Abstract
Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. The effectors of phytopathogenic fungi play an important role during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are poorly understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by the infection of the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is the key pathogenic factor. Small RNA sequencing showed that the host-induced gene silencing (HIGS) vectors were processed into siRNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be a good candidate for HIGS, thereby boosting crop resistance against the pathogen and helping the limitation of R. solani infection.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Prediction identified 78 candidate effector molecules.
- Taxon
- Rhizoctonia solani AG1-IARhizoctonia solani AG-1 IAt0
- Outcome
- candidate effector moleculese0
- Value
- 78e0
Verbatim
e0“Through prediction, 78 candidate effector molecules were identified.”
t0“Rhizoctonia solani AG1-IA”
Claim · model-written summary
Eight effector RNAi constructs significantly reduced necrosis caused by GD-118 in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- eight of these constructse0
- Interactor
- AG1-IA strain GD-118e0pathogen
- Outcome
- necrosise0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight of these constructs resulted in a significant reduction in necrosis caused by the infection of the AG1-IA strain GD-118.”
t0“Nicotiana benthamiana leaves”
Claim · model-written summary
AGLIP1 suppression increased resistance of N. benthamiana and rice to GD-118.
- Taxon
- N. benthamiana and riceNicotiana benthamiana
Oryza sativae0 - Treatment
- suppression of the AGLIP1 genee0
- Interactor
- GD-118e0pathogen
- Gene
- AGLIP1e0
- Outcome
- resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118”
Claim · model-written summary
AGLIP1 suppression affected GD-118 growth rate.
- Taxon
- GD-118e0
- Treatment
- suppression of the AGLIP1 genee0
- Interactor
- GD-118e0pathogen
- Outcome
- growth rate of GD-118e0
Not reportedComparatorDirection
Verbatim
e0“The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118”
Claim · model-written summary
Host induced gene silencing vectors were processed into siRNAs in plants and transported into fungi, silencing target genes.
- Taxon
- plantsViridiplantaee0
- Outcome
- silencing of the target genese0
- Qualifier
- processed into siRNAs within the plants and then translocated to the fungie0
Verbatim
e0“the host-induced gene silencing (HIGS) vectors were processed into siRNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes.”
Claim · model-written summary
The authors propose AGLIP1 for HIGS to improve crop resistance.
- Taxon
- crope0
- Interactor
- R. solani
Rhizoctonia solanie0pathogen - Gene
- AGLIP1e0
- Outcome
- boosting crop resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AGLIP1 might be a good candidate for HIGS, thereby boosting crop resistance against the pathogen and helping the limitation of R. solani infection.”
The same questions in other papers
loss of function · disease · Nicotiana benthamianaPositive and negative results
49 papers in the same direction as this paper · 93 in the opposite direction
- negative
- negative
- negative
139 papers more in this group
See all 143 papers →loss of function · disease · Oryza sativa (rice)Positive and negative results
85 papers in the same direction as this paper · 68 in the opposite direction
- positive
- negative
- negative
150 papers more in this group
See all 154 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.