Abstract
Abstract BACKGROUND Biocontrol is a promising strategy in the control of rice blast disease. In the present study, we isolated and characterized a novel antagonist to the pathogen Magnaporthe oryzae from rice endophytic actinomycetes. RESULTS Out of 482 endophytic actinomycetes isolated from rice blast infected and healthy rice, Streptomyces endus OsiSh ‐2 exhibited remarkable in vitro antagonistic activity. Scanning electron microscopy observations of M. oryzae treated by OsiSh ‐2 revealed significant morphological alterations in hyphae. In 2‐year field tests, the spraying of OsiSh ‐2 spore solution (10 7 spores mL −1 ) is capable of reducing rice blast disease severity by 59.64%. In addition, a fermentation broth of OsiSh ‐2 and its cell‐free filtrates could inhibit the growth of M. oryzae , suggesting the presence of active enzymes and secondary metabolites. OsiSh ‐2 tested positive for polyketide synthase‐I and nonribosomal peptide synthetase genes and can produce cellulase, protease, gelatinase, siderophore, indole‐3‐acetic acid and 1‐amino‐cyclopropane‐1‐carboxylate deaminase. A preliminary separation indicated that the methanol extract of OsiSh ‐2 could suppress the growth of pathogens. The major active component was identified as nigericin. CONCLUSION Endophytic S. endus OsiSh ‐2 has potential as a biocontrol agent against rice blast in agriculture. © 2016 Society of Chemical Industry
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Claims 9
Claim · model-written summary
Streptomyces endus OsiSh-2 showed in vitro antagonism among rice endophytes.
- Taxon
- rice
Oryza sativae0 - Interactor
- Streptomyces endus OsiSh ‐2
Streptomyces hygroscopicuse0biocontrol_agent - Outcome
- in vitro antagonistic activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of 482 endophytic actinomycetes isolated from rice blast infected and healthy rice, Streptomyces endus OsiSh ‐2 exhibited remarkable in vitro antagonistic activity.”
Claim · model-written summary
OsiSh-2 treatment significantly altered M. oryzae hyphal morphology.
- Taxon
- riceOryza sativat0
- Treatment
- treated by OsiSh ‐2e0
- Interactor
- M. oryzae
Pyricularia oryzaee0pathogen - Second interactor
- OsiSh ‐2e0biocontrol_agent
- Outcome
- morphological alterations in hyphaee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Scanning electron microscopy observations of M. oryzae treated by OsiSh ‐2 revealed significant morphological alterations in hyphae.”
t0“rice blast disease”
Claim · model-written summary
Spraying OsiSh-2 spores reduced rice blast severity by 59.64% in field tests.
- Taxon
- riceOryza sativae0
- Treatment
- spraying of OsiSh ‐2 spore solution (10 7 spores mL −1 )e0
- Interactor
- OsiSh ‐2e0biocontrol_agent
- Outcome
- rice blast disease severitye0
- Direction
- reducinge0
- Value
- 59.64%e0
- Setting
- field testse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In 2‐year field tests, the spraying of OsiSh ‐2 spore solution (10 7 spores mL −1 ) is capable of reducing rice blast disease severity by 59.64%.”
Claim · model-written summary
OsiSh-2 fermentation broth inhibited M. oryzae growth.
- Taxon
- riceOryza sativat0
- Treatment
- fermentation broth of OsiSh ‐2e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Second interactor
- OsiSh ‐2e0biocontrol_agent
- Outcome
- growth of M. oryzaee0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a fermentation broth of OsiSh ‐2 and its cell‐free filtrates could inhibit the growth of M. oryzae”
t0“rice blast disease”
Claim · model-written summary
OsiSh-2 cell-free filtrates inhibited M. oryzae growth.
- Taxon
- riceOryza sativat0
- Treatment
- cell‐free filtratese0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Second interactor
- OsiSh ‐2e0biocontrol_agent
- Outcome
- growth of M. oryzaee0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a fermentation broth of OsiSh ‐2 and its cell‐free filtrates could inhibit the growth of M. oryzae”
t0“rice blast disease”
Claim · model-written summary
OsiSh-2 tested positive for polyketide synthase-I and nonribosomal peptide synthetase genes.
- Taxon
- riceOryza sativat0
- Interactor
- OsiSh ‐2e0biocontrol_agent
- Gene
- polyketide synthase‐I and nonribosomal peptide synthetase genese0
- Outcome
- tested positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsiSh ‐2 tested positive for polyketide synthase‐I and nonribosomal peptide synthetase genes”
t0“rice blast disease”
Claim · model-written summary
OsiSh-2 produced cellulase, protease, gelatinase, siderophore, indole-3-acetic acid and ACC deaminase.
- Taxon
- riceOryza sativat0
- Outcome
- cellulase, protease, gelatinase, siderophore, indole‐3‐acetic acid and 1‐amino‐cyclopropane‐1‐carboxylate deaminasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can produce cellulase, protease, gelatinase, siderophore, indole‐3‐acetic acid and 1‐amino‐cyclopropane‐1‐carboxylate deaminase.”
t0“rice blast disease”
Claim · model-written summary
Methanol extract of OsiSh-2 suppressed pathogen growth.
- Taxon
- riceOryza sativat0
- Treatment
- methanol extract of OsiSh ‐2e0
- Interactor
- OsiSh ‐2e0biocontrol_agent
- Outcome
- growth of pathogense0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the methanol extract of OsiSh ‐2 could suppress the growth of pathogens”
t0“rice blast disease”
Claim · model-written summary
Nigericin was identified as the major active component.
- Taxon
- riceOryza sativat0
- Outcome
- major active component was identified as nigericine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major active component was identified as nigericin.”
t0“rice blast disease”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.