Abstract
Summary Barley yellow dwarf viruses (BYDVs) cause widespread damage to global cereal crops. Here we report a novel strategy for elevating resistance to BYDV infection. The 17K protein, a potent virulence factor conserved in BYDVs, interacted with barley IMP‐α1 and ‐α2 proteins that are nuclear transport receptors. Consistently, a nuclear localization signal was predicted in 17K, which was found essential for 17K to be transported into the nucleus and to interact with IMP‐α1 and ‐α2. Reducing HvIMP‐α1 and ‐α2 expression by gene silencing attenuated BYDV‐elicited dwarfism, accompanied by a lowered nuclear accumulation of 17K. Among the eight common wheat CRISPR mutants with two to four TaIMP‐α1 and ‐α2 genes mutated, the triple mutant α1 aaBBDD /α2 AAbbdd and the tetra‐mutant α1 aabbdd /α2 AAbbDD displayed strong BYDV resistance without negative effects on plant growth under field conditions. The BYDV resistance exhibited by α1 aaBBDD /α2 AAbbdd and α1 aabbdd /α2 AAbbDD was correlated with decreased nuclear accumulation of 17K and lowered viral proliferation in infected plants. Our work uncovers the function of host IMP‐α proteins in BYDV pathogenesis and generates the germplasm valuable for breeding BYDV‐resistant wheat. Appropriate reduction of IMP‐α gene expression may be broadly useful for enhancing antiviral resistance in agricultural crops and other economically important organisms.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
BYDV 17K protein interacted with barley IMP-alpha1 and IMP-alpha2.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Interactor
- BYDVs
Barley yellow dwarf viruse0pathogen - Gene
- barley IMP‐α1 and ‐α2 proteinse0
- Outcome
- interactede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The 17K protein, a potent virulence factor conserved in BYDVs, interacted with barley IMP‐α1 and ‐α2 proteins”
e1“barley”
Claim · model-written summary
The 17K nuclear localization signal was essential for nuclear transport.
- Taxon
- common wheat
Triticum aestivumt0 - Gene
- 17Ke0
- Outcome
- 17K to be transported into the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a nuclear localization signal was predicted in 17K, which was found essential for 17K to be transported into the nucleus and to interact with IMP‐α1 and ‐α2”
t0“common wheat”
Claim · model-written summary
The 17K nuclear localization signal was essential for interaction with IMP-alpha proteins.
- Taxon
- common wheatTriticum aestivumt0
- Gene
- IMP‐α1 and ‐α2e0
- Outcome
- interact with IMP‐α1 and ‐α2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a nuclear localization signal was predicted in 17K, which was found essential for 17K to be transported into the nucleus and to interact with IMP‐α1 and ‐α2”
t0“common wheat”
Claim · model-written summary
Silencing HvIMP-alpha1 and alpha2 attenuated BYDV dwarfism.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Reducing HvIMP‐α1 and ‐α2 expression by gene silencinge0
- Interactor
- BYDVBarley yellow dwarf viruse0pathogen
- Gene
- HvIMP‐α1 and ‐α2e0
- Outcome
- BYDV‐elicited dwarfisme0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reducing HvIMP‐α1 and ‐α2 expression by gene silencing attenuated BYDV‐elicited dwarfism, accompanied by a lowered nuclear accumulation of 17K”
e1“barley”
Claim · model-written summary
Silencing HvIMP-alpha1 and alpha2 lowered nuclear accumulation of 17K.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- Reducing HvIMP‐α1 and ‐α2 expression by gene silencinge0
- Outcome
- nuclear accumulation of 17Ke0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reducing HvIMP‐α1 and ‐α2 expression by gene silencing attenuated BYDV‐elicited dwarfism, accompanied by a lowered nuclear accumulation of 17K”
e1“barley”
Claim · model-written summary
Wheat triple mutant alpha1 aaBBDD/alpha2 AAbbdd strongly resisted BYDV without a plant growth penalty in the field.
- Taxon
- common wheatTriticum aestivumt0
- Material
- α1 aaBBDD /α2 AAbbdde0
- Interactor
- BYDVBarley yellow dwarf viruse0pathogen
- Outcome
- strong BYDV resistancee0
- Setting
- field conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the triple mutant α1 aaBBDD /α2 AAbbdd and the tetra‐mutant α1 aabbdd /α2 AAbbDD displayed strong BYDV resistance without negative effects on plant growth under field conditions”
t0“common wheat”
Claim · model-written summary
Wheat tetra-mutant alpha1 aabbdd/alpha2 AAbbDD strongly resisted BYDV without a plant growth penalty in the field.
- Taxon
- common wheatTriticum aestivumt0
- Material
- α1 aabbdd /α2 AAbbDDe0
- Interactor
- BYDVBarley yellow dwarf viruse0pathogen
- Outcome
- strong BYDV resistancee0
- Setting
- field conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the triple mutant α1 aaBBDD /α2 AAbbdd and the tetra‐mutant α1 aabbdd /α2 AAbbDD displayed strong BYDV resistance without negative effects on plant growth under field conditions”
t0“common wheat”
Claim · model-written summary
Resistance in the two wheat mutants correlated with decreased 17K nuclear accumulation.
- Taxon
- common wheatTriticum aestivumt0
- Interactor
- BYDVBarley yellow dwarf viruse0pathogen
- Gene
- 17Ke0
- Outcome
- BYDV resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The BYDV resistance exhibited by α1 aaBBDD /α2 AAbbdd and α1 aabbdd /α2 AAbbDD was correlated with decreased nuclear accumulation of 17K and lowered viral proliferation in infected plants”
t0“common wheat”
Claim · model-written summary
Resistance in the two wheat mutants correlated with lowered viral proliferation.
- Taxon
- common wheatTriticum aestivumt0
- Interactor
- BYDVBarley yellow dwarf viruse0pathogen
- Outcome
- BYDV resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The BYDV resistance exhibited by α1 aaBBDD /α2 AAbbdd and α1 aabbdd /α2 AAbbDD was correlated with decreased nuclear accumulation of 17K and lowered viral proliferation in infected plants”
t0“common wheat”
The same questions in other papers
loss of function · Barley yellow dwarf virus · Hordeum vulgare (barley)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
loss of function · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 10 in the opposite direction
- positive
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.