Abstract
It is well established that potassium (K+) is an essential nutrient for wheat (Triticum aestivum L.) growth and development. Several microRNAs (miRNAs), including miR166, are reportedly vital roles related to plant growth and stress responses. In this study, a K+ starvation-responsive miRNA (miR166d) was identified, which showed increased expression in the roots of wheat seedlings exposed to low-K+ stress. The overexpression of miR166d considerably increased the tolerance of transgenic Arabidopsis plants to K+ deprivation treatment. Furthermore, disrupting miR166d expression via virus-induced gene silencing (VIGS) adversely affected wheat adaptation to low-K+ stress. Additionally, miR166d directly targeted the calcium-dependent protein kinase 7-D gene (TaCPK7-D) in wheat. The TaCPK7-D gene expression was decreased in wheat seedling roots following K+ starvation treatment. Silencing TaCPK7-D in wheat increased K+ uptake under K+ starvation. Moreover, we observed that the miR166d/TaCPK7-D module could affect wheat tolerance to K+ starvation stress by regulating TaAKT1 and TaHAK1 expression. Taken together, our results indicate that miR166d is vital for K+ uptake and K+ starvation tolerance of wheat via regulation of TaCPK7-D.
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Claims 7
Claim · model-written summary
Wheat root miR166d expression increased under low potassium.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- rootse0
- Treatment
- low-K+ stresse0
- Gene
- miR166de0
- Outcome
- expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR166d) was identified, which showed increased expression in the roots of wheat seedlings exposed to low-K+ stress”
Claim · model-written summary
Overexpressing miR166d increased transgenic Arabidopsis potassium-deprivation tolerance.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of miR166de0
- Gene
- miR166de0
- Outcome
- tolerancee0
- Direction
- increasede0
- Qualifier
- K+ deprivation treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of miR166d considerably increased the tolerance of transgenic Arabidopsis plants to K+ deprivation treatment”
Claim · model-written summary
Silencing miR166d adversely affected wheat low-potassium adaptation.
- Taxon
- wheatTriticum aestivume0
- Treatment
- virus-induced gene silencing (VIGS)e0
- Gene
- miR166de0
- Outcome
- wheat adaptation to low-K+ stresse0
- Direction
- adversely affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disrupting miR166d expression via virus-induced gene silencing (VIGS) adversely affected wheat adaptation to low-K+ stress”
Claim · model-written summary
miR166d directly targeted TaCPK7-D in wheat.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaCPK7-De0
- Outcome
- directly targetede0
- Qualifier
- miR166de0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR166d directly targeted the calcium-dependent protein kinase 7-D gene (TaCPK7-D) in wheat”
Claim · model-written summary
Potassium starvation decreased TaCPK7-D expression in wheat roots.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- rootse0
- Treatment
- K+ starvation treatmente0
- Gene
- TaCPK7-De0
- Outcome
- TaCPK7-D gene expressione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaCPK7-D gene expression was decreased in wheat seedling roots following K+ starvation treatment”
Claim · model-written summary
Silencing TaCPK7-D increased potassium uptake under starvation.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Silencing TaCPK7-De0
- Gene
- TaCPK7-De0
- Outcome
- K+ uptakee0
- Direction
- increasede0
- Qualifier
- K+ starvatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing TaCPK7-D in wheat increased K+ uptake under K+ starvation”
Claim · model-written summary
The miR166d/TaCPK7-D module affected wheat stress tolerance through TaAKT1 and TaHAK1 expression.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaAKT1 and TaHAK1e0
- Outcome
- wheat tolerance to K+ starvation stresse0
- Qualifier
- miR166d/TaCPK7-D modulee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR166d/TaCPK7-D module could affect wheat tolerance to K+ starvation stress by regulating TaAKT1 and TaHAK1 expression”
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.