Abstract
The reduction of pesticide treatments is of paramount importance for the sustainability of viticulture, and it can be achieved through a combination of strategies, including the cultivation of vines ( Vitis vinifera ) that are resistant or tolerant to diseases such as downy mildew (DM). In many crops, the knock-out of Downy Mildew Resistant 6 ( DMR6 ) proved successful in controlling DM-resistance, but the effect of mutations in DMR6 genes is not yet known in grapevine. Today, gene editing serves crop improvement with small and specific mutations while maintaining the genetic background of commercially important clones. Moreover, recent technological advances allowed to produce non-transgenic grapevine clones by regeneration of protoplasts edited with the CRISPR/Cas9 ribonucleoprotein. This approach may revolutionize the production of new grapevine varieties and clones, but it requires knowledge about the targets and the impact of editing on plant phenotype and fitness in different cultivars. In this work we generated single and double knock-out mutants by editing DMR6 susceptibility (S) genes using CRISPR/Cas9, and showed that only the combined mutations in VviDMR6-1 and VviDMR6-2 are effective in reducing susceptibility to DM in two table-grape cultivars by increasing the levels of endogenous salicylic acid. Therefore, editing both genes may be necessary for effective DM control in real-world agricultural settings, which could potentially lead to unwanted phenotypes. Additional research, including trials conducted in experimental vineyards, is required to gain a deeper understanding of DMR6 -based resistance.
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Claims 3
Claim · model-written summary
Combined mutations in VviDMR6-1 and VviDMR6-2 reduced susceptibility to downy mildew in two table-grape cultivars.
- Material
- table-grape cultivarse0
- Treatment
- combined mutationse0
- Gene
- VviDMR6-1 and VviDMR6-2e0
- Outcome
- susceptibility to DMe0
- Direction
- reducinge0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in grapevine”
e0“showed that only the combined mutations in VviDMR6-1 and VviDMR6-2 are effective in reducing susceptibility to DM in two table-grape cultivars by increasing the levels of endogenous salicylic acid”
Claim · model-written summary
Reduced downy mildew susceptibility in edited grapevine is associated with increased endogenous salicylic acid.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in grapevine”
e0“showed that only the combined mutations in VviDMR6-1 and VviDMR6-2 are effective in reducing susceptibility to DM in two table-grape cultivars by increasing the levels of endogenous salicylic acid”
Claim · model-written summary
Editing both genes may be necessary for effective downy mildew control in grapevine under real-world agricultural conditions.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in grapevine”
e1“Therefore, editing both genes may be necessary for effective DM control in real-world agricultural settings”
The same questions in other papers
loss of function · disease · VitisPositive and negative results
1 paper in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.