Paper · 2023 · Metamorfosa Journal of Biological Sciences

Relative Water Content and Peroxidase Enzyme Activity Lampung Local Rice in the Germination Phase Induced by Polyethylene Glycol 6000

Ayu Sasqia Putri; Lili Chrisnawati; Rochmah Agustrina; et al.

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citations
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Open access · cc-by

Abstract

Utilizing the potential of local rice is a promising venture in the development of superior drought -resistant rice. One of the local rice that comes from Lampung is the Lumbung Sewu Cantik variety. The purpose of this study was to evaluate the physiological resistance response of Lampung local rice shoots varieties Lumbung Sewu Cantik to the drought stress caused by PEG 6000 through relative water content and peroxidase enzyme activity. This study was conducted using factorial randomized design with 2 factors. Factor 1 is rice varieties (INPAGO 8 (K +), IR 64 (K-), and Lumbung Sewu Cantik). Factor 2 was the concentration of PEG 6000 (0% and 20%). Each treatment combination was repeated 3 times. Data were analyzed for variance and further analysis used DMRT test. The results showed that the drought stress did not have a significant effect on the relative water content of the rice shoots, but had a significant effect on the increase of peroxidase enzyme activity from the rice shoots. The results showed an increase in relative water content (2%) in rice shoots of Lumbung Sewu Cantik variety after being stressed by drought, while both controls experienced a decrease in relative water content. Shoots rice of Lumbung Sewu Cantik variety also experienced the highest increase in peroxidase enzyme activity (0.215 (U / mg)/min) when compared to the two controls. Furthermore, the results of DMRT test at 5% level showed that the activity of peroxidase enzyme activity from rice shoots of Lumbung Sewu Cantik variety was not much different from K- but significantly different from K+. Based on the test results of relative water content and peroxidase enzyme activity, it can be concluded that Lampung local rice, Lumbung Sewu Cantik variety, shows drought tolerance in germination phase and potential to develop into superior drought resistant rice varieties.

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Claims 9

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Drought stress had no significant effect on rice shoot relative water content.

Treatment
drought stresse0
Outcome
relative water content of the rice shootse0
Direction
did not have a significant effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“drought stress did not have a significant effect on the relative water content of the rice shoots”

✓ located verbatim in the source · abstract, characters 802–899 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Drought stress significantly increased rice shoot peroxidase activity.

Treatment
drought stresse0
Outcome
peroxidase enzyme activity from the rice shootse0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“drought stress did not have a significant effect on the relative water content of the rice shoots, but had a significant effect on the increase of peroxidase enzyme activity from the rice shoots”

✓ located verbatim in the source · abstract, characters 802–996 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lumbung Sewu Cantik shoot relative water content increased by 2% after drought stress.

Material
Lumbung Sewu Cantike0
Treatment
stressed by droughte0
Outcome
relative water contente0
Direction
increasee0
Value
2%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“an increase in relative water content (2%) in rice shoots of Lumbung Sewu Cantik variety after being stressed by drought”

✓ located verbatim in the source · abstract, characters 1017–1137 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Both control varieties decreased in shoot relative water content under drought stress.

Material
both controlse0
Treatment
Polyethylene Glycol 6000t0
Outcome
relative water contente0
Direction
decreasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“while both controls experienced a decrease in relative water content”

✓ located verbatim in the source · abstract, characters 1139–1207 · show in text ↑
t0

“Rice in the Germination Phase Induced by Polyethylene Glycol 6000”

✓ located verbatim in the source · title, characters 68–133 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lumbung Sewu Cantik shoots had the highest peroxidase activity increase, 0.215 (U / mg)/min, compared with controls.

Material
Lumbung Sewu Cantike0
Treatment
Polyethylene Glycol 6000t0
Outcome
peroxidase enzyme activitye0
Direction
highest increasee0
Value
0.215 (U / mg)/mine0
Comparator
the two controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Shoots rice of Lumbung Sewu Cantik variety also experienced the highest increase in peroxidase enzyme activity (0.215 (U / mg)/min) when compared to the two controls”

✓ located verbatim in the source · abstract, characters 1209–1374 · show in text ↑
t0

“Rice in the Germination Phase Induced by Polyethylene Glycol 6000”

✓ located verbatim in the source · title, characters 68–133 · show in text ↑
Observationobservedoriginal resultnot significant (as stated)measured

Claim · model-written summary

Lumbung Sewu Cantik peroxidase activity did not differ much from K-.

Material
Lumbung Sewu Cantike0
Outcome
peroxidase enzyme activitye0
Direction
not much differente0
Comparator
K-e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“peroxidase enzyme activity from rice shoots of Lumbung Sewu Cantik variety was not much different from K- but significantly different from K+”

✓ located verbatim in the source · abstract, characters 1454–1595 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Lumbung Sewu Cantik peroxidase activity differed significantly from K+.

Material
Lumbung Sewu Cantike0
Outcome
peroxidase enzyme activitye0
Direction
significantly differente0
Comparator
K+e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“peroxidase enzyme activity from rice shoots of Lumbung Sewu Cantik variety was not much different from K- but significantly different from K+”

✓ located verbatim in the source · abstract, characters 1454–1595 · show in text ↑
Observationauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The authors conclude Lumbung Sewu Cantik is drought tolerant during germination.

Material
Lumbung Sewu Cantike0
Organ / stage
germination phasee0
Outcome
drought tolerancee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Lumbung Sewu Cantik variety, shows drought tolerance in germination phase”

✓ located verbatim in the source · abstract, characters 1726–1799 · show in text ↑
t0

“Rice”

✓ located verbatim in the source · title, characters 68–72 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors suggest Lumbung Sewu Cantik has potential for superior drought resistant variety development.

Material
Lumbung Sewu Cantike0
Outcome
develop into superior drought resistant rice varietiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“Lampung local rice, Lumbung Sewu Cantik variety, shows drought tolerance in germination phase and potential to develop into superior drought resistant rice varieties”

✓ located verbatim in the source · abstract, characters 1706–1871 · show in text ↑
t0

“Rice in the Germination Phase Induced by Polyethylene Glycol 6000”

✓ located verbatim in the source · title, characters 68–133 · show in text ↑

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.