Abstract
Aged pea Pisum sativum L. var Alaska epicotyl tissue was wounded by excising the apical 10 or 20 millimeters and incubating the excised segments upright in buffer. Wounding induced a very rapid formation of polysomes which was accompanied by minor increases in ribosomes, mRNA, and poly(A) and by a doubling of the in vivo protein synthesizing capacity. This increase in protein synthesis in vivo was matched by a similar increase in polypeptide synthesis in vitro in wheat germ reactions primed by polysomes. However, in vitro reactions primed by total and polysomal RNA from wounded tissue were affected much less.Two-dimensional gel patterns of silver-stained proteins accumulated in vivo were almost unchanged, even after 6 hours of wounding, since only two spots decreased in intensity and none increased. In contrast, two-dimensional gel fluorographs of polypeptides generated in vitro by both total RNA and polysomal RNA showed numerous changes within 3 hours of wounding. Of the more than 200 spots visualized by fluorography, 17 decreased and 26 increased when total RNA from wounded tissue was used; 15 decreased and 10 increased when polysomal RNA was used. Those polypeptides that decreased after wounding were generally of lower molecular weight; those which increased were of higher molecular weight.Although wounding must be affecting transcription insofar as different mRNAs must be present to encode different polypeptides, its major effect appears to be on translation, presumably through formation of ribosomes with greater translational efficiency.
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Claims 7
Claim · model-written summary
Wounding rapidly formed polysomes, slightly increased RNA and ribosomes, and doubled protein synthesis capacity.
- Taxon
- Pisum sativum L.
Lathyrus oleraceuse1 - Material
- Alaskae1
- Organ / stage
- epicotyl tissuee1
- Treatment
- Woundinge0
- Outcome
- in vivo protein synthesizing capacitye0
- Direction
- doublinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding induced a very rapid formation of polysomes which was accompanied by minor increases in ribosomes, mRNA, and poly(A) and by a doubling of the in vivo protein synthesizing capacity.”
e1“pea Pisum sativum L. var Alaska epicotyl tissue”
Claim · model-written summary
Polysomes from wounded tissue raised in-vitro polypeptide synthesis similarly to in-vivo synthesis, while RNA-primed reactions changed less.
Not reportedTaxon
Verbatim
e0“This increase in protein synthesis in vivo was matched by a similar increase in polypeptide synthesis in vitro in wheat germ reactions primed by polysomes. However, in vitro reactions primed by total and polysomal RNA from wounded tissue were affected much less.”
Claim · model-written summary
Accumulated in-vivo proteins changed little after 6 hours of wounding; two spots decreased and none increased.
- Outcome
- silver-stained proteins accumulated in vivoe0
- Value
- two spotse0
- Qualifier
- after 6 hours of woundinge0
Not reportedTaxon
Verbatim
e0“Two-dimensional gel patterns of silver-stained proteins accumulated in vivo were almost unchanged, even after 6 hours of wounding, since only two spots decreased in intensity and none increased.”
Claim · model-written summary
Total RNA from wounded tissue yielded 17 decreased and 26 increased in-vitro polypeptide spots.
Not reportedTaxon
Verbatim
e0“Of the more than 200 spots visualized by fluorography, 17 decreased and 26 increased when total RNA from wounded tissue was used”
Claim · model-written summary
Polysomal RNA from wounded tissue yielded 15 decreased and 10 increased in-vitro polypeptide spots.
Not reportedTaxon
Verbatim
e0“15 decreased and 10 increased when polysomal RNA was used”
Claim · model-written summary
Polypeptides decreasing after wounding tended to be lower molecular weight and those increasing higher molecular weight.
Not reportedTaxon
Verbatim
e0“Those polypeptides that decreased after wounding were generally of lower molecular weight; those which increased were of higher molecular weight.”
Claim · model-written summary
The authors interpret translation as the major target of wounding.
Not reportedTaxon
Verbatim
e0“its major effect appears to be on translation, presumably through formation of ribosomes with greater translational efficiency.”
The same questions in other papers
mechanical · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.