Abstract
In vitro propagation of marigold has played a very important role in rapid multiplication of cultivars with desirable traits and production of healthy and disease-free plants. Shoot proliferation is a very important phase of micropropagation. This paper describes an efficient protocol for In vitro micropropagation of Doubled haploid lines of marigold (Tagetes erecta L.) using nodal explants. Murashige and Skoog medium (MS) supplemented with various concentrations and combinations of Benzylaminopurine (BAP), Naphthylacetic acid (NAA), Gibberellic acid (GA3) and Thidiazuron (TDZ) were employed for shoot regeneration in this study, maximum percent sprouting (100%) and minimum days to sprout break (1.48) were found in MS media supplemented with BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/L, while maximum number of sprouts (1.73) and maximum average shoot length (3.98cm) were observed in case of media supplementation with BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/L. However, in case of Thidiazuron (TDZ) minimum days taken for shoot proliferation (2.66) and maximum number of shoots per micro shoot (7.04) were observed in media supplemented with BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/L, while maximum length of shoots (4.2cm) was observed in control devoid of any hormones.
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Claims 7
Claim · model-written summary
BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/L gave maximum sprouting of 100%.
- Taxon
- Tagetes erecta L.
Tagetes erectat0 - Treatment
- BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/Le0
- Outcome
- percent sproutinge0
- Direction
- maximume0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum percent sprouting (100%) and minimum days to sprout break (1.48) were found in MS media supplemented with BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/L”
t0“Tagetes erecta L.”
Claim · model-written summary
BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/L gave minimum time to sprout break of 1.48 days.
- Taxon
- Tagetes erecta L.Tagetes erectat0
- Treatment
- BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/Le0
- Outcome
- days to sprout breake0
- Direction
- minimume0
- Value
- 1.48e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum percent sprouting (100%) and minimum days to sprout break (1.48) were found in MS media supplemented with BAP 2mg/L + NAA 0.05mg/L + GA3 1mg/L”
t0“Tagetes erecta L.”
Claim · model-written summary
BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/L gave maximum sprout number of 1.73.
- Taxon
- Tagetes erecta L.Tagetes erectat0
- Treatment
- BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/Le0
- Outcome
- number of sproutse0
- Direction
- maximume0
- Value
- 1.73e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum number of sprouts (1.73) and maximum average shoot length (3.98cm) were observed in case of media supplementation with BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/L.”
t0“Tagetes erecta L.”
Claim · model-written summary
BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/L gave maximum average shoot length of 3.98cm.
- Taxon
- Tagetes erecta L.Tagetes erectat0
- Treatment
- BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/Le0
- Outcome
- average shoot lengthe0
- Direction
- maximume0
- Value
- 3.98cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum number of sprouts (1.73) and maximum average shoot length (3.98cm) were observed in case of media supplementation with BAP 1mg/L + NAA 0.05mg/L + GA3 0.5mg/L.”
t0“Tagetes erecta L.”
Claim · model-written summary
BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/L gave minimum shoot proliferation time of 2.66 days.
- Taxon
- Tagetes erecta L.Tagetes erectat0
- Treatment
- BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/Le0
- Outcome
- days taken for shoot proliferatione0
- Direction
- minimume0
- Value
- 2.66e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“minimum days taken for shoot proliferation (2.66) and maximum number of shoots per micro shoot (7.04) were observed in media supplemented with BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/L”
t0“Tagetes erecta L.”
Claim · model-written summary
BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/L gave maximum shoots per micro shoot of 7.04.
- Taxon
- Tagetes erecta L.Tagetes erectat0
- Treatment
- BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/Le0
- Outcome
- number of shoots per micro shoote0
- Direction
- maximume0
- Value
- 7.04e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“minimum days taken for shoot proliferation (2.66) and maximum number of shoots per micro shoot (7.04) were observed in media supplemented with BAP 1mg/L + NAA 0.1mg/L + TDZ 0.5mg/L”
t0“Tagetes erecta L.”
Claim · model-written summary
Hormone-free control gave maximum shoot length of 4.2cm.
- Taxon
- Tagetes erecta L.Tagetes erectat0
- Treatment
- control devoid of any hormonese0
- Outcome
- length of shootse0
- Direction
- maximume0
- Value
- 4.2cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum length of shoots (4.2cm) was observed in control devoid of any hormones.”
t0“Tagetes erecta L.”
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