Abstract
High concentrations of manganese (Mn) ions in the soil of facility-based cultivation significantly restrict the development of the cucumber industry. However, the genetic mechanisms governing Mn accumulation in crops are still not well comprehended. Through the comprehensive integration of molecular biology, epigenetic modification analysis combined with genetic analysis, we functionally characterized a novel regulatory module. Consisting of a long non-coding RNA (lncRNA, asCsMTP6) and its mitochondria-localized target Metal Tolerance Protein 6 (MTP6), it coordinately regulates Mn accumulation in cucumber. CRISPR-CsMTP6 or asCsMTP6-OE mimics toxicity, whereas CsMTP6-OE or asCsMTP6 knockdown enhances tolerance, confirming that asCsMTP6 negatively regulates CsMTP6 transcription. Additionally, the H3K27me3 methylation marks surrounding the CsMTP6 genome are reduced under Mn stress, and the inhibited expression of asCsMTP6 results in a lower level of H3K27me3 methylation in the CsMTP6 gene body and 3'UTR region, thereby facilitating the expression of CsMTP6 for tolerance to Mn stress. Furthermore, virus-induced silencing of histone methyltransferases SWN and CLF also reduces H3K27me3 methylation in the CsMTP6 genomic region, thus releasing the expression of CsMTP6. Taken together, this study demonstrates the epigenetic regulation of lncRNAs in response to Mn stress, providing new insights into the potential for developing cucumber varieties with improved tolerance to manganese-contaminated soils.
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Claims 11
Claim · model-written summary
The asCsMTP6 and MTP6 module regulates manganese accumulation in cucumber.
- Taxon
- cucumber
Cucumis sativuse0 - Gene
- asCsMTP6e0
- Outcome
- Mn accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Consisting of a long non-coding RNA (lncRNA, asCsMTP6) and its mitochondria-localized target Metal Tolerance Protein 6 (MTP6), it coordinately regulates Mn accumulation in cucumber”
Claim · model-written summary
CRISPR-CsMTP6 mimicked manganese toxicity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- CRISPR-CsMTP6e0
- Outcome
- toxicitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-CsMTP6 or asCsMTP6-OE mimics toxicity”
t0“cucumber”
Claim · model-written summary
asCsMTP6 overexpression mimicked manganese toxicity.
- Taxon
- cucumberCucumis sativust0
- Treatment
- asCsMTP6-OEe0
- Outcome
- toxicitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR-CsMTP6 or asCsMTP6-OE mimics toxicity”
t0“cucumber”
Claim · model-written summary
CsMTP6 overexpression enhanced manganese tolerance.
- Taxon
- cucumberCucumis sativust0
- Treatment
- CsMTP6-OEe0
- Outcome
- tolerancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsMTP6-OE or asCsMTP6 knockdown enhances tolerance”
t0“cucumber”
Claim · model-written summary
asCsMTP6 knockdown enhanced manganese tolerance.
- Taxon
- cucumberCucumis sativust0
- Treatment
- asCsMTP6 knockdowne0
- Outcome
- tolerancee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsMTP6-OE or asCsMTP6 knockdown enhances tolerance”
t0“cucumber”
Claim · model-written summary
asCsMTP6 negatively regulates CsMTP6 transcription.
- Taxon
- cucumberCucumis sativust0
- Gene
- asCsMTP6e0
- Outcome
- CsMTP6 transcriptione0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“asCsMTP6 negatively regulates CsMTP6 transcription”
t0“cucumber”
Claim · model-written summary
Manganese stress reduced H3K27me3 marks around CsMTP6.
- Taxon
- cucumberCucumis sativust0
- Treatment
- Mn stresse0
- Outcome
- H3K27me3 methylation marks surrounding the CsMTP6 genomee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the H3K27me3 methylation marks surrounding the CsMTP6 genome are reduced under Mn stress”
t0“cucumber”
Claim · model-written summary
Inhibited asCsMTP6 expression lowered H3K27me3 methylation in the CsMTP6 gene body and 3'UTR.
- Taxon
- cucumberCucumis sativust0
- Treatment
- inhibited expression of asCsMTP6e0
- Outcome
- H3K27me3 methylatione0
- Direction
- lowere0
- Qualifier
- CsMTP6 gene body and 3'UTR regione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibited expression of asCsMTP6 results in a lower level of H3K27me3 methylation in the CsMTP6 gene body and 3'UTR region”
t0“cucumber”
Claim · model-written summary
Reduced H3K27me3 methylation facilitated CsMTP6 expression for manganese tolerance.
- Taxon
- cucumberCucumis sativust0
- Gene
- CsMTP6e0
- Outcome
- expression of CsMTP6e0
- Qualifier
- tolerance to Mn stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thereby facilitating the expression of CsMTP6 for tolerance to Mn stress”
t0“cucumber”
Claim · model-written summary
Silencing SWN and CLF reduced H3K27me3 methylation near CsMTP6.
- Taxon
- cucumberCucumis sativust0
- Treatment
- virus-induced silencing of histone methyltransferases SWN and CLFe0
- Outcome
- H3K27me3 methylatione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced silencing of histone methyltransferases SWN and CLF also reduces H3K27me3 methylation in the CsMTP6 genomic region”
t0“cucumber”
Claim · model-written summary
Silencing SWN and CLF released CsMTP6 expression.
- Taxon
- cucumberCucumis sativust0
- Treatment
- virus-induced silencing of histone methyltransferases SWN and CLFe0
- Outcome
- expression of CsMTP6e0
- Direction
- releasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced silencing of histone methyltransferases SWN and CLF also reduces H3K27me3 methylation in the CsMTP6 genomic region, thus releasing the expression of CsMTP6”
t0“cucumber”
The same questions in other papers
loss of function · molecular: expression · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 10 in the opposite direction
- negative
- negative
- negative
8 papers more in this group
See all 12 papers →loss of function · pest · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.