Abstract
Introduction: Previous studies have shown that m6A methylation has a certain regulatory role in the replication of human viruses and plant viruses. Particularly, the m6A methylation in the host endogenous gene significantly decreased after the infection of potato virus Y (PVY). Methods: Here, we cloned and characterized N6-adenosine-methyltransferase MTA70-like (ToMTA70) from tomato. The ToMTA70 was constructed into the pMDC32 binary vector. To create the ToMTA70 mutant, sequence of the MTA70 superfamily domain (493-653) with PDS and GUS sequences, respectively. The effect of the ToMTA70 on viral accumulation was studied using an Agrobacterium-mediated transient expression assay. Results: A conserved MT-A70 superfamily domain in ToMTA70 was identified by encoding sequence and structural analyses, which indicating ToMTA70 might involve in m6A methylation. The transient expression of ToMTA70 inhibited viral accumulation of PVY. When the conserved domain of MTA70 super family was replaced by PDS and GUS (ToMTA70-PDS or ToMTA70-GUS), the PVY infection was increased in plants comparing with the control. However, transiently expression of ToMTA70 did not show any influence on TVMV and TMV infection. Discussion: This suggests that ToMTA70 participate in plant antiviral responses of PVY, which can be applied for engineering the anti-viral strategy.
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Claims 7
Claim · model-written summary
ToMTA70 contains a conserved MT-A70 superfamily domain.
- Taxon
- tomato
Solanum lycopersicume1 - Gene
- ToMTA70e0
- Outcome
- conserved MT-A70 superfamily domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A conserved MT-A70 superfamily domain in ToMTA70 was identified by encoding sequence and structural analyses”
e1“N6-adenosine-methyltransferase MTA70-like (ToMTA70) from tomato”
Claim · model-written summary
Transient ToMTA70 expression inhibited PVY accumulation.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- transient expression of ToMTA70e0
- Interactor
- PVY
Potato virus Ye0pathogen - Gene
- ToMTA70e0
- Outcome
- viral accumulation of PVYe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transient expression of ToMTA70 inhibited viral accumulation of PVY.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Replacing the MTA70 domain with PDS increased PVY infection relative to control.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- replaced by PDSe0
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- ToMTA70-PDSe0
- Outcome
- PVY infectione0
- Direction
- increasede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the conserved domain of MTA70 super family was replaced by PDS and GUS (ToMTA70-PDS or ToMTA70-GUS), the PVY infection was increased in plants comparing with the control.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Replacing the MTA70 domain with GUS increased PVY infection relative to control.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- replaced by PDS and GUSe0
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- ToMTA70-GUSe0
- Outcome
- PVY infectione0
- Direction
- increasede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When the conserved domain of MTA70 super family was replaced by PDS and GUS (ToMTA70-PDS or ToMTA70-GUS), the PVY infection was increased in plants comparing with the control.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient ToMTA70 expression showed no influence on TVMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- transiently expression of ToMTA70e0
- Interactor
- TVMV
Tobacco vein mottling viruse0pathogen - Gene
- ToMTA70e0
- Outcome
- TVMVe0
- Direction
- did not show any influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transiently expression of ToMTA70 did not show any influence on TVMV and TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient ToMTA70 expression showed no influence on TMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- transiently expression of ToMTA70e0
- Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- ToMTA70e0
- Outcome
- TMV infectione0
- Direction
- did not show any influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transiently expression of ToMTA70 did not show any influence on TVMV and TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors suggest ToMTA70 may be used for an antiviral strategy against PVY.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- ToMTA70e0
- Outcome
- engineering the anti-viral strategye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ToMTA70 participate in plant antiviral responses of PVY, which can be applied for engineering the anti-viral strategy.”
t0“Nicotiana benthamiana”
The same questions in other papers
transgenic · Tobacco vein mottling virus · Nicotiana benthamianaPositive and negative results
1 in the opposite direction
- positive
transgenic · Tobacco mosaic virus · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
transgenic · Potato virus Y · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.