Abstract
This study integrated transcriptome and hormone profiling of Colletotrichum camelliae -infected tea leaves to identify the methyl esterase ( MES ) gene family. Genome-wide analysis identified 17 MES genes, with CsMES4 as a crucial candidate localized in the nucleus and cell membrane. Through prokaryotic expression, purification, in vitro enzymatic assays, and transient silencing experiments in tea plants, we demonstrated that CsMES4 hydrolyze methyl salicylate (MeSA), methyl indole-3-acetate (MeIAA), and methyl jasmonate (MeJA) to produce salicylic acid, indole-3-acetic acid, and jasmonic acid, respectively. Transient silencing of CsMES4 reduced these hormones and enlarged lesions, confirming its role in disease resistance. Key catalytic residues predicted by docking were validated by mutagenesis: the F60L mutation significantly decreased CsMES4’s catalytic activity toward all three substrates, while the S127Y mutation enhanced its catalytic efficiency. This study highlights the pivotal role of CsMES4 in phytohormone metabolism and provides insights into its multifunctional catalytic mechanism in tea plants.
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Claims 9
Claim · model-written summary
Genome-wide analysis identified 17 MES genes.
Verbatim
e0“Genome-wide analysis identified 17 MES genes”
t0“Tea Plants”
Claim · model-written summary
CsMES4 was localized to the nucleus and cell membrane.
Verbatim
e0“CsMES4 as a crucial candidate localized in the nucleus and cell membrane.”
t0“Tea Plants”
Claim · model-written summary
CsMES4 hydrolyzed methyl salicylate to salicylic acid.
Verbatim
e0“CsMES4 hydrolyze methyl salicylate (MeSA), methyl indole-3-acetate (MeIAA), and methyl jasmonate (MeJA) to produce salicylic acid, indole-3-acetic acid, and jasmonic acid, respectively.”
t0“Tea Plants”
Claim · model-written summary
CsMES4 hydrolyzed methyl indole-3-acetate to indole-3-acetic acid.
Verbatim
e0“CsMES4 hydrolyze methyl salicylate (MeSA), methyl indole-3-acetate (MeIAA), and methyl jasmonate (MeJA) to produce salicylic acid, indole-3-acetic acid, and jasmonic acid, respectively.”
t0“Tea Plants”
Claim · model-written summary
CsMES4 hydrolyzed methyl jasmonate to jasmonic acid.
Verbatim
e0“CsMES4 hydrolyze methyl salicylate (MeSA), methyl indole-3-acetate (MeIAA), and methyl jasmonate (MeJA) to produce salicylic acid, indole-3-acetic acid, and jasmonic acid, respectively.”
t0“Tea Plants”
Claim · model-written summary
Transient CsMES4 silencing reduced the three hormones.
- Taxon
- Tea Plantst0
- Treatment
- Transient silencing of CsMES4e0
- Gene
- CsMES4e0
- Outcome
- these hormonese0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Transient silencing of CsMES4 reduced these hormones and enlarged lesions”
t0“Tea Plants”
Claim · model-written summary
Transient CsMES4 silencing enlarged lesions.
- Taxon
- Tea Plantst0
- Treatment
- Transient silencing of CsMES4e0
- Gene
- CsMES4e0
- Outcome
- lesionse0
- Direction
- enlargede0
Not reportedComparator
Verbatim
e0“Transient silencing of CsMES4 reduced these hormones and enlarged lesions”
t0“Tea Plants”
Claim · model-written summary
F60L significantly decreased CsMES4 catalytic activity toward all three substrates.
- Taxon
- Tea Plantst0
- Treatment
- F60L mutatione0
- Gene
- CsMES4e0
- Outcome
- CsMES4’s catalytic activitye0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“the F60L mutation significantly decreased CsMES4’s catalytic activity toward all three substrates”
t0“Tea Plants”
Claim · model-written summary
S127Y enhanced CsMES4 catalytic efficiency.
Not reportedComparator
Verbatim
e0“the S127Y mutation enhanced its catalytic efficiency”
t0“Tea Plants”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.