Abstract
Ascorbic acid (AsA), an essential non-enzymatic antioxidant in plants, regulates development growth and responses to abiotic and biotic stresses. However, research on AsA's role in cold tolerance remains largely unknown. Here, our study uncovered the positive role of AsA in improving cold stress tolerance in tomato seedlings. Physiological analysis showed that AsA significantly enhanced the enzyme activity of the antioxidant defense system in tomato seedling leaves and increased the contents of proline, sugar, abscisic acid (ABA), and endogenous AsA. In addition, we found that AsA is able to protect the photosynthetic system of tomato seedlings, thereby relieving the declining rate of chlorophyll fluorescence parameters. qRT-PCR analysis indicated that AsA significantly increased the expression of genes encoding antioxidant enzymes and involved in AsA synthesis, ABA biosynthesis/signal transduction, and low-temperature responses in tomato. In conclusion, the application of exogenous AsA enhances cold stress tolerance in tomato seedlings through various molecular and physiological responses. This provides a theoretical foundation for exploring the regulatory mechanisms underlying cold tolerance in tomato and offers practical guidance for enhancing cold tolerance in tomato cultivation.
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Claims 9
Claim · model-written summary
AsA significantly enhanced antioxidant defense enzyme activity in tomato seedling leaves.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- seedling leavese0
- Treatment
- AsAe0
- Outcome
- enzyme activity of the antioxidant defense systeme0
- Direction
- significantly enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AsA significantly enhanced the enzyme activity of the antioxidant defense system in tomato seedling leaves”
Claim · model-written summary
AsA increased proline, sugar and ABA contents in tomato seedlings.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- AsAe1
- Outcome
- contents of proline, sugar, abscisic acid (ABA)e1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AsA significantly enhanced the enzyme activity of the antioxidant defense system in tomato seedling leaves”
e1“increased the contents of proline, sugar, abscisic acid (ABA), and endogenous AsA”
Claim · model-written summary
AsA increased endogenous AsA content in tomato seedlings.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- AsAe1
- Outcome
- endogenous AsAe1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AsA significantly enhanced the enzyme activity of the antioxidant defense system in tomato seedling leaves”
e1“increased the contents of proline, sugar, abscisic acid (ABA), and endogenous AsA”
Claim · model-written summary
AsA relieved the decline of chlorophyll fluorescence parameters in tomato seedlings under cold.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- AsAe2
- Outcome
- chlorophyll fluorescence parameterse2
- Direction
- relievinge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“AsA is able to protect the photosynthetic system of tomato seedlings, thereby relieving the declining rate of chlorophyll fluorescence parameters”
Claim · model-written summary
AsA significantly increased expression of genes encoding antioxidant enzymes in tomato.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- AsAe3
- Outcome
- expression of genes encoding antioxidant enzymese3
- Direction
- significantly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“qRT-PCR analysis indicated that AsA significantly increased the expression of genes encoding antioxidant enzymes and involved in AsA synthesis, ABA biosynthesis/signal transduction, and low-temperature responses in tomato”
Claim · model-written summary
AsA significantly increased expression of genes involved in AsA synthesis in tomato.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- AsAe3
- Outcome
- involved in AsA synthesise3
- Direction
- significantly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“qRT-PCR analysis indicated that AsA significantly increased the expression of genes encoding antioxidant enzymes and involved in AsA synthesis, ABA biosynthesis/signal transduction, and low-temperature responses in tomato”
Claim · model-written summary
AsA significantly increased expression of genes in ABA biosynthesis and signal transduction in tomato.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- AsAe3
- Outcome
- ABA biosynthesis/signal transductione3
- Direction
- significantly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“qRT-PCR analysis indicated that AsA significantly increased the expression of genes encoding antioxidant enzymes and involved in AsA synthesis, ABA biosynthesis/signal transduction, and low-temperature responses in tomato”
Claim · model-written summary
AsA significantly increased expression of genes for low-temperature responses in tomato.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- AsAe3
- Outcome
- low-temperature responsese3
- Direction
- significantly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“qRT-PCR analysis indicated that AsA significantly increased the expression of genes encoding antioxidant enzymes and involved in AsA synthesis, ABA biosynthesis/signal transduction, and low-temperature responses in tomato”
Claim · model-written summary
Exogenous AsA enhances cold stress tolerance in tomato seedlings.
- Taxon
- tomatoSolanum lycopersicume4
- Treatment
- exogenous AsAe4
- Outcome
- cold stress tolerancee4
- Direction
- enhancese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“the application of exogenous AsA enhances cold stress tolerance in tomato seedlings”
The same questions in other papers
L-ascorbic acid · soil: biology · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
L-ascorbic acid · tolerance: abiotic · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
L-ascorbic acid · molecular: expression · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
L-ascorbic acid · molecular: metabolites · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
L-ascorbic acid · physiology: osmolytes · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
L-ascorbic acid · physiology: photosynthesis · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.