Abstract
Aim: The study was undertaken with a view to standardize a protocol for in vitro regeneration of Banana cv. Grand naine using shoot tip of sucker as an explant Methodology: In present study, explants sterilized with different sterilizing agents such as Tween-20 (1%), Bavistin (0.5-1%), Streptomycin sulphate (250 mg/L), Ascorbic acid (150 mg/L) + Citric acid (100 mg/L), HgCl2 (0.1%) and 70% ethanol.Sterilized explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of plant growth hormones for shoot initiation BAP alone (1.5, 2.0, 4.0, 6.0 mg/L) and BAP (3.0 mg/L) in combination with IAA and IBA (2.0 mg/L), elongation BAP (3.0 mg/L) and NAA (1.0, 1.5, 2.0 mg/L) and rooting IAA (1.0, 1.5 mg/L) and IBA (1.0, 1.5 mg/L). Primary and secondaryhardening was done in potting mixture containing autoclaved black soil: vermicompost: cocopeat (1:1:1) and garden black soil, cocopeat and red soil (1:1:1) respectively. Results: In present investigation 1% Bavistin (fungicide) showed maximum respond to prevent fungal contamination. Highest shoot initiation (100%) was observed on a MS medium fortified with BAP (1.5 mg/L). Maximum shoot length (10.7 cm) was recorded on a MS medium supplemented with BAP (3.0 mg/L) + NAA (2.0 mg/L) + Activated charcoal. Maximum root initiation was observed on half strength MS medium supplemented with IAA (1.5 mg/L). In vitro regenerated plantlets hardened on the mixture of autoclaved black soil: vermicompost: cocopeat (1:1:1). After 14 weeks In vitro plantlets transferred in green house for acclimatization where, 80% survival rate was recorded. Conclusion: Regeneration protocol was successfully standardized. Therefore, itcan be used for large scale propagation of healthy, disease and virus free planting material and In vitro propagation helps to meet higher demand of healthy planting material within shorter period.
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Claims 7
Claim · model-written summary
1% Bavistin (fungicide) gave maximum respond prevent fungal contamination.
- Taxon
- Bananat0
- Treatment
- 1% Bavistin (fungicide)e0
- Outcome
- prevent fungal contaminatione0
- Direction
- maximum responde0
Not reportedComparator
Verbatim
e0“1% Bavistin (fungicide) showed maximum respond to prevent fungal contamination”
t0“Banana (Musa spp.) cv. Grand Naine”
Claim · model-written summary
BAP (1.5 mg/L) gave Highest shoot initiation.
Not reportedComparator
Verbatim
e0“Highest shoot initiation (100%) was observed on a MS medium fortified with BAP (1.5 mg/L)”
t0“Banana (Musa spp.) cv. Grand Naine”
Claim · model-written summary
BAP (3.0 mg/L) + NAA (2.0 mg/L) + Activated charcoal gave Maximum shoot length.
- Taxon
- Bananat0
- Treatment
- BAP (3.0 mg/L) + NAA (2.0 mg/L) + Activated charcoale0
- Outcome
- shoot lengthe0
- Direction
- Maximume0
- Value
- 10.7 cme0
Not reportedComparator
Verbatim
e0“Maximum shoot length (10.7 cm) was recorded on a MS medium supplemented with BAP (3.0 mg/L) + NAA (2.0 mg/L) + Activated charcoal”
t0“Banana (Musa spp.) cv. Grand Naine”
Claim · model-written summary
IAA (1.5 mg/L) gave Maximum root initiation.
Not reportedComparator
Verbatim
e0“Maximum root initiation was observed on half strength MS medium supplemented with IAA (1.5 mg/L)”
t0“Banana (Musa spp.) cv. Grand Naine”
Claim · model-written summary
Regenerated plantlets hardened on the stated potting mixture.
- Taxon
- Bananat0
- Treatment
- autoclaved black soil: vermicompost: cocopeat (1:1:1)e0
- Outcome
- plantlets hardenede0
Not reportedDirection
Verbatim
e0“In vitro regenerated plantlets hardened on the mixture of autoclaved black soil: vermicompost: cocopeat (1:1:1)”
t0“Banana (Musa spp.) cv. Grand Naine”
Claim · model-written summary
Plantlets had 80% survival after greenhouse acclimatization.
Verbatim
e0“80% survival rate was recorded”
t0“Banana (Musa spp.) cv. Grand Naine”
Claim · model-written summary
The regeneration protocol was standardized.
Not reportedComparatorValue
Verbatim
e0“Regeneration protocol was successfully standardized”
t0“Banana (Musa spp.) cv. Grand Naine”
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