Paper · 2025 · International Journal of Advanced Biochemistry Research

Study on impact of neem (Azadiracha indica) leaf extract on growth of field crops

Krishnaveni A; Kaviya P; Vijayalakshmi V; et al.

12
claims
12
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Experiments were conducted to investigate the allelopathic effects of neem (Azardirachta indica) leaf extract on selected field crops' seed germination and seedling vigor. Five crops viz., rice, maize, sorghum, finger millet, and black gram seeds were used in the study. Seeds of each crop were placed on filter paper saturated with six different concentrations of neem leaf extract (1%, 2%, 3%, 4%, 5%, and 6%), while water was used as the control. Qualitative and quantitative analysis of phytochemicals, including alkaloids, cardiac glycosides, terpenoids, steroids, saponins, phenolic compounds, flavonoids, carbohydrates, and proteins, was conducted using ethanol, chloroform, and diethyl ether extracts. The analysis revealed that neem leaves are richest in alkaloids (5.14%), followed by flavonoids (4.12%). The results showed that Azardirachta indica leaf extracts significantly reduced seed germination and seedling vigor in all crops. As the concentration of the neem leaf extract increased, germination percentages decreased across all crops, with reductions of 18% to 20% compared to the control. Blackgram exhibited the greatest reduction in germination, with germination rates dropping from 89% (water control) to 30% at the highest concentration of neem extract (6%). Regardless of crop type, seedling vigor also declined with increasing neem extract concentrations. Seedling vigor in the water control was recorded as 287.30, 723.1, 696.6, 348.6, and 232.16 for rice, sorghum, maize, black gram, and finger millet, respectively, while values in the 6% neem extract treatment were 161.2, 198.21, 226.4, 58.9, and 62.5, respectively. These results suggest that neem (Azardirachta indica) leaf extract exhibits allelopathic effects, inhibiting both seed germination and seedling growth across various crops.

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Claims 12

Observationobservedoriginal resultmeasured

Claim · model-written summary

Neem leaves contained 5.14% alkaloids.

Organ / stage
leavese0
Outcome
alkaloidse0
Value
5.14%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azadirachta indica
Azadirachta indica · TaxonVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The analysis revealed that neem leaves are richest in alkaloids (5.14%), followed by flavonoids (4.12%).”

✓ located verbatim in the source · abstract, characters 710–814 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Neem leaves contained 4.12% flavonoids.

Organ / stage
leavese0
Outcome
flavonoidse0
Value
4.12%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azadirachta indica
Azadirachta indica · TaxonVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The analysis revealed that neem leaves are richest in alkaloids (5.14%), followed by flavonoids (4.12%).”

✓ located verbatim in the source · abstract, characters 710–814 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Neem leaf extract significantly reduced seed germination across the tested crops.

Taxon
all cropse0
Treatment
Azardirachta indica leaf extractse0
Interactor
Azardirachta indicae0other
Outcome
seed germinatione0
Direction
reducede0

Not reportedComparator

Verbatim

e0

“The results showed that Azardirachta indica leaf extracts significantly reduced seed germination and seedling vigor in all crops.”

✓ located verbatim in the source · abstract, characters 815–944 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Neem leaf extract significantly reduced seedling vigor across the tested crops.

Taxon
all cropse0
Treatment
Azardirachta indica leaf extractse0
Interactor
Azardirachta indicae0other
Outcome
seedling vigore0
Direction
reducede0

Not reportedComparator

Verbatim

e0

“The results showed that Azardirachta indica leaf extracts significantly reduced seed germination and seedling vigor in all crops.”

✓ located verbatim in the source · abstract, characters 815–944 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing neem extract concentration decreased germination across crops by 18% to 20%.

Taxon
all cropse0
Treatment
neem leaf extracte0
Interactor
neemAzadirachta indicae0other
Outcome
germination percentagese0
Direction
decreasede0
Value
18% to 20%e0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“As the concentration of the neem leaf extract increased, germination percentages decreased across all crops, with reductions of 18% to 20% compared to the control.”

✓ located verbatim in the source · abstract, characters 945–1108 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Blackgram germination fell from 89% with water to 30% at 6% neem extract.

Taxon
BlackgramVigna mungoe0
Treatment
neem extract (6%)e0
Interactor
neemAzadirachta indicae0other
Outcome
germination ratese0
Direction
droppinge0
Value
30%e0
Qualifier
89%e0
Comparator
water controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Blackgram exhibited the greatest reduction in germination, with germination rates dropping from 89% (water control) to 30% at the highest concentration of neem extract (6%).”

✓ located verbatim in the source · abstract, characters 1109–1282 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Seedling vigor declined as neem extract concentration rose across crops.

Taxon
crope0
Treatment
neem extract concentrationse0
Interactor
neemAzadirachta indicae0other
Outcome
seedling vigore0
Direction
declinede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Regardless of crop type, seedling vigor also declined with increasing neem extract concentrations.”

✓ located verbatim in the source · abstract, characters 1283–1381 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

rice seedling vigor was 161.2 under 6% neem extract versus 287.30 with water.

Treatment
6% neem extract treatmente0
Interactor
neemAzadirachta indicae0other
Outcome
Seedling vigore0
Direction
decreaseinferrede0
Value
161.2e0
Qualifier
287.30e0
Comparator
water controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗
Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling vigor in the water control was recorded as 287.30, 723.1, 696.6, 348.6, and 232.16 for rice, sorghum, maize, black gram, and finger millet, respectively, while values in the 6% neem extract treatment were 161.2, 198.21, 226.4, 58.9, and 62.5, respectively.”

✓ located verbatim in the source · abstract, characters 1382–1647 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

sorghum seedling vigor was 198.21 under 6% neem extract versus 723.1 with water.

Taxon
sorghumSorghume0
Treatment
6% neem extract treatmente0
Interactor
neemAzadirachta indicae0other
Outcome
Seedling vigore0
Direction
decreaseinferrede0
Value
198.21e0
Qualifier
723.1e0
Comparator
water controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sorghum
Sorghum · Taxon · picture of the genus (one of its species)Evelyn Simak · CC BY-SA 2.0 · Wikimedia Commons ↗
Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling vigor in the water control was recorded as 287.30, 723.1, 696.6, 348.6, and 232.16 for rice, sorghum, maize, black gram, and finger millet, respectively, while values in the 6% neem extract treatment were 161.2, 198.21, 226.4, 58.9, and 62.5, respectively.”

✓ located verbatim in the source · abstract, characters 1382–1647 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

maize seedling vigor was 226.4 under 6% neem extract versus 696.6 with water.

Taxon
maizeZea mayse0
Treatment
6% neem extract treatmente0
Interactor
neemAzadirachta indicae0other
Outcome
Seedling vigore0
Direction
decreaseinferrede0
Value
226.4e0
Qualifier
696.6e0
Comparator
water controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling vigor in the water control was recorded as 287.30, 723.1, 696.6, 348.6, and 232.16 for rice, sorghum, maize, black gram, and finger millet, respectively, while values in the 6% neem extract treatment were 161.2, 198.21, 226.4, 58.9, and 62.5, respectively.”

✓ located verbatim in the source · abstract, characters 1382–1647 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

black gram seedling vigor was 58.9 under 6% neem extract versus 348.6 with water.

Taxon
black gramVigna mungoe0
Treatment
6% neem extract treatmente0
Interactor
neemAzadirachta indicae0other
Outcome
Seedling vigore0
Direction
decreaseinferrede0
Value
58.9e0
Qualifier
348.6e0
Comparator
water controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna mungo
Vigna mungo · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗
Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling vigor in the water control was recorded as 287.30, 723.1, 696.6, 348.6, and 232.16 for rice, sorghum, maize, black gram, and finger millet, respectively, while values in the 6% neem extract treatment were 161.2, 198.21, 226.4, 58.9, and 62.5, respectively.”

✓ located verbatim in the source · abstract, characters 1382–1647 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

finger millet seedling vigor was 62.5 under 6% neem extract versus 232.16 with water.

Taxon
finger milletEleusine coracanae0
Treatment
6% neem extract treatmente0
Interactor
neemAzadirachta indicae0other
Outcome
Seedling vigore0
Direction
decreaseinferrede0
Value
62.5e0
Qualifier
232.16e0
Comparator
water controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Eleusine coracana
Eleusine coracana · TaxonAgnieszka Kwiecień, Nova · CC BY-SA 4.0 · Wikimedia Commons ↗
Azadirachta indica
Azadirachta indica · InteractorVinayaraj · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling vigor in the water control was recorded as 287.30, 723.1, 696.6, 348.6, and 232.16 for rice, sorghum, maize, black gram, and finger millet, respectively, while values in the 6% neem extract treatment were 161.2, 198.21, 226.4, 58.9, and 62.5, respectively.”

✓ located verbatim in the source · abstract, characters 1382–1647 · show in text ↑

Azadirachta indica · growth · SorghumPositive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Azadirachta indica · growth · Vigna mungo (black gram)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

3 in the opposite direction

  1. positive
  2. positive
  3. positive
See all 4 papers →

Azadirachta indica · growth · Oryza sativa (rice)Positive and negative results

10 papers: 9 positive, 1 negative or no effect

9 in the opposite direction

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

Azadirachta indica · growth · Zea mays (maize)Positive and negative results

16 papers: 13 positive, 3 negative or no effect

2 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

botanical · growth · Eleusine coracana (finger millet)Positive and negative results

9 papers: 6 positive, 3 negative or no effect

2 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

botanical · growth · Vigna mungo (black gram)Positive and negative results

38 papers: 27 positive, 11 negative or no effect

10 papers in the same direction as this paper · 27 in the opposite direction

  1. positive
  2. positive
  3. positive

34 papers more in this group

See all 38 papers →

botanical · growth · SorghumPositive and negative results

57 papers: 31 positive, 26 negative or no effect

25 papers in the same direction as this paper · 31 in the opposite direction

  1. positive
  2. positive
  3. positive

53 papers more in this group

See all 57 papers →

botanical · growth · Oryza sativa (rice)Positive and negative results

211 papers: 133 positive, 78 negative or no effect

77 papers in the same direction as this paper · 133 in the opposite direction

  1. positive
  2. positive
  3. positive

207 papers more in this group

See all 211 papers →

8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.