Abstract
CRISPR-Cas9 genome editing systems have revolutionized plant gene functional studies by enabling the targeted introduction of insertion-deletions (INDELs) via the nonhomologous end-joining (NHEJ) pathway. Frameshift-inducing INDELs can introduce a premature termination codon and, in other instances, can lead to the appearance of new proteins. Here, we found that mutations in the rice jasmonate (JA) signaling gene OsJAZ10 by CRISPR-Cas9-based genome editing did not affect canonical JA signaling. However, a type of mutant with an INDEL that yielded a novel frameshift protein named FJ10 ( F rameshift mutation of J AZ 10 ), exhibited enhanced rice growth and increased resistance to brown planthopper attacks. Overexpression of FJ10 in wild-type plants phenocopies OsJAZ10 frameshift mutants. Further characterization revealed that FJ10 interacts with Slender Rice 1 (OsSLR1) and F-box/Kelch 16 (OsFBK16). These interactions disrupt the function of OsSLR1 in suppressing gibberellin-mediated growth and the function of OsFBK16 in repressing lignin-mediated defense responses, respectively. Field experiments with FJ10 -expressing plants demonstrate that this protein uncouples the growth–defense tradeoff, opening broad avenues to obtain cultivars with enhanced yield without compromised defenses.
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Claims 9
Claim · model-written summary
OsJAZ10 editing did not affect canonical JA signaling.
- Taxon
- rice
Oryza sativae0 - Treatment
- mutations in the rice jasmonate (JA) signaling gene OsJAZ10e0
- Gene
- OsJAZ10e0
- Outcome
- canonical JA signalinge0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in the rice jasmonate (JA) signaling gene OsJAZ10 by CRISPR-Cas9-based genome editing did not affect canonical JA signaling”
Claim · model-written summary
The FJ10 frameshift mutant showed enhanced rice growth.
- Taxon
- riceOryza sativae0
- Material
- a type of mutant with an INDELe0
- Gene
- FJ10e0
- Outcome
- rice growthe0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a type of mutant with an INDEL that yielded a novel frameshift protein named FJ10 ( F rameshift mutation of J AZ 10 ), exhibited enhanced rice growth”
Claim · model-written summary
The FJ10 frameshift mutant had increased resistance to brown planthopper attacks.
- Taxon
- riceOryza sativat0
- Interactor
- brown planthopper
Nilaparvata lugense0pest - Gene
- FJ10e0
- Outcome
- resistance to brown planthopper attackse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a type of mutant with an INDEL that yielded a novel frameshift protein named FJ10 ( F rameshift mutation of J AZ 10 ), exhibited enhanced rice growth and increased resistance to brown planthopper attacks”
t0“rice”
Claim · model-written summary
FJ10 overexpression in wild-type plants reproduced the OsJAZ10 frameshift mutant phenotype.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of FJ10e0
- Gene
- FJ10e0
- Outcome
- phenocopies OsJAZ10 frameshift mutantse0
- Comparator
- OsJAZ10 frameshift mutantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FJ10 in wild-type plants phenocopies OsJAZ10 frameshift mutants”
t0“rice”
Claim · model-written summary
FJ10 interacts with OsSLR1.
- Taxon
- riceOryza sativat0
- Gene
- FJ10e0
- Outcome
- interacts with Slender Rice 1 (OsSLR1)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FJ10 interacts with Slender Rice 1 (OsSLR1) and F-box/Kelch 16 (OsFBK16)”
t0“rice”
Claim · model-written summary
FJ10 interacts with OsFBK16.
- Taxon
- riceOryza sativat0
- Gene
- FJ10e0
- Outcome
- F-box/Kelch 16 (OsFBK16)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FJ10 interacts with Slender Rice 1 (OsSLR1) and F-box/Kelch 16 (OsFBK16)”
t0“rice”
Claim · model-written summary
FJ10 interaction disrupts OsSLR1 suppression of gibberellin-mediated growth.
- Taxon
- riceOryza sativat0
- Gene
- OsSLR1e0
- Outcome
- gibberellin-mediated growthe0
- Qualifier
- disrupt the function of OsSLR1 in suppressing gibberellin-mediated growthe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These interactions disrupt the function of OsSLR1 in suppressing gibberellin-mediated growth”
t0“rice”
Claim · model-written summary
FJ10 interaction disrupts OsFBK16 repression of lignin-mediated defense responses.
- Taxon
- riceOryza sativat0
- Gene
- OsFBK16e0
- Outcome
- lignin-mediated defense responsese0
- Qualifier
- repressing lignin-mediated defense responsese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the function of OsFBK16 in repressing lignin-mediated defense responses”
t0“rice”
Claim · model-written summary
Field experiments indicated FJ10 uncouples the growth-defense tradeoff.
- Taxon
- riceOryza sativat0
- Gene
- FJ10e0
- Outcome
- uncouples the growth–defense tradeoffe0
- Setting
- Field experimentse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Field experiments with FJ10 -expressing plants demonstrate that this protein uncouples the growth–defense tradeoff”
t0“rice”
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See all 71 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.