Abstract
Abstract Gray blight is a serious foliar disease that significantly threatens tea plant cultivation. Although dynamic histone methylation was reported in regulating plant immunity, the specific roles of this epigenetic modification in tea plant disease resistance have yet to be fully elucidated. This study demonstrates that the protein arginine methyltransferase CsPRMT5, which catalyzes the symmetric dimethylation of histone H4R3 (H4R3sme2), is involved in the tea plant response to gray blight. Transcription of CsPRMT5 and the level of histone H4R3 methylation in tea were downregulated following infection by the fungal pathogen Pseudopestalotiopsis (Ps). A negative correlation was observed between the resistance of tea plants to Ps and the expression level of CsPRMT5 across various cultivars. Downregulation of CsPRMT5 expression led to reduced H4R3sme2 levels, elevated expression of defense-related genes, and lower reactive oxygen species (ROS) production after Ps infection, thus enhancing pathogen resistance of tea. Furthermore, complementation of Atprmt5 mutant with CsPRMT5 restored the susceptibility to Ps infection in Arabidopsis. Chromatin Immunoprecipitation Sequencing (ChIP-seq)and Chromatin Immunoprecipitation quantitative PCR (ChIP-qPCR) analyses revealed that CsPRMT5 binds to defense-related genes, including CsMAPK3, and regulates their expression through H4R3sme2 modification. Collectively, the results indicate that CsPRMT5 negatively regulates the immune response to pathogens through repressing CsMAPK3 expression in tea plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Ps infection downregulated CsPRMT5 transcription in tea.
- Taxon
- teae0
- Treatment
- infection by the fungal pathogen Pseudopestalotiopsis (Ps)e0
- Interactor
- PseudopestalotiopsisPseudopestalotiopsise0pathogen
- Gene
- CsPRMT5e0
- Outcome
- Transcription of CsPRMT5e0
- Direction
- downregulatede0
Not reportedComparator
Verbatim
e0“Transcription of CsPRMT5 and the level of histone H4R3 methylation in tea were downregulated following infection by the fungal pathogen Pseudopestalotiopsis (Ps).”
Claim · model-written summary
Ps infection downregulated histone H4R3 methylation in tea.
- Taxon
- teae0
- Treatment
- infection by the fungal pathogen Pseudopestalotiopsis (Ps)e0
- Interactor
- PseudopestalotiopsisPseudopestalotiopsise0pathogen
- Gene
- histone H4R3e0
- Outcome
- level of histone H4R3 methylatione0
- Direction
- downregulatede0
Not reportedComparator
Verbatim
e0“Transcription of CsPRMT5 and the level of histone H4R3 methylation in tea were downregulated following infection by the fungal pathogen Pseudopestalotiopsis (Ps).”
Claim · model-written summary
CsPRMT5 expression was negatively correlated with tea resistance to Ps across cultivars.
Verbatim
e0“A negative correlation was observed between the resistance of tea plants to Ps and the expression level of CsPRMT5 across various cultivars.”
Claim · model-written summary
CsPRMT5 downregulation reduced H4R3sme2 levels.
- Taxon
- teat0
- Treatment
- Downregulation of CsPRMT5 expressione0
- Gene
- CsPRMT5e0
- Outcome
- H4R3sme2 levelse0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Downregulation of CsPRMT5 expression led to reduced H4R3sme2 levels”
t0“tea plants”
Claim · model-written summary
CsPRMT5 downregulation elevated defense-related gene expression.
- Taxon
- tea plantst0
- Treatment
- Downregulation of CsPRMT5 expressione0
- Gene
- CsPRMT5e0
- Outcome
- expression of defense-related genese0
- Direction
- elevatede0
Not reportedComparator
Verbatim
e0“Downregulation of CsPRMT5 expression led to reduced H4R3sme2 levels, elevated expression of defense-related genes”
t0“tea plants”
Claim · model-written summary
CsPRMT5 downregulation lowered ROS production after Ps infection.
- Taxon
- tea plantst0
- Treatment
- Downregulation of CsPRMT5 expressione0
- Interactor
- Pse0pathogen
- Gene
- CsPRMT5e0
- Outcome
- reactive oxygen species (ROS) productione0
- Direction
- lowere0
Not reportedComparator
Verbatim
e0“Downregulation of CsPRMT5 expression led to reduced H4R3sme2 levels, elevated expression of defense-related genes, and lower reactive oxygen species (ROS) production after Ps infection”
t0“tea plants”
Claim · model-written summary
CsPRMT5 downregulation enhanced tea pathogen resistance.
- Taxon
- teae0
- Treatment
- Downregulation of CsPRMT5 expressione0
- Interactor
- Pse0pathogen
- Gene
- CsPRMT5e0
- Outcome
- pathogen resistance of teae0
- Direction
- enhancinge0
Not reportedComparator
Verbatim
e0“Downregulation of CsPRMT5 expression led to reduced H4R3sme2 levels, elevated expression of defense-related genes, and lower reactive oxygen species (ROS) production after Ps infection, thus enhancing pathogen resistance of tea.”
Claim · model-written summary
CsPRMT5 complementation restored Ps susceptibility in Arabidopsis Atprmt5 mutant.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- Atprmt5 mutante0
- Treatment
- complementation of Atprmt5 mutant with CsPRMT5e0
- Interactor
- Pse0pathogen
- Gene
- CsPRMT5e0
- Outcome
- susceptibility to Ps infectione0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“complementation of Atprmt5 mutant with CsPRMT5 restored the susceptibility to Ps infection in Arabidopsis.”
Claim · model-written summary
CsPRMT5 bound defense-related genes including CsMAPK3.
Verbatim
e0“CsPRMT5 binds to defense-related genes, including CsMAPK3”
t0“tea plants”
Claim · model-written summary
CsPRMT5 regulated defense-related gene expression through H4R3sme2 modification.
Verbatim
e0“CsPRMT5 binds to defense-related genes, including CsMAPK3, and regulates their expression through H4R3sme2 modification.”
t0“tea plants”
Claim · model-written summary
CsPRMT5 negatively regulated immunity by repressing CsMAPK3 expression in tea.
Verbatim
e0“CsPRMT5 negatively regulates the immune response to pathogens through repressing CsMAPK3 expression in tea plants.”
The same questions in other papers
infection · molecular: expression · Camellia sinensis (black tea)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
3 papers more in this group
See all 7 papers →loss of function · molecular: expression · Camellia sinensis (black tea)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →loss of function · disease · Camellia sinensis (black tea)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →loss of function · physiology: oxidative · Camellia sinensis (black tea)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.