Abstract
Grapevine Pinot gris virus (GPGV) is an emerging grapevine virus associated with grapevine leaf mottling and deformation (GLMD) disease. Being a recently identified virus, the molecular biology, pathological properties, and etiological complexity of GPGV remain poorly studied. Previous research revealed that GPGV comprises genetically different variants, some encoding a larger movement protein (MP) and others a shorter MP due to a C/T polymorphic site in ORF2 encoding MP. Variants that encode the shorter MP are associated with severe disease, whereas variants encoding the longer MP are associated with mild or no symptoms. However, this has yet to be demonstrated experimentally. Here, we report the construction of a wildtype cDNA clone, pGPGV-SY, based on ON93-12, a local isolate from Syrah closely related to the variants encoding the larger MP. Surprisingly, our clone exhibited significantly faster replication and caused more severe disease symptoms than pRI::GPGV-lat, an Italian GPGV clone, with a longer MP and demonstrated similar efficacies with that of pRI::GPGV-vir, another Italian clone with a shorter MP. A single C to T mutation at the polymorphic site of pGPGV-SY resulted in a two-fold higher RNA accumulation in the grapevine. Findings from this work constitute a leap toward the long-standing and complex question pertaining to the relationship between GPGV variant groups and GLMD. Integrating findings from this work and those by others, we propose an updated model to explain the complex relationship between GPGV variants and GLMD. • GPGV isolates of Ontario, Canada encodes a longer movement protein. • The pGPGV-SY showed higher virus RNA accumulation than pRI::GPGV-lat although both encode longer form of movement protein. • A C to T mutation which modifies movement protein size also contributes in increased virus replication and pathogenicity. • The infection of GPGV impacts on virus RNA level, symptom development and recovery, symptom severity, budbursts and yield.
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Claims 11
Claim · model-written summary
A wildtype GPGV cDNA clone pGPGV-SY was constructed from the local Syrah isolate ON93-12.
- Taxon
- grapevine Pinot gris virusGrapevine Pinot gris virust0
- Material
- pGPGV-SYe0
- Outcome
- construction of a wildtype cDNA clonee0
- Qualifier
- based on ON93-12e0
Not reportedComparator
Verbatim
e0“Here, we report the construction of a wildtype cDNA clone, pGPGV-SY, based on ON93-12, a local isolate from Syrah closely related to the variants encoding the larger MP.”
t0“grapevine Pinot gris virus”
Claim · model-written summary
pGPGV-SY replicated significantly faster than pRI::GPGV-lat.
Verbatim
e0“Surprisingly, our clone exhibited significantly faster replication and caused more severe disease symptoms than pRI::GPGV-lat, an Italian GPGV clone, with a longer MP and demonstrated similar efficacies with that of pRI::GPGV-vir, another Italian clone with a shorter MP.”
e1“a wildtype cDNA clone, pGPGV-SY”
t0“grapevine Pinot gris virus”
Claim · model-written summary
pGPGV-SY caused more severe disease symptoms than pRI::GPGV-lat.
- Treatment
- pGPGV-SYe1
- Interactor
- GPGVe0pathogen
- Outcome
- disease symptomse0
- Direction
- more severee0
- Comparator
- pRI::GPGV-late0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Surprisingly, our clone exhibited significantly faster replication and caused more severe disease symptoms than pRI::GPGV-lat, an Italian GPGV clone, with a longer MP and demonstrated similar efficacies with that of pRI::GPGV-vir, another Italian clone with a shorter MP.”
e1“a wildtype cDNA clone, pGPGV-SY”
e2“A single C to T mutation at the polymorphic site of pGPGV-SY resulted in a two-fold higher RNA accumulation in the grapevine.”
Claim · model-written summary
pGPGV-SY demonstrated efficacy similar to pRI::GPGV-vir.
Verbatim
e0“Surprisingly, our clone exhibited significantly faster replication and caused more severe disease symptoms than pRI::GPGV-lat, an Italian GPGV clone, with a longer MP and demonstrated similar efficacies with that of pRI::GPGV-vir, another Italian clone with a shorter MP.”
e1“a wildtype cDNA clone, pGPGV-SY”
t0“grapevine Pinot gris virus”
Claim · model-written summary
A C to T mutation in pGPGV-SY produced two-fold higher RNA accumulation in grapevine.
- Taxon
- grapevine Pinot gris virusGrapevine Pinot gris virust0
- Treatment
- A single C to T mutatione0
- Outcome
- RNA accumulatione0
- Direction
- highere0
- Value
- two-folde0
- Qualifier
- in the grapevinee0
Not reportedComparator
Verbatim
e0“A single C to T mutation at the polymorphic site of pGPGV-SY resulted in a two-fold higher RNA accumulation in the grapevine.”
t0“grapevine Pinot gris virus”
Claim · model-written summary
Ontario GPGV isolates encode a longer movement protein.
Verbatim
e0“GPGV isolates of Ontario, Canada encodes a longer movement protein.”
Claim · model-written summary
pGPGV-SY showed higher virus RNA accumulation than pRI::GPGV-lat despite both encoding longer movement proteins.
Verbatim
e0“The pGPGV-SY showed higher virus RNA accumulation than pRI::GPGV-lat although both encode longer form of movement protein.”
t0“grapevine Pinot gris virus”
Claim · model-written summary
The C to T mutation modifying movement-protein size also increased pathogenicity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A C to T mutation which modifies movement protein size also contributes in increased virus replication and pathogenicity.”
e1“A single C to T mutation at the polymorphic site of pGPGV-SY resulted in a two-fold higher RNA accumulation in the grapevine.”
e2“Grapevine Pinot gris virus (GPGV) is an emerging grapevine virus associated with grapevine leaf mottling and deformation (GLMD) disease.”
Claim · model-written summary
GPGV infection affects virus RNA level, symptom development and recovery.
- Treatment
- infection of GPGVe0
- Interactor
- GPGVe0pathogen
- Outcome
- virus RNA level, symptom development and recoverye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The infection of GPGV impacts on virus RNA level, symptom development and recovery, symptom severity, budbursts and yield.”
e1“Grapevine Pinot gris virus (GPGV) is an emerging grapevine virus associated with grapevine leaf mottling and deformation (GLMD) disease.”
Claim · model-written summary
GPGV infection affects symptom severity, budbursts and yield.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The infection of GPGV impacts on virus RNA level, symptom development and recovery, symptom severity, budbursts and yield.”
e1“Grapevine Pinot gris virus (GPGV) is an emerging grapevine virus associated with grapevine leaf mottling and deformation (GLMD) disease.”
Claim · model-written summary
The authors propose an updated model of the relationship between GPGV variants and GLMD.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Integrating findings from this work and those by others, we propose an updated model to explain the complex relationship between GPGV variants and GLMD.”
e1“Grapevine Pinot gris virus (GPGV) is an emerging grapevine virus associated with grapevine leaf mottling and deformation (GLMD) disease.”
The same questions in other papers
loss of function · disease · VitisPositive and negative results
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.