Paper · 2000

A study of different culture media for pomegranate (Punica granatum L.) pollen

P. Melgarejo; José J. Martínez; Francisca Hernández

7
claims
14
verbatim quotes
6
citations
3
research areas
—
Not open access

Abstract

The authors tested different culture media and environmental conditions in an attempt to improve knowledge of the most suitable culture media for studying the germinative capacity of pomegranate pollen. Pollen belonging to the clone ME15, a Mollar type, was used because of its regular and abundant flowering and fruit production. The incorporation of nutrients in the medium increased the germination percentage. It was also seen that 1% agar gave better results than 1.5%. High percentages of saccharose (10-20%) did not improve germination, although 1-3% did produce notable differences. Similar improvement was observed in an experiment using 3-5-10% saccharose. The authors conclude that the optimum saccharose concentration is between 10 and 20%, with levels below 10% producing low levels of pollen germination. They also studied the effect of two temperatures (15 and 25 °C) and two incubation times (24 and 48 hours), and the possible influence of the sex of the flowers from which they took the pollen (hermaphrodite and male).

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nutrients in the culture medium increased pollen germination.

Taxon
pomegranatePunica granatumt0
Treatment
incorporation of nutrients in the mediume0
Outcome
germination percentagee0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“The incorporation of nutrients in the medium increased the germination percentage”

✓ located verbatim in the source · abstract, characters 331–412 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

One percent agar gave better germination results than 1.5%.

Taxon
pomegranatePunica granatumt0
Treatment
1% agare0
Outcome
better resultse0
Direction
bettere0
Comparator
1.5%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“1% agar gave better results than 1.5%”

✓ located verbatim in the source · abstract, characters 436–473 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

High saccharose percentages of 10-20% did not improve germination.

Taxon
pomegranatePunica granatumt0
Treatment
saccharose (10-20%)e0
Outcome
germinatione0
Direction
did not improvee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“High percentages of saccharose (10-20%) did not improve germination”

✓ located verbatim in the source · abstract, characters 475–542 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Saccharose at 1-3% produced notable differences in germination.

Taxon
pomegranatePunica granatumt0
Treatment
1-3%e0
Outcome
notable differencese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“although 1-3% did produce notable differences”

✓ located verbatim in the source · abstract, characters 544–589 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

An experiment using 3-5-10% saccharose showed similar improvement.

Taxon
pomegranatePunica granatumt0
Treatment
3-5-10% saccharosee0
Outcome
improvemente0
Direction
improvemente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“Similar improvement was observed in an experiment using 3-5-10% saccharose”

✓ located verbatim in the source · abstract, characters 591–665 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors concluded that optimum saccharose concentration is between 10 and 20%.

Taxon
pomegranatePunica granatumt0
Outcome
optimum saccharose concentration is between 10 and 20%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“The authors conclude that the optimum saccharose concentration is between 10 and 20%”

✓ located verbatim in the source · abstract, characters 667–751 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Below 10% saccharose, pollen germination was low.

Taxon
pomegranatePunica granatumt0
Treatment
levels below 10%e0
Outcome
low levels of pollen germinatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“pomegranate (Punica granatum L.)”

✓ located verbatim in the source · title, characters 39–71 · show in text ↑
e0

“levels below 10% producing low levels of pollen germination”

✓ located verbatim in the source · abstract, characters 758–817 · show in text ↑

sucrose · growth · Punica granatum (pomegranate)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

4 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

in vitro · growth · Punica granatum (pomegranate)Positive and negative results

25 papers: 24 positive, 1 negative or no effect

23 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.