Abstract
ABSTRACT Anthracnose is a devastating fungal disease in tea plants caused by the pathogen Colletotrichum species. It severely affects tea yield and quality. Pathogenesis‐related (PR) genes play indispensable roles in plant resistance to fungal pathogens. Our transcriptome data showed that PR10 family genes are involved in the immune response to anthracnose infection in tea plants; however, the underlying regulatory mechanisms remain unclear. Here, we identified a PR10 family gene, CsPR10‐9 , which was highly induced by Colletotrichum gloeosporioides infection and salicylic acid (SA)/methyl jasmonate (MeJA) treatments. Transient silencing of CsPR10‐9 substantially compromised the disease resistance of tea plants, and this was accompanied by reduced activities of the antioxidant enzymes, peroxidase (POD) and superoxide dismutase (SOD), resulting in elevated reactive oxygen species (ROS) levels, decreased SA content, and increased jasmonic acid (JA) content. Transient overexpression of CsPR10‐9 in tobacco leaves showed that the lesion area on CsPR10‐9‐overexpressing leaves was significantly smaller than the control leaves, accompanied by decreased H 2 O 2 level and increased POD and SOD activities. CsPR10‐9 was negatively regulated by an R2R3‐type MYB transcription factor, CsMYB72, which directly bound to MYB transcription factor binding site cis ‐acting elements in its promoter to inhibit the expression of CsPR10‐9 . CsMYB72 is localised in the nucleus and participate in the response to anthracnose infection and SA/MeJA treatment. Moreover, silencing of CsMYB72 significantly enhanced disease resistance, increased POD and SOD activities, reduced ROS levels, increased SA content, and decreased JA content. Taken together, our results revealed that the CsMYB72‐CsPR10‐9 module regulates the SA/JA‐mediated defence response of tea plants to anthracnose. This study provides new molecular targets and a theoretical foundation for breeding of disease‐resistant tea germplasms.
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Claims 17
Claim · model-written summary
Tea PR10 family genes were implicated in the immune response to anthracnose.
Verbatim
e0“PR10 family genes are involved in the immune response to anthracnose infection in tea plants”
t0“Tea Plants”
Claim · model-written summary
CsPR10-9 was strongly induced by C. gloeosporioides infection and salicylic acid and methyl jasmonate treatments.
- Interactor
- Colletotrichum gloeosporioides
Colletotrichum gloeosporioidese0t0pathogen
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsPR10‐9 , which was highly induced by Colletotrichum gloeosporioides infection and salicylic acid (SA)/methyl jasmonate (MeJA) treatments”
t0“Tea Plants”
Claim · model-written summary
Transient CsPR10-9 silencing compromised tea disease resistance.
Not reportedComparator
Verbatim
e0“Transient silencing of CsPR10‐9 substantially compromised the disease resistance of tea plants”
t0“Tea Plants”
Claim · model-written summary
CsPR10-9 silencing reduced peroxidase and superoxide dismutase activities.
Not reportedComparator
Verbatim
e0“this was accompanied by reduced activities of the antioxidant enzymes, peroxidase (POD) and superoxide dismutase (SOD)”
t0“Tea Plants”
e1“Transient silencing of CsPR10‐9”
Claim · model-written summary
CsPR10-9 silencing elevated reactive oxygen species levels.
Not reportedComparator
Verbatim
e0“resulting in elevated reactive oxygen species (ROS) levels”
t0“Tea Plants”
e1“Transient silencing of CsPR10‐9”
Claim · model-written summary
CsPR10-9 silencing decreased salicylic acid content.
Not reportedComparator
Verbatim
e0“decreased SA content”
t0“Tea Plants”
e1“Transient silencing of CsPR10‐9”
Claim · model-written summary
CsPR10-9 silencing increased jasmonic acid content.
Not reportedComparator
Verbatim
e0“increased jasmonic acid (JA) content”
t0“Tea Plants”
e1“Transient silencing of CsPR10‐9”
Claim · model-written summary
CsPR10-9 overexpression in tobacco reduced lesion area relative to control leaves.
- Taxon
- tobacco
Nicotiana tabacume0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of CsPR10‐9 in tobacco leaves showed that the lesion area on CsPR10‐9‐overexpressing leaves was significantly smaller than the control leaves”
t0“Tea Plants”
Claim · model-written summary
CsPR10-9 overexpression in tobacco decreased hydrogen peroxide and increased POD and SOD activities.
- Taxon
- tobaccoNicotiana tabacume0t0e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accompanied by decreased H 2 O 2 level and increased POD and SOD activities”
t0“Tea Plants”
e1“Transient overexpression of CsPR10‐9 in tobacco leaves”
Claim · model-written summary
CsMYB72 directly bound cis elements in the CsPR10-9 promoter and inhibited CsPR10-9 expression.
Verbatim
e0“CsMYB72, which directly bound to MYB transcription factor binding site cis ‐acting elements in its promoter to inhibit the expression of CsPR10‐9”
t0“Tea Plants”
Claim · model-written summary
CsMYB72 localizes to the nucleus and responds to anthracnose infection and SA/MeJA treatment.
Verbatim
e0“CsMYB72 is localised in the nucleus and participate in the response to anthracnose infection and SA/MeJA treatment”
t0“Tea Plants”
Claim · model-written summary
CsMYB72 silencing significantly enhanced tea disease resistance.
Not reportedComparator
Verbatim
e0“silencing of CsMYB72 significantly enhanced disease resistance”
t0“Tea Plants”
Claim · model-written summary
CsMYB72 silencing increased POD and SOD activities.
Not reportedComparator
Verbatim
e0“silencing of CsMYB72 significantly enhanced disease resistance, increased POD and SOD activities”
t0“Tea Plants”
e1“silencing of CsMYB72”
Claim · model-written summary
CsMYB72 silencing reduced ROS levels.
Not reportedComparator
Verbatim
e0“reduced ROS levels”
t0“Tea Plants”
e1“silencing of CsMYB72”
Claim · model-written summary
CsMYB72 silencing increased SA content.
Not reportedComparator
Verbatim
e0“increased SA content”
t0“Tea Plants”
e1“silencing of CsMYB72”
Claim · model-written summary
CsMYB72 silencing decreased JA content.
Not reportedComparator
Verbatim
e0“decreased JA content”
t0“Tea Plants”
e1“silencing of CsMYB72”
Claim · model-written summary
The CsMYB72–CsPR10-9 module regulates the SA/JA-mediated defense response to tea anthracnose.
Verbatim
e0“CsMYB72‐CsPR10‐9 module regulates the SA/JA‐mediated defence response of tea plants to anthracnose”
t0“Tea Plants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.