Abstract
Citrus spp. represent an economically important fruit tree crop worldwide. However, molecular mechanisms underlying the interaction between citrus and the Colletotrichum gloeosporioides remain largely unexplored. In this study, we analyzed the physiological and transcriptomic changes in Citrus sinensis at different stages of incubation with C. gloeosporioides. The results indicated that C. gloeosporioides infection rapidly triggered necrosis in the epicarp of C. sinensis fruits, decreased the total flavonoid contents, and suppressed the activity of catalase, peroxidase, and superoxide dismutase enzymes. Upon inoculation with C. gloeosporioides, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours, while there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses. Through Weighted Gene Co-Expression Network Analysis, 11 key genes have been identified that could potentially play a role in the transcriptional regulation of this process, including the transcription factor bHLH189. Furthermore, the infection of C. gloeosporioides had a notable effect on both the flavonoid metabolism and the metabolic pathways related to reactive oxygen species. Our findings help to understand the interaction between citrus and C. gloeosporioides and unveil how new insights into how C. gloeosporioides circumvents citrus defense mechanisms.
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Claims 10
Claim · model-written summary
C. gloeosporioides infection rapidly caused necrosis in citrus fruit epicarp.
- Taxon
- C. sinensis
Citrus sinensise0 - Organ / stage
- epicarpe0
- Treatment
- C. gloeosporioides infectione0
- Interactor
- C. gloeosporioides
Colletotrichum gloeosporioidese0pathogen - Outcome
- necrosise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C. gloeosporioides infection rapidly triggered necrosis in the epicarp of C. sinensis fruits”
Claim · model-written summary
Infection decreased total flavonoid content.
- Taxon
- C. sinensisCitrus sinensise0
- Treatment
- C. gloeosporioides infectione0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Outcome
- total flavonoid contentse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C. gloeosporioides infection rapidly triggered necrosis in the epicarp of C. sinensis fruits, decreased the total flavonoid contents”
Claim · model-written summary
Infection suppressed catalase, peroxidase and superoxide dismutase activity.
- Taxon
- C. sinensisCitrus sinensise0
- Treatment
- C. gloeosporioides infectione0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Outcome
- activity of catalase, peroxidase, and superoxide dismutase enzymese0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C. gloeosporioides infection rapidly triggered necrosis in the epicarp of C. sinensis fruits, decreased the total flavonoid contents, and suppressed the activity of catalase, peroxidase, and superoxide dismutase enzymes”
Claim · model-written summary
After six hours of infection, 1754 genes were downregulated.
- Treatment
- inoculation with C. gloeosporioidese0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Outcome
- differentially expressed genese0
- Direction
- down-regulatede0
- Value
- 1754e0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon inoculation with C. gloeosporioides, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours”
Claim · model-written summary
After six hours of infection, 2846 genes were upregulated.
- Treatment
- inoculation with C. gloeosporioidese0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Outcome
- differentially expressed genese0
- Direction
- up-regulatede0
- Value
- 2846e0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon inoculation with C. gloeosporioides, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours”
Claim · model-written summary
Between six and twelve hours, 185 genes were downregulated.
- Outcome
- DEGse0
- Direction
- down-regulatede0
- Value
- 185e0
- Qualifier
- between six and twelve-hours post-inoculatione0
Not reportedTaxon
Verbatim
e0“there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation”
Claim · model-written summary
Between six and twelve hours, 395 genes were upregulated.
- Outcome
- DEGse0
- Direction
- up-regulatede0
- Value
- 395e0
- Qualifier
- between six and twelve-hours post-inoculatione0
Not reportedTaxon
Verbatim
e0“there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation”
Claim · model-written summary
Consistently upregulated genes were enriched for metabolism and stress responses.
Not reportedTaxonDirection
Verbatim
e0“the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses”
Claim · model-written summary
Network analysis identified 11 potentially regulatory genes, including bHLH189.
Not reportedTaxon
Verbatim
e0“11 key genes have been identified that could potentially play a role in the transcriptional regulation of this process, including the transcription factor bHLH189”
Claim · model-written summary
Infection notably affected flavonoid metabolism and reactive oxygen species pathways.
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Outcome
- flavonoid metabolism and the metabolic pathways related to reactive oxygen speciese0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the infection of C. gloeosporioides had a notable effect on both the flavonoid metabolism and the metabolic pathways related to reactive oxygen species”
The same questions in other papers
Colletotrichum gloeosporioides · disease · Citrus sinensis (sweet orange)Results agree
1 paper in the same direction as this paper
- negative
infection · quality: phytochemicals · Citrus sinensis (sweet orange)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
infection · disease · Citrus sinensis (sweet orange)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.