Paper · 2026 · International Journal of Agriculture Environment and Food Sciences

Dose dependent phytotoxic effects of Cd, Ni, and Cu on cauliflower in vitro

Ecem Kara

8
claims
23
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by

Abstract

This study was carried out to evaluate the effects of cadmium (Cd), nickel (Ni), and copper (Cu), which are significant components of industrial pollution, on germination and early seedling development in cauliflower (Brassica oleracea var. botrytis). Experiments were carried out in MS nutrient medium using heavy metal doses of 0 (control), 100, 300, and 500 µM under in vitro conditions. Morphological and physiological parameters such as germination percentage, germination index, mean germination time, shoot-root length, and fresh and dry weight were examined. The results showed that the phytotoxic effect varied depending on the metal type and dose. Cd particularly suppressed germination at high doses (42.00% at Cd-500). Cu showed toxic effects even at low doses, with germination values varying between 50.00% and 53.00% between treatments. Ni, on the other hand, showed a stimulating effect on some parameters, such as root length (50.73 mm at Ni-100), at low and medium doses. The longest shoot length was recorded in the control group (42.40 mm), while significant reductions were observed at higher metal doses. Correlation analysis revealed positive relationships between growth parameters and a negative relationship between root dry weight and germination measurements. PCA showed that most of the variance was concentrated in the first three components, which together explained 73.4% of the total variation.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cd at high doses suppressed cauliflower germination (42.00% at Cd-500).

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Treatment
Cde1
Outcome
germinatione1
Direction
suppressede1
Value
42.00%e1
Setting
in vitrot0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cauliflower in vitro”

✓ located verbatim in the source · title, characters 55–75 · show in text ↑
e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e1

“Cd particularly suppressed germination at high doses (42.00% at Cd-500)”

✓ located verbatim in the source · abstract, characters 658–729 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cu had toxic effects on cauliflower germination even at low doses, with germination of 50.00% to 53.00%.

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Treatment
Cue2
Outcome
germination valuese2
Direction
decreaseinferrede2
Value
50.00% and 53.00%e2
Setting
in vitrot0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cauliflower in vitro”

✓ located verbatim in the source · title, characters 55–75 · show in text ↑
e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e2

“Cu showed toxic effects even at low doses, with germination values varying between 50.00% and 53.00% between treatments”

✓ located verbatim in the source · abstract, characters 731–850 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ni had a stimulating effect on cauliflower root length at low and medium doses (50.73 mm at Ni-100).

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Treatment
Nie3
Outcome
root lengthe3
Direction
stimulating effecte3
Value
50.73 mme3
Qualifier
at low and medium dosese3
Setting
in vitrot0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cauliflower in vitro”

✓ located verbatim in the source · title, characters 55–75 · show in text ↑
e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e3

“Ni, on the other hand, showed a stimulating effect on some parameters, such as root length (50.73 mm at Ni-100), at low and medium doses”

✓ located verbatim in the source · abstract, characters 852–988 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The control group had the longest cauliflower shoots (42.40 mm).

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Outcome
shoot lengthe4
Value
42.40 mme4
Qualifier
control groupe4
Setting
in vitrot0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cauliflower in vitro”

✓ located verbatim in the source · title, characters 55–75 · show in text ↑
e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e4

“The longest shoot length was recorded in the control group (42.40 mm)”

✓ located verbatim in the source · abstract, characters 990–1059 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Higher metal doses significantly reduced cauliflower shoot length relative to the control group.

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Treatment
higher metal dosese5
Outcome
shoot lengthe4
Direction
reductionse5
Comparator
control groupe4
Setting
in vitrot0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cauliflower in vitro”

✓ located verbatim in the source · title, characters 55–75 · show in text ↑
e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e4

“The longest shoot length was recorded in the control group (42.40 mm)”

✓ located verbatim in the source · abstract, characters 990–1059 · show in text ↑
e5

“significant reductions were observed at higher metal doses”

✓ located verbatim in the source · abstract, characters 1067–1125 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Growth parameters of cauliflower were positively correlated with each other.

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Outcome
growth parameterse6
Direction
positivee6
Setting
in vitrot0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“cauliflower in vitro”

✓ located verbatim in the source · title, characters 55–75 · show in text ↑
e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e6

“Correlation analysis revealed positive relationships between growth parameters”

✓ located verbatim in the source · abstract, characters 1127–1205 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Root dry weight was negatively related to germination measurements in cauliflower.

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Organ / stage
roote7
Outcome
germination measurementse7
Direction
negativee7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e7

“a negative relationship between root dry weight and germination measurements”

✓ located verbatim in the source · abstract, characters 1210–1286 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The first three principal components explained 73.4% of the total variation in the cauliflower data.

Taxon
Brassica oleracea var. botrytisBrassica oleracea var. botrytise0
Outcome
total variatione1
Value
73.4%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“cauliflower (Brassica oleracea var. botrytis)”

✓ located verbatim in the source · abstract, characters 205–250 · show in text ↑
e1

“PCA showed that most of the variance was concentrated in the first three components, which together explained 73.4% of the total variation”

✓ located verbatim in the source · abstract, characters 1288–1426 · show in text ↑

nickel atom · growth · Brassica oleracea (wild cabbage)Results differ

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. negative
See all 3 papers →

cadmium atom · growth · Brassica oleracea (wild cabbage)Results differ

16 papers: 2 positive, 14 negative or no effect

13 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

12 papers more in this group

See all 16 papers →

copper atom · growth · Brassica oleracea (wild cabbage)Positive and negative results

8 papers: 2 positive, 6 negative or no effect

5 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. positive
  3. negative

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.