Abstract
ABSTRACT SRC2 is a C2 domain‐containing protein that responds to attack from the host and non‐host pathogens as well as abiotic stresses. The role of SRC2 in regulating viral infection is largely unknown. We employed the BYDV‐barley pathosystem to characterize the involvement of HvSRC2 in viral infection. Here, we report that 17K protein encoded by barley yellow dwarf virus‐GAV (BYDV‐GAV) interacted with Hordeum vulgare SRC2 (HvSRC2) and promoted its accumulation. Silencing of HvSRC2 expression blocked BYDV‐GAV accumulation. Transcriptome sequencing indicated that HvSRC2 regulated plant defence‐related pathways like plant hormone signal transduction, plant–pathogen interaction and other disease resistance pathways. Genes involved in plant immunity including HvRBOHC, HvRGA1, HvB‐2b, HvNPR2, HvBSK3, HvRPP13L3, HvMYC2 and HvMkk4 were upregulated upon HvSRC2 silencing. We further found that HvRBOHC interacted with HvSRC2. HvRBOHC silencing increased BYDV‐GAV accumulation, coinciding with upregulated expression of other HvRBOHs and elevated H 2 O 2 and O 2 − levels. Furthermore, we also found that MaYMV MP and TuMV viral genome‐linked protein (VPg), can both interact with HvSRC2, indicating the relative conservation between viral proteins and HvSRC2. Silencing of NbSRC2 inhibited the accumulation of TuMV and MaYMV. Taken together, our findings suggest that viral proteins utilize HvSRC2 to regulate plant defence pathways to aid viral infection.
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Claims 12
Claim · model-written summary
The BYDV-GAV 17K protein interacts with HvSRC2 and promotes its accumulation.
- Taxon
- Barley
.jpg?width=60)
Hordeum vulgaret0 - Interactor
- BYDV‐GAVe1pathogen
- Gene
- HvSRC2e1
- Outcome
- accumulatione1
- Direction
- promotede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e1“Here, we report that 17K protein encoded by barley yellow dwarf virus‐GAV (BYDV‐GAV) interacted with Hordeum vulgare SRC2 (HvSRC2) and promoted its accumulation”
Claim · model-written summary
Silencing of HvSRC2 blocks BYDV-GAV accumulation in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Silencing of HvSRC2 expressione2
- Interactor
- BYDV‐GAVe2pathogen
- Outcome
- accumulatione2
- Direction
- blockede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e2“Silencing of HvSRC2 expression blocked BYDV‐GAV accumulation”
Claim · model-written summary
Transcriptome sequencing indicates that HvSRC2 regulates disease resistance pathways in barley.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvSRC2e3
- Outcome
- disease resistance pathwayse3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e3“Transcriptome sequencing indicated that HvSRC2 regulated plant defence‐related pathways like plant hormone signal transduction, plant–pathogen interaction and other disease resistance pathways”
Claim · model-written summary
Silencing of HvSRC2 upregulates the immunity genes HvRBOHC, HvRGA1 and HvB-2b in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- HvSRC2 silencinge4
- Gene
- HvRBOHC, HvRGA1, HvB‐2be4
- Outcome
- Genes involved in plant immunitye4
- Direction
- upregulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e4“Genes involved in plant immunity including HvRBOHC, HvRGA1, HvB‐2b, HvNPR2, HvBSK3, HvRPP13L3, HvMYC2 and HvMkk4 were upregulated upon HvSRC2 silencing”
Claim · model-written summary
Silencing of HvSRC2 upregulates the immunity genes HvNPR2, HvBSK3 and HvRPP13L3 in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- HvSRC2 silencinge4
- Gene
- HvNPR2, HvBSK3, HvRPP13L3e4
- Outcome
- Genes involved in plant immunitye4
- Direction
- upregulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e4“Genes involved in plant immunity including HvRBOHC, HvRGA1, HvB‐2b, HvNPR2, HvBSK3, HvRPP13L3, HvMYC2 and HvMkk4 were upregulated upon HvSRC2 silencing”
Claim · model-written summary
Silencing of HvSRC2 upregulates the immunity genes HvMYC2 and HvMkk4 in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- HvSRC2 silencinge4
- Gene
- HvMYC2 and HvMkk4e4
- Outcome
- Genes involved in plant immunitye4
- Direction
- upregulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e4“Genes involved in plant immunity including HvRBOHC, HvRGA1, HvB‐2b, HvNPR2, HvBSK3, HvRPP13L3, HvMYC2 and HvMkk4 were upregulated upon HvSRC2 silencing”
Claim · model-written summary
HvRBOHC interacts with HvSRC2 in barley.
- Taxon
- BarleyHordeum vulgaret0
- Gene
- HvRBOHCe5
- Outcome
- interacted with HvSRC2e5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e5“We further found that HvRBOHC interacted with HvSRC2”
Claim · model-written summary
HvRBOHC silencing increases BYDV-GAV accumulation in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- HvRBOHC silencinge6
- Interactor
- BYDV‐GAVe6pathogen
- Outcome
- accumulatione6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e6“HvRBOHC silencing increased BYDV‐GAV accumulation, coinciding with upregulated expression of other HvRBOHs and elevated H 2 O 2 and O 2 − levels”
Claim · model-written summary
HvRBOHC silencing coincides with upregulated expression of other HvRBOHs in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- HvRBOHC silencinge6
- Gene
- other HvRBOHse6
- Outcome
- upregulated expression of other HvRBOHse6
- Direction
- upregulatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e6“HvRBOHC silencing increased BYDV‐GAV accumulation, coinciding with upregulated expression of other HvRBOHs and elevated H 2 O 2 and O 2 − levels”
Claim · model-written summary
HvRBOHC silencing coincides with elevated H2O2 and O2- levels in barley.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- HvRBOHC silencinge6
- Outcome
- H 2 O 2 and O 2 − levelse6
- Direction
- elevatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e6“HvRBOHC silencing increased BYDV‐GAV accumulation, coinciding with upregulated expression of other HvRBOHs and elevated H 2 O 2 and O 2 − levels”
Claim · model-written summary
The MaYMV MP and TuMV VPg viral proteins interact with HvSRC2.
- Taxon
- BarleyHordeum vulgaret0
- Interactor
- MaYMVe7pathogen
- Second interactor
- TuMV
Turnip mosaic viruse7pathogen - Gene
- HvSRC2e7
- Outcome
- interact with HvSRC2e7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e7“MaYMV MP and TuMV viral genome‐linked protein (VPg), can both interact with HvSRC2”
Claim · model-written summary
Silencing of NbSRC2 inhibits the accumulation of TuMV and MaYMV.
- Taxon
- BarleyHordeum vulgaret0
- Treatment
- Silencing of NbSRC2e7
- Interactor
- TuMVTurnip mosaic viruse7pathogen
- Second interactor
- MaYMVe7pathogen
- Gene
- NbSRC2e7
- Outcome
- accumulation of TuMV and MaYMVe7
- Direction
- inhibitede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Barley Yellow Dwarf Virus‐GAV”
e7“Silencing of NbSRC2 inhibited the accumulation of TuMV and MaYMV”
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.