Abstract
Fusarium graminearum is a destructive wheat pathogen. No fully resistant cultivars are available. Knowledge concerning the molecular weapons of F. graminearum to achieve infection remains limited. Here, we report that deletion of the putative secondary metabolite biosynthesis gene cluster fg3_54 compromises the pathogen's ability to infect wheat through cell-to-cell penetration. Ectopic expression of fgm4, a pathway-specific bANK-like regulatory gene, activates the transcription of the fg3_54 cluster in vitro. We identify a linear, C- terminally reduced and D-amino acid residue-rich octapeptide, fusaoctaxin A, as the product of the two nonribosomal peptide synthetases encoded by fg3_54. Chemically-synthesized fusaoctaxin A restores cell-to-cell invasiveness in fg3_54-deleted F. graminearum, and enables colonization of wheat coleoptiles by two Fusarium strains that lack the fg3_54 homolog and are nonpathogenic to wheat. In conclusion, our results identify fusaoctaxin A as a virulence factor required for cell-to-cell invasion of wheat by F. graminearum.
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Claims 5
Claim · model-written summary
Deleting fg3_54 compromised Fusarium graminearum infection of wheat through cell-to-cell penetration.
- Taxon
- wheat
Triticum aestivumt0e0 - Treatment
- deletion of the putative secondary metabolite biosynthesis gene cluster fg3_54e0
- Gene
- fg3_54e0
- Outcome
- pathogen's ability to infect wheat through cell-to-cell penetratione0
- Direction
- compromisese0
Not reportedInteractorComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“deletion of the putative secondary metabolite biosynthesis gene cluster fg3_54 compromises the pathogen's ability to infect wheat through cell-to-cell penetration”
Claim · model-written summary
Ectopic fgm4 expression activated fg3_54 transcription in vitro.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Ectopic expression of fgm4e0
- Gene
- fgm4e0
- Outcome
- transcription of the fg3_54 clustere0
- Direction
- activatese0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“Ectopic expression of fgm4, a pathway-specific bANK-like regulatory gene, activates the transcription of the fg3_54 cluster in vitro”
Claim · model-written summary
Fusaoctaxin A was identified as the product of two fg3_54 nonribosomal peptide synthetases.
- Taxon
- wheatTriticum aestivumt0
- Gene
- fg3_54e0
- Outcome
- fusaoctaxin Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“We identify a linear, C- terminally reduced and D-amino acid residue-rich octapeptide, fusaoctaxin A, as the product of the two nonribosomal peptide synthetases encoded by fg3_54”
Claim · model-written summary
Synthetic fusaoctaxin A restored cell-to-cell invasiveness in fg3_54-deleted Fusarium graminearum.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Chemically-synthesized fusaoctaxin Ae0
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Outcome
- cell-to-cell invasiveness in fg3_54-deleted F. graminearume0
- Direction
- restorese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
t0“wheat”
e0“Chemically-synthesized fusaoctaxin A restores cell-to-cell invasiveness in fg3_54-deleted F. graminearum”
Claim · model-written summary
Synthetic fusaoctaxin A enabled two otherwise nonpathogenic Fusarium strains to colonize wheat coleoptiles.
- Taxon
- wheatTriticum aestivumt0e0
- Interactor
- Fusarium strainse0pathogen
- Outcome
- colonization of wheat coleoptilese0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“enables colonization of wheat coleoptiles by two Fusarium strains that lack the fg3_54 homolog and are nonpathogenic to wheat”
The same questions in other papers
metabolite · organism · Triticum aestivum (wheat)Positive and negative results
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loss of function · organism · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.