Abstract
Abstract BASIC PENTACYSTEINE (BPC) transcription factors are essential regulators of plant growth and development. However, BPC functions and the related molecular mechanisms during cucumber (Cucumis sativus L.) responses to abiotic stresses, especially salt stress, remain unknown. We previously determined that salt stress induces CsBPC expression in cucumber. In this study, Csbpc2 transgene-free cucumber plants were created using a CRISPR/Cas9-mediated editing system to explore CsBPC functions associated with the salt stress response. The Csbpc2 mutants had a hypersensitive phenotype, with increased leaf chlorosis, decreased biomass, and increased malondialdehyde and electrolytic leakage levels under salt stress conditions. Additionally, a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities, which led to the accumulation of hydrogen peroxide and superoxide radicals. Furthermore, the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities, resulting in decreased Na+ efflux and increased K+ efflux. These findings suggest that CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation, reactive oxygen species scavenging, and ion homeostasis-related regulatory pathways. However, CsBPC2 also affected ABA signaling. The mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related genes. Our results indicate that CsBPC2 may enhance the cucumber response to salt stress. It may also function as an important regulator of ABA biosynthesis and signal transduction. These findings will enrich our understanding of the biological functions of BPCs, especially their roles in abiotic stress responses, thereby providing the theoretical basis for improving crop salt tolerance.
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Claims 12
Claim · model-written summary
Csbpc2 mutants showed salt hypersensitivity and greater leaf chlorosis.
- Taxon
- cucumber
Cucumis sativust0 - Material
- Csbpc2 mutantse0
- Treatment
- Csbpc2 mutantse0
- Outcome
- leaf chlorosise0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Csbpc2 mutants had a hypersensitive phenotype, with increased leaf chlorosis”
t0“cucumber”
Claim · model-written summary
Csbpc2 mutants had lower biomass under salt stress.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Csbpc2 mutantse1
- Outcome
- biomasse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased biomass”
t0“cucumber”
e1“Csbpc2 mutants”
Claim · model-written summary
Csbpc2 mutants had higher malondialdehyde and electrolyte leakage under salt stress.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Csbpc2 mutantse1
- Outcome
- malondialdehyde and electrolytic leakage levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased malondialdehyde and electrolytic leakage levels under salt stress conditions.”
t0“cucumber”
e1“Csbpc2 mutants”
Claim · model-written summary
CsBPC2 mutation reduced proline and soluble sugar contents.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutated CsBPC2e0
- Gene
- CsBPC2e0
- Outcome
- proline and soluble sugar contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a mutated CsBPC2 resulted in decreased proline and soluble sugar contents”
t0“cucumber”
Claim · model-written summary
CsBPC2 mutation reduced antioxidant enzyme activity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutated CsBPC2e0
- Outcome
- antioxidant enzyme activitiese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities”
t0“cucumber”
Claim · model-written summary
CsBPC2 mutation led to accumulation of hydrogen peroxide and superoxide radicals.
- Taxon
- cucumberCucumis sativust0
- Outcome
- accumulation of hydrogen peroxide and superoxide radicalse0
- Qualifier
- which led toe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which led to the accumulation of hydrogen peroxide and superoxide radicals.”
t0“cucumber”
Claim · model-written summary
CsBPC2 mutation inhibited salt-induced PM-H+-ATPase and V-H+-ATPase activities.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutation to CsBPC2e0
- Outcome
- PM-H+-ATPase and V-H+-ATPase activitiese0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities”
t0“cucumber”
Claim · model-written summary
CsBPC2 mutation decreased Na+ efflux.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutation to CsBPC2e1
- Outcome
- Na+ effluxe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in decreased Na+ efflux”
t0“cucumber”
e1“the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase”
Claim · model-written summary
CsBPC2 mutation increased K+ efflux.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutation to CsBPC2e1
- Outcome
- K+ effluxe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased K+ efflux”
t0“cucumber”
e1“the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase”
Claim · model-written summary
CsBPC2 mutation adversely affected salt-induced ABA biosynthesis.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutation to CsBPC2e0
- Outcome
- salt-induced ABA biosynthesise0
- Direction
- adversely affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis”
t0“cucumber”
Claim · model-written summary
CsBPC2 mutation adversely affected ABA signaling gene expression.
- Taxon
- cucumberCucumis sativust0
- Treatment
- mutation to CsBPC2e0
- Outcome
- expression of ABA signaling-related genese0
- Direction
- adversely affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related genes.”
t0“cucumber”
Claim · model-written summary
Authors infer CsBPC2 may enhance cucumber salt response through osmoregulation, ROS scavenging and ion homeostasis.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsBPC2e0
- Outcome
- salt stress resistancee0
- Qualifier
- through its effects on osmoregulation, reactive oxygen species scavenging, and ion homeostasis-related regulatory pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation, reactive oxygen species scavenging, and ion homeostasis-related regulatory pathways.”
t0“cucumber”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.