Abstract
Rice is one of the main food crops to guarantee the world's food security. Although rice is a semi-aquatic crop, long-term flooding stress can also inhibit growth and reduce yield and quality. Especially during the germination period, the germination rate of rice was inhibited by flooding stress, which resulted in the low emergence rate. Therefore, in this study, rice lines or cultivars with flood tolerance (C11) and flood sensitivity (KY60) were screened, and analyzed the response mechanisms during seed germination at different time points of waterlogging stress through transcriptome and metabolome sequencing. Compared with C11, waterlogging stress significantly inhibited the germination and coleoptile growth of KY60. Certain pathways, including plant hormones, energy metabolism, and carbohydrate metabolism, were identified as significantly involved in the flooding stress response in rice. The plant hormone-related DEGs (IAA, SAUR, ARF, LOX) and metabolites (IAA, JA, GA, and SA) were identified to up-regulate gene expression levels and significantly up-accumulate phytohormone content in C11 to resist waterlogging stress. However, the ABA signaling pathway-related genes (ZHD, PLY) and ABA content were decreased in C11 under submerged conditions. It is beneficial for C11 to promote seed germination and coleoptile elongation under flooding conditions. A total of 150 DEGs (AMY, PGLR) and 58 related compounds were identified as involved in the energy metabolism and carbohydrate metabolism of rice after flooding stress. Candidate key genes and metabolite compounds were identified by combined RNA-Seq and LC-MS/MS correlation network analysis. This study provided insights to explore the flood-tolerance mechanism of rice and laid the foundation for the cultivation of new varieties of flood-tolerance rice.
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Claims 9
Claim · model-written summary
Waterlogging significantly inhibited KY60 germination and coleoptile growth relative to C11.
- Taxon
- rice
Oryza sativat0 - Material
- KY60e0
- Treatment
- waterlogging stresse0
- Outcome
- germination and coleoptile growthe0
- Direction
- inhibitede0
- Comparator
- C11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with C11, waterlogging stress significantly inhibited the germination and coleoptile growth of KY60.”
t0“rice”
Claim · model-written summary
Plant hormone, energy and carbohydrate metabolism pathways were significantly involved in the flooding response.
- Taxon
- riceOryza sativae0
- Treatment
- flooding stresse0
- Outcome
- plant hormones, energy metabolism, and carbohydrate metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Certain pathways, including plant hormones, energy metabolism, and carbohydrate metabolism, were identified as significantly involved in the flooding stress response in rice.”
Claim · model-written summary
IAA, SAUR, ARF and LOX gene expression was upregulated in flood tolerant C11.
- Taxon
- riceOryza sativat0
- Material
- C11e0
- Gene
- IAA, SAUR, ARF, LOXe0
- Outcome
- gene expression levelse0
- Direction
- up-regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plant hormone-related DEGs (IAA, SAUR, ARF, LOX) and metabolites (IAA, JA, GA, and SA) were identified to up-regulate gene expression levels and significantly up-accumulate phytohormone content in C11 to resist waterlogging stress.”
t0“rice”
Claim · model-written summary
IAA, JA, GA and SA phytohormone content significantly accumulated in C11.
- Taxon
- riceOryza sativat0
- Material
- C11e0
- Outcome
- phytohormone contente0
- Direction
- up-accumulatee0
- Qualifier
- IAA, JA, GA, and SAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plant hormone-related DEGs (IAA, SAUR, ARF, LOX) and metabolites (IAA, JA, GA, and SA) were identified to up-regulate gene expression levels and significantly up-accumulate phytohormone content in C11 to resist waterlogging stress.”
t0“rice”
Claim · model-written summary
ABA signaling genes ZHD and PLY decreased in submerged C11.
- Taxon
- riceOryza sativat0
- Material
- C11e0
- Gene
- ZHD, PLYe0
- Outcome
- ABA signaling pathway-related genese0
- Direction
- decreasede0
- Qualifier
- under submerged conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, the ABA signaling pathway-related genes (ZHD, PLY) and ABA content were decreased in C11 under submerged conditions.”
t0“rice”
Claim · model-written summary
ABA content decreased in submerged C11.
- Taxon
- riceOryza sativat0
- Material
- C11e0
- Outcome
- ABA contente0
- Direction
- decreasede0
- Qualifier
- under submerged conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, the ABA signaling pathway-related genes (ZHD, PLY) and ABA content were decreased in C11 under submerged conditions.”
t0“rice”
Claim · model-written summary
A total of 150 DEGs including AMY and PGLR were identified in rice energy and carbohydrate metabolism after flooding.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 150 DEGs (AMY, PGLR) and 58 related compounds were identified as involved in the energy metabolism and carbohydrate metabolism of rice after flooding stress.”
Claim · model-written summary
Fifty-eight compounds related to rice energy and carbohydrate metabolism were identified after flooding.
- Taxon
- riceOryza sativae0
- Treatment
- flooding stresse0
- Outcome
- related compoundse0
- Value
- 58 related compoundse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 150 DEGs (AMY, PGLR) and 58 related compounds were identified as involved in the energy metabolism and carbohydrate metabolism of rice after flooding stress.”
Claim · model-written summary
Combined RNA-Seq and LC-MS/MS correlation networks identified candidate key genes and metabolites.
- Taxon
- riceOryza sativat0
- Outcome
- Candidate key genes and metabolite compoundse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Candidate key genes and metabolite compounds were identified by combined RNA-Seq and LC-MS/MS correlation network analysis.”
t0“rice”
The same questions in other papers
flooding · growth · Oryza sativa (rice)Positive and negative results
73 papers in the same direction as this paper · 64 in the opposite direction
- negative
- negative
- positive
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