetate hydrolysis)”

✓ located verbatim in the source · abstract, characters 1136–1256 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Inoculation of the cover crops did not replace the beneficial effects of direct inoculation on the yields of beans, okra, or corn.

Taxon
beans, okra, or corne1
Treatment
Inoculation of the cover cropse1
Outcome
yieldse1
Direction
did not replacee1
Comparator
direct inoculatione1

Verbatim

e1

“Inoculation of the cover crops did not replace the beneficial effects of direct inoculation on the yields of beans, okra, or corn”

✓ located verbatim in the source · abstract, characters 1258–1387 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Direct crop inoculation increased bean yield by 30%.

Taxon
beane1
Treatment
direct crop inoculatione1
Outcome
yieldse1
Direction
increasede1
Value
30%e1

Not reportedComparator

Verbatim

e1

“direct crop inoculation increased the yields of bean, okra, and corn by 30%, 33%, and 19%, respectively”

✓ located verbatim in the source · abstract, characters 1478–1581 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Direct crop inoculation increased okra yield by 33%.

Treatment
direct crop inoculatione1
Outcome
yieldse1
Direction
increasede1
Value
33%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Abelmoschus esculentus
Abelmoschus esculentus · TaxonKristine Paulus from New York, United States · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e1

“direct crop inoculation increased the yields of bean, okra, and corn by 30%, 33%, and 19%, respectively”

✓ located verbatim in the source · abstract, characters 1478–1581 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Direct crop inoculation increased corn yield by 19%.

Treatment
direct crop inoculatione1
Outcome
yieldse1
Direction
increasede1
Value
19%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“direct crop inoculation increased the yields of bean, okra, and corn by 30%, 33%, and 19%, respectively”

✓ located verbatim in the source · abstract, characters 1478–1581 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Direct crop inoculation had no detectable effects on soil AMF community diversity or structure.

Taxon
bean, okra, and corne1
Treatment
direct crop inoculatione1
Interactor
AMFe1symbiont
Outcome
soil AMF community diversity or structuree1
Direction
no detectable effectse1

Not reportedComparator

Verbatim

e1

“direct crop inoculation increased the yields of bean, okra, and corn by 30%, 33%, and 19%, respectively, and intensified soil enzyme activity, with no detectable effects on soil AMF community diversity or structure”

✓ located verbatim in the source · abstract, characters 1478–1692 · show in text ↑

mycorrhiza · soil: biology · Zea mays (maize)Results differ

24 papers: 23 positive, 1 negative or no effect

22 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. positive

20 papers more in this group

See all 24 papers →

inoculation · soil: physics · Abelmoschus esculentus (okra)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

inoculation · soil: physics · Phaseolus vulgaris (common bean)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

cover crop · yield · Abelmoschus esculentus (okra)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

2 in the opposite direction

  1. positive
  2. positive
See all 3 papers →

inoculation · soil: physics · Zea mays (maize)Positive and negative results

11 papers: 8 positive, 3 negative or no effect

2 papers in the same direction as this paper · 8 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

cover crop · yield · Phaseolus vulgaris (common bean)Positive and negative results

19 papers: 8 positive, 11 negative or no effect

10 papers in the same direction as this paper · 8 in the opposite direction

  1. positive
  2. positive
  3. positive

15 papers more in this group

See all 19 papers →

inoculation · yield · Abelmoschus esculentus (okra)Positive and negative results

52 papers: 48 positive, 4 negative or no effect

47 papers in the same direction as this paper · 4 in the opposite direction

  1. no effect
  2. no effect
  3. negative

48 papers more in this group

See all 52 papers →

inoculation · soil: biology · Zea mays (maize)Positive and negative results

65 papers: 57 positive, 8 negative or no effect

56 papers in the same direction as this paper · 7 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. no effect
  3. no effect

61 papers more in this group

See all 65 papers →

8 of 15 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.