Abstract
Anthocyanins are key metabolites that determine red pigmentation in pear skin (Pyrus spp.) and their biosynthesis is controlled by multiple transcription factors. Although phytochrome-interacting factors (PIFs) of the bHLH family have been shown to regulate anthocyanin biosynthesis in Arabidopsis thaliana, their genome-wide identification and regulatory mechanisms in pear (Pyrus spp.) anthocyanin synthesis remain unclear. Here, we characterized PIFs family in pear, identifying eight PbPIF proteins. Promoter cis-elements and expression patterns analysis suggested that PbPIF3a and PbPIF4 might be involved in anthocyanin biosynthesis. Subcellular localization confirmed nuclear enrichment of PbPIF3a and PbPIF4. Functional studies demonstrated that overexpression of PbPIF3a and PbPIF4 significantly suppressed anthocyanin accumulation in fruit skins, downregulating key biosynthetic genes such as PbDFR and PbUFGT. In contrast, the silencing of related genes led to an enhancement of anthocyanin accumulation. Dual-luciferase reporter assays and yeast one-hybrid assays confirmed that PbPIF3a directly bound to the promoters of PbDFR and PbUFGT and repressed their transcriptional activation, while PbPIF4 specifically inhibited the activity of the PbDFR promoter. Taken together, we demonstrated that PbPIF3a and PbPIF4 negatively regulated pear fruit coloration by directly repressing the transcriptional activity of key anthocyanin biosynthesis genes, providing novel insights into PIF-mediated regulation of anthocyanin biosynthesis.
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Claims 11
Claim · model-written summary
Eight PbPIF proteins were identified in pear.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise0 - Gene
- PbPIF proteinse0
- Outcome
- PbPIF proteinse0
- Value
- eight PbPIF proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“characterized PIFs family in pear, identifying eight PbPIF proteins”
Claim · model-written summary
Promoter cis-elements and expression patterns suggested PbPIF3a and PbPIF4 involvement in anthocyanin biosynthesis.
- Taxon
- PearPyrus communist0
- Gene
- PbPIF3a and PbPIF4e0
- Outcome
- anthocyanin biosynthesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter cis-elements and expression patterns analysis suggested that PbPIF3a and PbPIF4 might be involved in anthocyanin biosynthesis.”
t0“Pear”
Claim · model-written summary
PbPIF3a was enriched in the nucleus.
- Taxon
- PearPyrus communist0
- Gene
- PbPIF3ae0
- Outcome
- nuclear enrichmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subcellular localization confirmed nuclear enrichment of PbPIF3a and PbPIF4.”
t0“Pear”
Claim · model-written summary
PbPIF4 was enriched in the nucleus.
- Taxon
- PearPyrus communist0
- Gene
- PbPIF4e0
- Outcome
- nuclear enrichmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subcellular localization confirmed nuclear enrichment of PbPIF3a and PbPIF4.”
t0“Pear”
Claim · model-written summary
PbPIF3a overexpression significantly suppressed anthocyanin accumulation in pear fruit skins.
- Taxon
- PearPyrus communist0
- Organ / stage
- fruit skinse0
- Treatment
- overexpression of PbPIF3ae0
- Gene
- PbPIF3ae0
- Outcome
- anthocyanin accumulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PbPIF3a and PbPIF4 significantly suppressed anthocyanin accumulation in fruit skins”
t0“Pear”
Claim · model-written summary
PbPIF4 overexpression significantly suppressed anthocyanin accumulation in pear fruit skins.
- Taxon
- PearPyrus communist0
- Organ / stage
- fruit skinse0
- Treatment
- overexpression of PbPIF3a and PbPIF4e0
- Gene
- PbPIF4e0
- Outcome
- anthocyanin accumulatione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PbPIF3a and PbPIF4 significantly suppressed anthocyanin accumulation in fruit skins”
t0“Pear”
Claim · model-written summary
PbPIF3a and PbPIF4 overexpression downregulated PbDFR and PbUFGT.
- Taxon
- PearPyrus communist0
- Treatment
- overexpression of PbPIF3a and PbPIF4e0
- Gene
- PbDFR and PbUFGTe0
- Outcome
- key biosynthetic genes such as PbDFR and PbUFGTe0
- Direction
- downregulatinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PbPIF3a and PbPIF4 significantly suppressed anthocyanin accumulation in fruit skins, downregulating key biosynthetic genes such as PbDFR and PbUFGT”
t0“Pear”
Claim · model-written summary
Silencing related genes enhanced anthocyanin accumulation.
- Taxon
- PearPyrus communist0
- Treatment
- silencing of related genese0
- Outcome
- anthocyanin accumulatione0
- Direction
- enhancemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the silencing of related genes led to an enhancement of anthocyanin accumulation”
t0“Pear”
Claim · model-written summary
PbPIF3a directly bound the PbDFR promoter and repressed transcriptional activation.
- Taxon
- PearPyrus communist0
- Gene
- PbPIF3ae0
- Outcome
- transcriptional activatione0
- Direction
- repressede0
- Qualifier
- promoters of PbDFRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PbPIF3a directly bound to the promoters of PbDFR and PbUFGT and repressed their transcriptional activation”
t0“Pear”
Claim · model-written summary
PbPIF3a directly bound the PbUFGT promoter and repressed transcriptional activation.
- Taxon
- PearPyrus communist0
- Gene
- PbPIF3ae0
- Outcome
- transcriptional activatione0
- Direction
- repressede0
- Qualifier
- promoters of PbDFR and PbUFGTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PbPIF3a directly bound to the promoters of PbDFR and PbUFGT and repressed their transcriptional activation”
t0“Pear”
Claim · model-written summary
PbPIF4 inhibited PbDFR promoter activity.
- Taxon
- PearPyrus communist0
- Gene
- PbPIF4e0
- Outcome
- activity of the PbDFR promotere0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PbPIF4 specifically inhibited the activity of the PbDFR promoter”
t0“Pear”
The same questions in other papers
loss of function · quality: phytochemicals · Pyrus communis (pear)Positive and negative results
1 paper in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.